A5088906257- Prostate Cancer Treatment and Research
- Bladder and Urothelial Cancer Treatments
- Prostate Cancer Diagnosis and Treatment
- Renal cell carcinoma treatment
- Urinary and Genital Oncology Studies
- Renal and related cancers
- Epigenetics and DNA Methylation
- Urological Disorders and Treatments
- Urologic and reproductive health conditions
- Cancer-related gene regulation
- Medical Imaging and Pathology Studies
- Cancer, Lipids, and Metabolism
- Urinary Bladder and Prostate Research
- Cancer Diagnosis and Treatment
- Multiple and Secondary Primary Cancers
- Inflammatory Biomarkers in Disease Prognosis
- Metastasis and carcinoma case studies
- Mechanisms of cancer metastasis
- Tissue Engineering and Regenerative Medicine
- Wnt/β-catenin signaling in development and cancer
- Bone health and treatments
- Cancer Immunotherapy and Biomarkers
- Tuberous Sclerosis Complex Research
- Genetic factors in colorectal cancer
- Pancreatic and Hepatic Oncology Research
Toranomon Hospital
2016-2025
Shimane University
2004-2022
Japanese Foundation For Cancer Research
2003-2020
Tokyo Medical Center
2020
Cancer Institute (WIA)
2008-2012
Tokyo Medical and Dental University
2012
The Cancer Institute Hospital
1997-2011
Hiroshima University
2009-2010
San Francisco VA Medical Center
2004-2009
University of California, San Francisco
2005-2009
Recent studies have shown that small noncoding RNAs, such as microRNAs and siRNAs, regulate gene expression at multiple levels including chromatin architecture, transcription, RNA editing, stability, translation. Each form of RNA-dependent regulation has been generally found to silence homologous sequences collectively called RNAi. To further study the regulatory role RNAs transcriptional level, we designed synthesized 21-nt dsRNAs targeting selected promoter regions human genes E-cadherin,...
The above article, published online on 22 April 2008 in Wiley Online Library ( wileyonlinelibrary.com ), has been retracted by agreement between the authors, journal Editor Chief, Prof. Peter Lichter, and John & Sons Ltd. retraction agreed due to errors identified Figs, 3, 4, 5. concerns about these figures cannot be resolved because original data can no longer retrieved. Reference Kikuno N , Shiina H Urakami S Kawamoto K Hirata Tanaka Y Majid Igawa M Dahiya R. . Int J Cancer 123 : 552 –...
Abstract Purpose: Cytochrome P450 1B1 (CYP1B1), a dioxin inducible member of the CYP supergene family, is overexpressed in various human malignancies including prostate cancer. We hypothesized that promoter/enhancer CpG methylation contributes to regulation CYP1B1 expression tissue. Experimental Design: Expression and induction gene clinical tissues cancer cell lines were investigated. The status was analyzed 175 96 benign prostatic hyperplasia samples using methylation-specific PCR (MSP)...
Aberrant activation of the Wingless-type (Wnt) pathway plays a significant role in pathogenesis several human cancers. Wnt inhibitory factor-1 (Wif-1) was identified as one secreted antagonists that can bind protein. We hypothesize Wif-1 an important bladder cancer pathogenesis.To test this hypothesis, epigenetic and genetic pathways involved gene modulation expression Wnt/beta-catenin-related genes were analyzed 4 tumor cell lines 54 matched normal mucosa.Wif-1 mRNA significantly enhanced...
Abstract Purpose: Aberrant promoter hypermethylation of Wnt-antagonist genes contributes to the pathogenesis several cancers. We hypothesized that combined methylation analysis could improve their use as a panel biomarkers for diagnosing and staging bladder Experimental Design: Samples (54 total) tumor corresponding normal mucosa were analyzed expression levels six (sFRP-1, sFRP-2, sFRP-4, sFRP-5, Wif-1, Dkk-3). To increase sensitivity/specificity detection, score (M score), new method...
Abstract Purpose: Bcl-2 inhibits apoptosis, and its overexpression is associated with hormone refractory prostate cancer (HRPC). Bak Bax are in the family counteract antiapoptotic function of Bcl-2. Taxane-induced (paclitaxel analogue docetaxel) phosphorylation abolishes potential effect We hypothesized that (a) survival benefit HRPC patients treated taxanes determined by presence protein (b) altered expression caused genetic mutation biological aggressiveness cancer. Experimental Design:...
Heparanase degrades heparan sulfate and has been implicated in tumor invasion metastasis. The transcription factor, early growth response 1 (EGR1), is associated with the inducible of heparanase gene. We hypothesize that CpG hypomethylation promoter coupled up-regulation EGR1 levels may induce expression human prostate cancer.Cultured cancer cell lines (Du145, DuPro, LNCaP, PC-3) without 5'-aza-2-deoxycytidine treatment, 177 samples, 69 benign prostatic hyperplasia (BPH) samples were used....
Abstract Purpose: We hypothesized that combined methylation analysis of Wnt antagonist genes could serve as a panel biomarkers for diagnosis, staging, and prognosis in renal cell carcinoma (RCC). Experimental Design: Samples (n = 62) RCC corresponding normal tissue (NRT) were analyzed using methylation-specific PCR six (sFRP-1, sFRP-2, sFRP-4, sFRP-5, Wif-1, Dkk-3). To increase the sensitivity/specificity detection, score (M score) multigene was developed. Receiver operator characteristic...
