A5049477700- Polyamine Metabolism and Applications
- Amino Acid Enzymes and Metabolism
- RNA and protein synthesis mechanisms
- Biopolymer Synthesis and Applications
- Bacteriophages and microbial interactions
- Cannabis and Cannabinoid Research
- Bacterial Genetics and Biotechnology
- Neuroscience and Neuropharmacology Research
- Chemical Synthesis and Analysis
- DNA and Nucleic Acid Chemistry
- Ion channel regulation and function
- RNA modifications and cancer
- ATP Synthase and ATPases Research
- Cancer Research and Treatments
- Molten salt chemistry and electrochemical processes
- Enzyme Structure and Function
- Fungal and yeast genetics research
- Epigenetics and DNA Methylation
- Biochemical effects in animals
- RNA Interference and Gene Delivery
- Aldose Reductase and Taurine
- Metabolism and Genetic Disorders
- Plant nutrient uptake and metabolism
- Solid-state spectroscopy and crystallography
- Peptidase Inhibition and Analysis
Chiba University
2015-2024
Amine Pharma (Japan)
2015-2024
Chiba Institute of Science
2009-2023
Kyowa-kako (Japan)
2023
Universität Hamburg
2014
Ludwig Boltzmann Institute for Lung Vascular Research
2014
Northwestern University
2014
Max Planck Institute for Heart and Lung Research
2014
Vienna General Hospital
2014
University of Giessen
2014
The site of action a Vero toxin (VT2 or Shiga‐like II) from enterohemorrhagic Escherichia coli and Shiga Shigella dysenteriae 1 on eukaryotic ribosomes was studied. Treatment with either caused the release fragment 400 nucleotides 28S ribosomal RNA when isolated treated aniline. Release this aniline treatment accompanied by inhibition protein synthesis elongation‐factor‐1‐dependent aminoacyl‐tRNA binding to ribosomes. Analysis nucleotide sequence 3′‐terminal 553 rRNA rat liver 60s subunits...
To estimate the polyamine distribution in bovine lymphocytes and rat liver, binding constants (K) for DNA, RNA, phospholipid, ATP were determined under conditions of 10 mM Tris-HCl, pH 7.5, 2 Mg2+, 150 K+. The spermine calf thymus Escherichia coli 16 S rRNA, phospholipid liver microsomes 1.15 x 10(2), 6.69 2.22 5.95 10(2) M-1, respectively. From these experimentally cellular concentrations macromolecules, ATP, polyamines, cells was estimated. In lymphocytes, mols bound to 0.79, 3.7, 0.23,...
Changes in polyamine levels during aging were measured 3-, 10- and 26-week-old female mice. The level of polyamines pancreas, brain, uterus was maintained these periods. spermidine slightly decreased intestine, significantly thymus, spleen, ovary, liver, stomach, lung, kidney, heart muscle In skin, the maximal 10-week-old mice markedly reduced results suggest that maintenance may play important roles function brain 3- to We next looked for polyamine-rich food materials as a dietary source...
The polyamine content of cells is regulated by biosynthesis, degradation and transport. In Escherichia coli, the genes for three different transport systems have been cloned characterized. Two uptake (putrescine-specific spermidine-preferential) were ABC transporters, each consisting a periplasmic substrate-binding protein, two transmembrane proteins membrane-associated ATPase. crystal structures (PotD PotF) solved. They consist domains with an alternating beta-alpha-beta topology, similar...
OBJECTIVE. The aim of our study was to evaluate the efficacy MDCT angiography in assessment lower limb peripheral arterial occlusive disease.
The amounts of normal and compensatory polyamines polyamine-requiring Escherichia coli mutants grown in the absence were determined. Although aminopropylcadaverine, a polyamine, was synthesized by MA135 (speB) DR112 (speA speB), no aminopropylcadaverine or only small EWH319 speB speC speD) MA261 (speB speC), respectively. average mass doubling times MA135, DR112, MA261, 113, 105, 260, 318 min, correlation these values with sum spermidine plus suggested that is important for cell growth...
We found previously that plasma levels of acrolein (CH2=CHCHO) and spermine oxidase (SMO) were well correlated with the degree severity chronic renal failure. The aim this study was to test whether these 2 markers acetylpolyamine (AcPAO) increased in stroke patients.The activity AcPAO SMO level protein-conjugated patients normal subjects measured by high-performance liquid chromatography ELISA, respectively. Focal infarcts estimated MRI or computed tomography (CT).The AcPAO, SMO,...
PURPOSE: To assess the ability of multi–detector row helical computed tomography (CT) to depict artery Adamkiewicz. MATERIALS AND METHODS: Seventy patients with vascular diseases underwent CT entire aorta and iliac arteries. The Adamkiewicz was examined on multiplanar curved planar reformation images cine-mode displays. visualization Adamkiewicz, as well its branching level side origin, investigated. RESULTS: In 63 (90%) 70 patients, at least a single clearly visualized from intervertebral...
Summary The functions of the putative cadaverine transport protein CadB were studied in Escherichia coli . had both uptake activity, dependent on proton motive force, and excretion acting as a cadaverine‐lysine antiporter. Km values for 20.8 303 µM respectively. Both antiporter activities functional cells. Cell growth polyamine‐requiring mutant was stimulated slightly at neutral pH by activity greatly acidic activity. At pH, operon containing cadB cadA , encoding lysine decarboxylase,...
A large number of structurally diverse compounds act as open-channel blockers NMDA receptors. They may share discrete or overlapping binding sites within the channel. In this study, effects mutations in and around membrane-spanning pore-forming regions receptor subunits were studied with three blockers, MK-801, memantine, TB-3-4, using recombinant receptors expressed in<i>Xenopus laevis</i> oocytes. Mutations at critical asparagine residues M2 loop NR1 NR2B a tryptophan residue reduced block...
We reported previously that the synthesis of specific proteins such as OppA, Cya, and RpoS (sigma(38)), which are important for cell growth viability, is stimulated by polyamines at level translation. In this study we found FecI Fis was also The enhance expression mRNAs involved in iron uptake energy metabolism rRNA some tRNAs. Shine-Dalgarno (SD) sequence their not obvious or located usual position. When SD sequences were created normal position on these mRNAs, protein no longer influenced...