A5007017280- Reconstructive Surgery and Microvascular Techniques
- Reconstructive Facial Surgery Techniques
- Pluripotent Stem Cells Research
- Mesenchymal stem cell research
- DNA Repair Mechanisms
- Periodontal Regeneration and Treatments
- Pancreatic function and diabetes
- Ubiquitin and proteasome pathways
- Cleft Lip and Palate Research
- Bone Tumor Diagnosis and Treatments
- Bone fractures and treatments
- Sarcoma Diagnosis and Treatment
- Facial Nerve Paralysis Treatment and Research
- Ear and Head Tumors
- Virus-based gene therapy research
- Tumors and Oncological Cases
- Nasal Surgery and Airway Studies
- Bone Tissue Engineering Materials
- Organ and Tissue Transplantation Research
- Urological Disorders and Treatments
- Oral and Maxillofacial Pathology
- Genomics and Chromatin Dynamics
- Osteoarthritis Treatment and Mechanisms
- Craniofacial Disorders and Treatments
- Organ Transplantation Techniques and Outcomes
Nanjing Medical University
2024
Ministry of Education
2024
Tsinghua University
2017-2021
China Medical University
2005-2018
National Yang Ming Chiao Tung University
2008-2018
China Medical University Hospital
2008-2017
Beijing Tsinghua Chang Gung Hospital
2017
National Taiwan University Hospital
2012-2013
China Medical University
2007-2012
Harvard University
2012
Over one million patients per year undergo some type of procedure involving cartilage reconstruction. Polymer hydrogels, such as alginate, have been shown to be effective carriers for chondrocytes in subcutaneous formation. The goal our current study was develop a method create complex structures (nose bridge, chin, etc.) with good dimensional tolerance form specific shapes. Molds facial implants were prepared using Silastic ERTV. Suspensions 2% alginate gelled by mixing CaSO4 (0.2 g/mL) and...
Countries with centralized, high-volume mammography screening programs, such as the U.K. and Sweden, emphasize high specificity (low percentage of false positives) sensitivity (high true positives). By contrast, United States does not have emphasizes sensitivity, has lower average specificity. We investigated whether can be achieved in context number mammograms read per radiologist (reader volume) drives both specificity.The U.K.'s National Health Service Breast Screening Programme uses...
From March of 1995 to November 1997, 95 free radial forearm flaps for orofacial reconstructions were performed and included in this prospective study donor site morbidity. All elevated using the suprafascial dissection technique. Donor closure was with either split-thickness skin grafts (64 cases) or full-thickness (31 cases). Among flaps, there two complete flap losses one partial loss because arterial thrombosis. One orocutaneus fistula successfully treated a pedicled pectoralis major...
Each year, more than one million patients undergo some type of procedure involving cartilage reconstruction. Polymer hydrogels such as alginate have been demonstrated to be effective carriers chondrocytes for subcutaneous formation. The goal this study was develop a simple method create complex structures with good three-dimensional tolerance in order form specific shapes an autologous animal model. Six implants that had seeded through injection molding process were implanted subcutaneously...
A technique of suprafascial flap elevation to prevent donor site problems is described, based on careful intraoperative observation the anatomical relations all involved structures in a series over 400 free forearm flaps used various reconstructive procedures.
The Fanconi anemia pathway is required for repair of DNA interstrand cross-links (ICL). pathway-deficient cells are hypersensitive to ICL-inducing drugs such as cisplatin. Conversely, hyperactivation the a mechanism that may underlie cellular resistance ICL agents. Modulating FANCD2 monoubiquitination, key step in pathway, be an effective therapeutic approach conferring sensitivity Here, we show inhibition Nedd8 conjugation system increases Mechanistically, inhibition, either by...
Abstract Despite a wide range of available sources for bone repair, significant limitations persist. To bioengineer bone, we have previously transferred adenovirus‐mediated human BMP‐2 gene into autologous marrow stromal cells (MSC). We successfully repaired large, full thickness, cranial defects using this approach. report now the effectiveness various hydrogels as scaffold type regeneration, comparing specifically alginate with Type I collagen. Cultured MSC miniature swine were infected or...
