A5029280329- Birth, Development, and Health
- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Ovarian function and disorders
- Diet, Metabolism, and Disease
- Adipose Tissue and Metabolism
- Lipid metabolism and biosynthesis
- Aldose Reductase and Taurine
University of Antwerp
2022-2025
Diet-induced obesity can alter ovarian functions after long-term high-fat high-sugar (HFHS) diet feeding. However, the acute impact of obesogenic diets on follicular cells, and how this evolves over time when intake is continued develops, not known. Our aim was to determine onset progression changes in granulosa cell (GC) transcriptomic profile starting a HFHS-diet feeding mice. We also examined oocyte lipid droplet content (LDC) mitochondrial ultrastructural abnormalities. Swiss (outbred)...
Can diet normalization or a calorie-restricted for 2 4 weeks be used as preconception care intervention (PCCI) in Western-type diet-induced obese Swiss mice to restore metabolic health and oocyte quality?Metabolic developmental competence was already significantly improved the group after weeks, while that underwent showed quality weeks.Maternal obesity is linked with reduced quality; therefore, infertile women are advised lose weight before conception increase pregnancy chances. However,...
Metabolic disorders due to obesity and unhealthy lifestyle directly alter the oocyte's microenvironment impact oocyte quality. Oxidative stress mitochondrial dysfunction play key roles in pathogenesis. Acute effects on fully grown oocytes are evident, but early follicular stages also sensitive metabolic leading a long-term cells oocytes. Improving preconception health is therefore of capital importance research animal models has demonstrated that quality not recovered. In vitro fertilisation...
Research question How long does it take for an obesogenic (high-fat/high-sugar, HF/HS) diet to influence the oviductal microenvironment? What are affected cellular pathways and they dependent on genetic background of mouse model? Design Female Swiss (outbred) C57BL/6N (B6, inbred) mice were fed either a control (10% fat) or HF/HS (60% fat, 20% fructose) diet. Body weight was measured weekly. Mice sacrificed at 3 days (3d), 1 week (1w), 4w, 8w, 12w 16w (n = 5 per treatment time point). Total...
We investigated whether a high-fat/high-sugar (HF/HS) diet alters the lipidomic profile of oviductal epithelium (OE) and studied patterns these changes over time. Female outbred Swiss mice were fed either control (10% fat) or HF/HS (60% fat, 20% fructose) diet. Mice (n = 3 per treatment time point) sacrificed oviducts collected at days 1, 4, 8, 12 16 weeks on Lipids in OE imaged using matrix-assisted laser desorption ionisation mass spectrometry imaging. Discriminative m/z values...