A5051870113- Ion Transport and Channel Regulation
- Ion channel regulation and function
- Autophagy in Disease and Therapy
- Pancreatic function and diabetes
- Cassava research and cyanide
- Trace Elements in Health
- Advanced biosensing and bioanalysis techniques
- Iron Metabolism and Disorders
- Heterotopic Ossification and Related Conditions
- Quantum-Dot Cellular Automata
- Semiconductor materials and devices
- Selenium in Biological Systems
- ATP Synthase and ATPases Research
- Hemoglobinopathies and Related Disorders
- Alkaline Phosphatase Research Studies
- Cardiac electrophysiology and arrhythmias
Shanghai Jiao Tong University
2023-2025
Shanghai Ninth People's Hospital
2023-2024
Precise regulation of intracellular phosphate (Pi) is critical for cellular function, with xenotropic and polytropic retrovirus receptor 1 (XPR1) serving as the sole Pi exporter in humans. The mechanism efflux, activated by inositol pyrophosphates (PP-IPs), has remained unclear. This study presents cryo-electron microscopy structures XPR1 multiple conformations, revealing a transmembrane pathway export dual-binding activation pattern PP-IPs. A canonical binding site located at dimeric...
Hypophosphatasia (HPP) is a metabolic bone disease that manifests as developmental abnormalities in and dental tissues. HPP patients exhibit hypo-mineralization osteopenia due to the deficiency or malfunction of tissue non-specific alkaline phosphatase (TNAP), which catalyzes hydrolysis phosphate-containing molecules outside cells, promoting deposition hydroxyapatite extracellular matrix. Despite identification hundreds pathogenic TNAP mutations, detailed molecular pathology remains unclear....
Abstract The cell maintains its intracellular pH in a narrow physiological range and disrupting the pH-homeostasis could cause dysfunctional metabolic states. Anion exchanger 2 (AE2) works at high cellular to catalyze exchange between HCO 3 − extracellular Cl , thereby maintaining pH-homeostasis. Here, we determine cryo-EM structures of human AE2 five major operating states one transitional hybrid state. Among those states, shows inward-facing, outward-facing, intermediate conformations, as...
Toehold-mediated strand displacement (TMSD) reaction is a widely used programming language in DNA nanotechnology, but its performance significantly limited by slow kinetics, especially under low-concentration reactants. Herein, we report on polyquaternium-2 (PQ2) as an effective and efficient accelerator of TMSD reaction. We show that PQ2 could drastically increase the constant 1-nt 105-fold. Significant acceleration reactions with sub-nanomolar input has been successfully demonstrated...
Abstract Anion exchanger 3 (AE3) is pivotal in regulating intracellular pH across excitable tissues, yet its structural intricacies and functional dynamics remain underexplored compared to other anion exchangers. This study unveils the insights into human AE3, including cryo-electron microscopy structures for AE3 transmembrane domains (TMD) a chimera combining N-terminal domain (NTD) with AE2 TMD (hAE3 NTD 2 ). Our analyzes reveal substrate binding site, an NTD-TMD interlock mechanism,...