A5045030990- Proteoglycans and glycosaminoglycans research
- Polyamine Metabolism and Applications
- Glycosylation and Glycoproteins Research
- Amino Acid Enzymes and Metabolism
- Polysaccharides Composition and Applications
- Bacteriophages and microbial interactions
- Carbohydrate Chemistry and Synthesis
- RNA and protein synthesis mechanisms
- Cannabis and Cannabinoid Research
- Sulfur Compounds in Biology
- Seaweed-derived Bioactive Compounds
- Fibroblast Growth Factor Research
- Epigenetics and DNA Methylation
- Connective tissue disorders research
- Marine Biology and Environmental Chemistry
- Neuroscience and Neuropharmacology Research
- Biochemical effects in animals
- Phytochemical Studies and Bioactivities
- Bacterial Genetics and Biotechnology
- RNA Interference and Gene Delivery
- Cassava research and cyanide
- Aldose Reductase and Taurine
- Biopolymer Synthesis and Applications
- Genomics, phytochemicals, and oxidative stress
- Protein Hydrolysis and Bioactive Peptides
Tokyo University of Science
2018-2025
Chiba University
2011-2021
Nihon University
2011
Amine Pharma (Japan)
2008-2010
Oita University
2010
Rakuno Gakuen University
2004
ABSTRACT A spermidine excretion protein in Escherichia coli was looked for among 33 putative drug exporters thus far identified. Cell toxicity and inhibition of growth due to overaccumulation were examined an E. strain deficient acetyltransferase, enzyme that metabolizes spermidine. Toxicity cell by recovered cells transformed with pUC mdtJI or pMW , encoding MdtJ MdtI, which belong the small multidrug resistance family exporters. Both mdtJ mdtI are necessary recovery from overaccumulated It...
The tonoplast K+ membrane transport system plays a crucial role in maintaining homeostasis plant cells. Here, we isolated cDNAs encoding two-pore channel (NtTPK1) from Nicotiana tabacum cv. SR1 and cultured BY-2 tobacco Two of the four variants NtTPK1 contained VHG GHG instead GYG signature sequence second pore region. All products were functional when expressed Escherichia coli cell membrane, was targeted to three promoter sequences N. active, expression these increased ∼2-fold by salt...
Shark fin, used as a food, is rich source of glycosaminoglyans (GAGs), acidic polysaccharides having important biological activities, suggesting their nutraceutical and pharmaceutical application. A comprehensive survey GAGs derived from the fin was performed on 11 elasmobranchs, including several deep sea sharks. Chondroitin sulfate (CS) hyaluronic acid (HA) were found in Isurus oxyrinchus, Prionace glauca, Scyliorhinus torazame, Deania calcea, Chlamydoselachus anguineus, Mitsukurina...
Abstract Serotonin (5HT) plays major roles in the physiological regulation of many behavioral processes, including sleep, feeding, and mood, but genetic mechanisms by which serotonergic neurons arise during development are poorly understood. In present study, we have investigated zebrafish. Neurons exhibiting 5HT‐immunoreactivity (5HT‐IR) detected from 45 h postfertilization (hpf) ventral hindbrain raphe, hypothalamus, pineal organ, pretectal area. Tryptophan hydroxylases encode...
The basidiomycetous mushroom Phellinus igniarius (L.) Quel. has been used as traditional medicine in various Asian countries for many years. Although reports exist on its anti-oxidative and anti-inflammatory activities therapeutic effects against diseases, our current knowledge of effect stroke is very limited. Stroke a neurodegenerative disorder which oxidative stress key hallmark. Following the 2005 discovery by Igarashi's group that acrolein produced from polyamines vivo major cause cell...
Glycomics represents one of the last frontiers and most challenging in omic analysis. Glycosylation occurs endoplasmic reticulum Golgi organelle its control is neither well-understood nor predictable based on proteomic or genomic One structurally complex classes glycoconjugates proteoglycans (PGs) their glycosaminoglycan (GAG) side chains. Previously, our laboratory solved structure chondroitin sulfate chain bikunin PG. The current study examines much more dermatan GAG decorin By utilizing...
Polypeptide release factor 2 (RF2) in Escherichia coli is known to be synthesized by a +1 frameshift at the 26th UGA codon of RF2 mRNA. Polyamines were found stimulate synthesis, an effect that was reduced excess RF2. Polyamine stimulation synthesis observed early logarithmic phase, which important phase determination overall rate cell growth. A Shine-Dalgarno-like sequence necessary for efficient but not polyamine stimulation. Spectinomycin, tetracycline, streptomycin, and neomycin...
The binding of spermine and ifenprodil to the amino terminal regulatory (R) domain N-methyl-D-aspartate receptor was studied using purified domains NR1, NR2A NR2B subunits, termed NR1-R, NR2A-R NR2B-R. R were over-expressed in Escherichia coli near homogeneity. K(d) values for [(14)C]spermine NR2B-R 19, 140, 33 microM, respectively. [(3)H]Ifenprodil bound NR1-R (K(d), 0.18 microM) 0.21 microM), but not at concentrations tested (0.1-0.8 microM). These confirmed by circular dichroism...
Abstract Adult neurogenesis in the subventricular zone of lateral ventricle decreases with age. In zone, specialized extracellular matrix structures, known as fractones, contact neural stem cells and regulate neurogenesis. Fractones are composed components, such heparan sulfate proteoglycans. We previously found that fractones capture store fibroblast growth factor 2 ( FGF ‐2) via binding, may deliver ‐2 to a timely manner. The HS ) chains aged modified based on immunohistochemistry....
Amyloid fibril formation occurs in restricted environment, such as the interface between intercellular fluids and bio-membranes. Conformational interconversion from α-helix to β-structure does not progress fluids; however, it can occur after sedimentary aggregation during amyloid induced by heat treatment of hen egg white lysozyme (HEWL). Secondary structures various proteins denatured titrated with 2,2,2-trifluoroethanol (TFE) were examined using their CD spectra. Gaussian peak/trough...
Proteins whose synthesis is enhanced by polyamines at the level of translation were identified in a polyamine-requiring mutant cultured presence 0.1% glucose and 0.02% glutamate instead 0.4% as an energy source. Under these conditions, enhancement cell growth was almost same that glucose. It found RpoN, Cra, H-NS early logarithmic phase (A(540) 0.15). The effects on H-NS, Cra due to existence unusual Shine-Dalgarno sequences (RpoN H-NS) inefficient GUG initiation codon (Cra) their mRNAs....