Abstract Purpose: Aberrant gene promoter methylation profiles have been well-studied in human prostate cancer. Therefore, we rationalize that multigene analysis could be useful as a diagnostic biomarker. We hypothesize new method of good and staging biomarker for Experimental Design: To test our hypothesis, cancer samples (170) benign prostatic hyperplasia (69) were examined by methylation-specific PCR three genes: adenomatous polyposis coli (APC), glutathione S-transferase pi (GSTP1),...
Although survivin expression has been reported in prostate cancer, the clinical significance of remains unclear. To clarify cancer we examined mRNA levels tissues, and correlated these with parameters including clinicopathological characteristics patients proliferation apoptosis within tissues.Cancer matched control tissues were obtained from prostates 150 who treated radical prostatectomy between July 1998 December 2002. Of samples, RNA could be extracted pathological confirmation 82 80...
You have accessJournal of Urology1 Apr 2008KNOCKDOWN OF ASTROCYTE ELEVATED GENE-1 INHIBITS PROSTATE CANCER PROGRESSION THROUGH UPREGULATION FOXO3a ACTIVITY Nobuyuki Kikuno, Hiroaki Shiina, Shinji Urakami, Ken Kawamoto, Hiroshi Hirata, Yuichiro Tanaka, Robert F Place, Deepa Pookot, Shahana Majid, Mikio Igawa, and Rajvir Dahiya KikunoNobuyuki Kikuno More articles by this author , ShiinaHiroaki Shiina UrakamiShinji Urakami KawamotoKen Kawamoto HirataHiroshi Hirata TanakaYuichiro Tanaka...
Abstract The incidence and mortality of prostate cancer (PC) is approximately 2‐fold higher among African‐Americans as compared to Caucasians very low in Asian. We hypothesize that inactivation GSTP1 genes through CpG methylation plays a role the pathogenesis PC, its ability serve diagnostic marker differs ethnic groups. promoter hypermethylation correlation with clinico‐pathological findings were evaluated 291 PC (Asian = 170; African‐American 44; Caucasian 77) 172 benign hypertrophy...
Abstract Multidrug resistance 1 (MDR1) gene encodes for P-glycoprotein (P-gp), a Mr 170,000 transmembrane calcium-dependent efflux pump that is inactivated in prostate cancer. We hypothesize inactivation of the MDR1 through CpG methylation contributes to pathogenesis and progression To test this hypothesis, status promoter its correlation with clinicopathological findings were evaluated 177 cancer samples 69 benign hypertrophy (BPH) samples. Cellular proliferation index apoptotic determined...
Abstract 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD; dioxin) is a toxic environmental contaminant that works through dioxin response elements (DRE) to activate gene expression. We tested the hypothesis cancer-related epigenetic changes suppress activation of cytochrome P4501A1 (CYP1A1) gene. 5-Aza-2′-deoxycytidine (5-aza-CdR), an inhibitor DNA methylation, increases TCDD-inducible CYP1A1 mRNA expression in cancerous LNCaP cells but not noncancerous PWR-1E and RWPE-1 (all human prostate cell...
To determine the combined effects of nerve growth factor (NGF) and vascular endothelial (VEGF) on regeneration bladder acellular matrix graft (BAMG) in spinal cord injury (SCI)-mediated neurogenic rats.In all, 40 female Sprague-Dawley rats were used. At 8 weeks after spinalization surgery (neurogenic bladder), they divided into five groups consisting untreated controls those whose bladders injected with either no factor, NGF (2 microg/rat), VEGF microg/rat) or both at partial BAMG...
The RAS-association domain family 1, isoform A (RASSF1A) gene is shown to be inactivated in prostate cancers. However, the molecular mechanism of silencing RASSFIA not fully understood. present study was designed investigate mechanisms inactivation RASSF1A through analysis CpG methylation and histone acetylation H3 associated with promoter region.Methylation status analyzed 131 samples cancer, 65 benign hypertrophy (BPH), human cell lines using methylation-specific PCR. Histone (acetyl-H3,...
A 16-year-old girl presented with a high fever that had persisted for more than 4 weeks. Computed tomography (CT) revealed 4-cm mass in the left adrenal gland. Clinically, there were no obvious symptoms of hormone excess; however, serum interleukin-6 (IL-6) and C-reactive protein levels significantly elevated. After laparoscopic adrenalectomy, subsided, her IL-6 level normalized. The tumor was pathologically diagnosed as adrenocortical carcinoma (ACC), Weiss score 5/9. cells immunoreactive...
Cisplatin-based multiple-drug chemotherapy is currently considered the most effective treatment for advanced and metastatic urothelial cancers. We treated 15 patients with locally cancers of upper urinary tract using cisplatin-based regimen in a neoadjuvant setting. The regimens administered were: M-VAC (methotrexate, vinblastine, doxorubicin cisplatin); MEC etoposide cisplatin), or M-VEC epirubicin cisplatin). Total nephroureterectomy was performed all response evaluated pathologically. Of...
Abstract BACKGROUND Aberrant CpG methylation profiles of gene promoters and their correlation with advanced pathologic features have been well investigated in prostate carcinoma (PC). Several case–control prospective studies revealed a positive association between current smoking PC. The authors hypothesized that influences both progression prognosis PC through hypermethylation related genes. METHODS A total 164 patients (52 current, 30 former, 82 never smokers) 69 benign prostatic...