In therapeutic bone repairs, autologous grafts, conventional or vascularized allografts, and biocompatible artificial substitutes all have their shortcomings. The formed from peptides [recombinant human morphogenetic proteins (BMPs)], demineralized powder, a combination of both is small in size. Tissue engineering may be an alternative for cranial repair. this study, the authors developed animal model to test hypothesis that replication-defective, adenovirus-mediated BMP-2 gene transfer...
Sir:FigureVein graft interposition is an accepted technique for microvascular reconstruction. However, in head and neck free flap reconstruction, vein grafting has rarely been addressed the literature because of relative infrequency its use. In was reportedly to be used following situations: (1) when distance from defect recipient vessels greater than length pedicle; (2) vessel caliber mismatch existed between (3) there were threatened or prior failed flaps; (4) preirradiated beds; (5) tumor...
OBJECTIVE Bone marrow stromal cells (BMSCs) have great potential in bone repair. We developed an animal model to test the hypothesis that ex vivo gene transfer of human morphogenetic protein (BMP)-2 BMSCs via a replication-defective (E1A-deleted) adenovirus vector (AdV) with appropriate biopolymers would enhance autologous formation during repair large-scale skull defect. METHODS Eighteen miniature swine were treated AdV BMP-2–transduced biopolymer (group 1), 2), or alone 3). After 6 months,...
Hematopoietic lineage cells derived from human pluripotent stem (hPSCs) hold great promise for the treatment of hematological diseases and providing sufficient immune therapy. However, a simple, cost-effective method to generate large quantities hematopoietic stem/progenitor (HSPCs) is not yet available. We established monolayer, chemically defined culture system induce differentiation hPSCs in 8 days. found that insulin-free medium allowed leave pluripotency promptly preferably enter...
The anterolateral thigh (ALT) perforator flap is based on the septocutaneous or musculocutaneous perforators from lateral circumflex femoral vessels. Each artery should be accompanied by 2 veins. Anomalies of anatomy in subfascia and intramuscular layer are rarely reported. This study analyzed 6 anatomic variations subfascial to level out 1043 ALT flaps performed 2005 2007 China Medical University Hospital Taichung, Taiwan 2004 E-Da Kaohsiung, Taiwan. included (1) 1 4 accompanying veins, (2)...
Human recombinant bone morphogenetic protein 2 (rhBMP-2) accelerates regeneration but is associated with limited cementum and periodontal ligament regeneration, local root resorption, ankylosis. This study assessed a new approach to the of alveolar attachment apparatus using combination ex vivo autologous marrow mesenchymal stem cells (MSCs) engineered by replication defective adenovirus express BMP-2 gene pluronic F127 (PF127) in large mammalian animal model. Bilateral maxillary defects...
Generation of large quantities endothelial cells is highly desirable for vascular research, the treatment ischemia diseases, and tissue regeneration. To achieve this goal, we developed a simple, chemically defined culture system to efficiently rapidly differentiate from human pluripotent stem by going through an MESP1 mesoderm progenitor stage. Mesp1 key transcription factor that regulates development early cardiovascular tissue. Using MESP1-mTomato knock-in reporter embryonic cell line,...
Abstract We describe a case of complete guillotine‐type penile amputation at the proximal shaft. The blood flow was established 10 h after trauma. Circulation in replanted penis quite good but there progressive prepuce necrosis hematoma. Cosmetic and urinary outcome 6 weeks later. repair deep dorsal vessels helps corpus tissue healing glans circulation. supply from is sufficient for survival even when repaired were occluded. Surgical pitfalls replantation procedures complication management...
Abstract: Autologous vascularized bone grafts, allografts, and biocompatible artificial substitutes each have their shortcomings. Bones regenerated using recombinant human morphogenetic proteins, demineralized powder, or combinations of these are generally small do not meet the need. The current trend is to use tissue engineering approaches with marrow mesenchymal stem cells (MSCs) generate bones a desired size shape. A suspension osteogenically induced MSCs (CD11a−, CD29+, CD44+) was added...