A5007050287- Traumatic Ocular and Foreign Body Injuries
- Molecular Biology Techniques and Applications
- Restraint-Related Deaths
- Animal Virus Infections Studies
- Forensic and Genetic Research
- Virus-based gene therapy research
- Autopsy Techniques and Outcomes
- Gun Ownership and Violence Research
- Metabolomics and Mass Spectrometry Studies
- Microfluidic and Capillary Electrophoresis Applications
- Respiratory viral infections research
- Viral gastroenteritis research and epidemiology
- Mitochondrial Function and Pathology
- Advanced Chemical Sensor Technologies
- Analytical Chemistry and Sensors
- Environmental DNA in Biodiversity Studies
- Photosynthetic Processes and Mechanisms
- Cellular transport and secretion
- Virology and Viral Diseases
- Identification and Quantification in Food
- Genetic diversity and population structure
- Genetics, Aging, and Longevity in Model Organisms
- Herpesvirus Infections and Treatments
- Higher Education Learning Practices
- Genomics and Chromatin Dynamics
University of Freiburg
2006-2025
University Medical Center Freiburg
2010-2025
Research Institute of Molecular Pathology
2014-2023
Deutsche Gesellschaft für Internationale Zusammenarbeit
2022
TU Dresden
2012-2021
Otto-von-Guericke University Magdeburg
2004-2018
Institute of Solid State Physics
2017
Nanosystems Initiative Munich
2017
Bangor University
2012
University of Zurich
2006-2009
The vacuole is the main cellular storage pool, where sucrose (Suc) accumulates to high concentrations. While a limited number of vacuolar membrane proteins, such as V-type H+-ATPases and H+-pyrophosphatases, are well characterized, majority transporters still unidentified, among them transporter(s) responsible for Suc uptake release. In search novel tonoplast transporters, we used proteomic approach, analyzing fraction highly purified mesophyll vacuoles crop plant barley (Hordeum vulgare)....
The tonoplast monosaccharide transporter (TMT) family comprises three isoforms in Arabidopsis thaliana, and TMT-green fluorescent protein fusion proteins are targeted to the vacuolar membrane. TMT promoter-beta-glucuronidase plants revealed that TONOPLAST MONOSACCHARIDE TRANSPORTER1 (TMT1) TMT2 genes exhibit a tissue- cell type-specific expression pattern, whereas TMT3 is only weakly expressed. TMT1 induced by drought, salt, cold treatments sugar. During adaptation, tmt knockout lines...
Volatiles are efficient mediators of chemical communication acting universally as attractant, repellent or warning signal in all kingdoms life. Beside this broad impact volatiles have nature, scents also widely used pharmaceutical, food and cosmetic industries, so the identification new is great industrial interest. Despite importance well vast number diversity volatile compounds, there currently no comprehensive public database providing information on structure classification volatiles....
The desiccation-tolerant phenotype of angiosperm resurrection plants is thought to rely on the induction protective mechanisms that maintain cellular integrity during water loss. Two-dimensional (2D) sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis Xerophyta viscosa Baker proteome was carried out dehydration identify proteins may play a role in such mechanisms. Quantitative revealed greater number changes protein expression levels at 35% than 65% relative...
Young meristematic plant cells contain a large number of small vacuoles, while the largest part vacuome in mature is composed by central vacuole, occupying 80% to 90% cell volume. Thus far, only limited vacuolar membrane proteins have been identified and characterized. The proteomic approach powerful tool identify new proteins. To analyze vacuoles from growing tissues we isolated cauliflower (Brassica oleracea) buds, which are constituted amount but also expansion as well fully expanded...
Forensic examination of a scene where two bodies were found after fall from high-rise apartment building revealed distinct tissue imprint resembling facial contours on the asphalt between as well laceration back head and an abrasion forehead one body. Tissue imprints located away positions can indicate manipulation finding injuries in opposing body regions at least blunt traumas. Initial assessment ruled out intermediate contacts with building, pointing instead to significant horizontal...
<i>Introduction:</i> In light of the fact that, to date, no information is available about titanium relative its application in prosthetic material employed for hernial repair, aim present work was evaluate fundamental possibilities titanium-coated polypropylene meshes. <i>Materials and Methods:</i> experiments with animals, two groups, each containing 11 pigs, received either a heavyweight mesh (Atrium<sup>®</sup>) or an identical but (titanium-coated...
Bovine respiratory syncytial virus (BRSV) encodes three transmembrane envelope glycoproteins, namely the small hydrophobic (SH) protein, attachment glycoprotein (G) and fusion (F). The BRSV reverse genetics system has been used to generate viable recombinant lacking either G gene or SH both genes. deletion mutants were fully competent for multicycle growth in cell culture, proving that, of genes, genes are non-essential. Virus morphogenesis was not impaired by deletions. study role F...
Abstract Proper cholesterol transport is crucial for the functionality of cells. In C . elegans , certain derivatives called dafachronic acids (DAs) govern entry into diapause. their absence, worms form a developmentally arrested dauer larva. Thus, to appropriate places DA biosynthesis warrants reproductive growth. Recently, we discovered novel class glycosphingolipids, PEGCs, required mobilization/transport from internal storage pools. Here, identify other components involved in this...
Abstract In plants the vacuolar functions are cellular storage of soluble carbohydrates, organic acids, inorganic ions and toxic compounds. Transporters channels located in membrane, tonoplast, modulated by PTMs to facilitate optimal functioning a large number metabolic pathways. Here we present phosphoproteomic approach for identification vivo phosphorylation sites tonoplast (vacuolar membrane) proteins. Highly purified tonoplast‐enriched microsomes were isolated from photosynthetically...
The gene for proline iminopeptidase from Lactobacillus delbrueckii subsp. lactis DSM 7290 coding an enzyme that hydrolyses the synthetic substrate l-prolyl-β-naphthylamide (Pro-βNA) was cloned in Escherichia coli. An enzymic plate assay used to screen positive clones. gene, designated pepI, subcloned into vector pUC18 and sequenced. nucleotide sequence revealed 882 bp open reading frame encoding 294 amino acids, with a calculated molecular mass of 32883 Da. By cloning under control lac...
The silver(I)-promoted oxidative cyclisation of 1-propargyl-substituted tetrahydroisoquinolines affords pyrrolo[2,1-a]isoquinolines. Scope and limitations this method are described an application to the synthesis natural product (±)-crispine A is presented.
Abstract Background Various experimental techniques yield peptides that are biologically active but have unfavourable pharmacological properties. The design of structurally similar organic compounds, i.e. peptide mimetics, is a challenging field in medicinal chemistry. Results SuperMimic identifies compounds mimic parts protein, or positions proteins suitable for inserting mimetics. application provides libraries contain peptidomimetic building blocks on the one hand and protein structures...
The glucose transporter GLUT8 cycles between intracellular vesicles and the plasma membrane. Like insulin-responsive GLUT4, is primarily located in compartments under basal conditions. Whereas translocation of GLUT4 to membrane stimulated by insulin, distribution not affected insulin treatment adipose cells. However, blocking endocytosis co-expression a dominant-negative dynamin GTPase (K44A) or mutation N-terminal dileucine (LL12/13) motif leads accumulation at cell surface variety...