A5021749170- Microbial Metabolic Engineering and Bioproduction
- Metabolomics and Mass Spectrometry Studies
- Gut microbiota and health
- Diet and metabolism studies
- Bioinformatics and Genomic Networks
- Metabolism and Genetic Disorders
- Agricultural Practices and Plant Genetics
- Agricultural pest management studies
- Biofuel production and bioconversion
- ATP Synthase and ATPases Research
- Pharmacogenetics and Drug Metabolism
- Mitochondrial Function and Pathology
- Cancer, Hypoxia, and Metabolism
- Genetics and Plant Breeding
- Hemiptera Insect Studies
- HER2/EGFR in Cancer Research
- Nematode management and characterization studies
- Salivary Gland Disorders and Functions
- Agricultural Development and Management
- Ubiquitin and proteasome pathways
- Dengue and Mosquito Control Research
- Peroxisome Proliferator-Activated Receptors
- Insect and Arachnid Ecology and Behavior
- Monoclonal and Polyclonal Antibodies Research
- Probiotics and Fermented Foods
Indian Institute of Technology Madras
2004-2023
University of Agricultural Sciences, Dharwad
2022
University of Luxembourg
2014-2020
Synthetic Biologics (United States)
2020
University of Iceland
2012-2014
Center for Systems Biology
2013
A multitude of factors contribute to complex diseases and can be measured with 'omics' methods. Databases facilitate data interpretation for underlying mechanisms. Here, we describe the Virtual Metabolic Human (VMH, www.vmh.life) database encapsulating current knowledge human metabolism within five interlinked resources 'Human metabolism', 'Gut microbiome', 'Disease', 'Nutrition', 'ReconMaps'. The VMH captures 5180 unique metabolites, 17 730 reactions, 3695 genes, 255 Mendelian diseases, 818...
The human gut microbiota consists of ten times more microorganisms than there are cells in our body, processes otherwise indigestible nutrients, and produces important energy precursors, essential amino acids, vitamins. In this study, we assembled validated a genome-scale metabolic reconstruction Bacteroides thetaiotaomicron (iAH991), prominent representative the microbiota, consisting 1488 reactions, 1152 metabolites, 991 genes. To create comprehensive model host-microbe interactions,...
Inborn errors of metabolism (IEMs) are hereditary metabolic defects, which encountered in almost all major pathways occurring man. Many IEMs screened for neonates through metabolomic analysis dried blood spot samples. To enable the mapping these data onto published human reconstruction, we added missing reactions and involved acylcarnitine (AC) fatty acid oxidation (FAO) metabolism. Using literary data, reconstructed an AC/FAO module consisting 352 139 metabolites. When this was combined...
Small intestinal epithelial cells (sIECs) have a significant share in whole body metabolism as they perform enzymatic digestion and absorption of nutrients. Furthermore, the diet plays key role number complex diseases including obesity diabetes. The impact altered genetic backgrounds on human may be studied by using computational modeling. A metabolic reconstruction sIECs was manually assembled literature. resulting sIEC model subjected to two different diets obtain condition-specific...
Objectives: Antibody-drug conjugates (ADCs) signify a major leap forward in cancer treatment, providing precise method to deliver potent cytotoxic drugs directly cells. Understanding and analyzing various aspects of ADCs are crucial for making well-informed decisions during the development these groundbreaking medications. Although generally well tolerated by patients, certain treatment-related adverse events commonly reported lead patient discontinuation. Dose-limiting toxicities (DLTs)...
Abstract The onset of colorectal cancer (CRC) is often attributed to gut bacterial dysbiosis, and thus microbiota are highly relevant in devising treatment strategies. Certain microbes, like Enterococcus spp ., exhibit remarkable anti-neoplastic probiotic properties, which can aid silver nanoparticle (AgNPs) induced reactive oxygen species (ROS)-based CRC treatment. However, the effects AgNPs on microbial metabolism have not been reported far. In this study, a detailed systems-level...
Introduction The integrity of the intestinal epithelium is crucial for human health and harmed in autism spectrum disorder (ASD). An aberrant gut microbial composition resulting gut-derived metabolic toxins was found to damage epithelium, jeopardizing tissue integrity. These further reach brain via gut-brain axis, disrupting normal function brain. A mechanistic understanding disturbances essential design effective therapeutics early intervention block disease progression. Herein, we present...
Metabolism contributes significantly to the pharmacokinetics and pharmacodynamics of a drug. In addition, diet genetics have profound effect on cellular metabolism with respect both health disease. present study, we assembled comprehensive, literature-based drug metabolic reconstruction 18 most highly prescribed groups, including statins, anti-hypertensives, immunosuppressants analgesics. This captures in detail our current understanding their absorption, intracellular distribution,...
Abstract Precision medicine is an emerging paradigm that requires realistic, mechanistic models capturing the complexity of human body. We present two comprehensive molecular to physiological-level, gender-specific whole-body metabolism (WBM) reconstructions, named Harvey, in recognition William and Harvetta. These validated, knowledge-based WBM reconstructions capture 20 organs, six sex blood cells, gastrointestinal lumen, systemic circulation, blood-brain barrier. They represent 99% body...
Abstract A multitude of factors contribute to complex diseases and can be measured with “omics” methods. Databases facilitate data interpretation for underlying mechanisms. Here, we describe the Virtual Metabolic Human (VMH, http://vmh.life) database encapsulating current knowledge human metabolism within five interlinked resources “Human metabolism”, “Gut microbiome”, “Disease”, “Nutrition”, “ReconMaps”. The VMH captures 5,180 unique metabolites, 17,730 reactions, 3,288 genes, 255 Mendelian...
Appropriate species of oleaginous bacteria, with their high growth rates and lipid accumulation capabilities, can be good contenders for industrial triacylglycerol (TAG) production, compared to microalgae. Further, oxidative stress (OS) used significantly increase TAG yields in microbes, but the mechanism is unexplored. In a first, this study explored behind OS-mediated by bacterium, Rhodococccus opacus PD630, through experimental analysis metabolic modelling. Two mechanisms that could...
Retinoblastoma (RB) is a childhood eye cancer. Currently, chemotherapy, local therapy, and enucleation are the main ways in which these tumors managed. The present work first study that uses constraint-based reconstruction analysis approaches to identify explain RB-specific survival strategies, RB tumor specific. Importantly, our model-specific secretion profile also found RB1-depleted human retinal cells vitro suggests novel biomarkers involved lipid metabolism may be important. Finally,...
High-performance torque control in switched reluctance motors (SRM) requires closed-loop with accurate and high bandwidth feedback. An analytical expression for terms of stator phase current rotor position can be used as estimator this purpose. Such an derived from SRM flux-linkage model, using the coenergy principle. We have observed that most models available literature map measured accurately. However, obtained model fail to predict In work, a physically intuitive nonlinear reported by...
Abstract Colorectal cancer (CRC) is the fourth leading cause of mortality, world-wide. Gut bacterial dysbiosis being one major causes CRC onset. microbiota produced metabolites, e.g. folate and butyrate play crucial roles in progression treatment, thus, need to be considered for effective management. A potential therapy, i.e., use silver nanoparticles (AgNPs), imparts cytotoxic effects by inducing high intracellular reactive oxygen species (ROS) levels. However, simultaneous interactions...
Pole bean (Phaseolus vulgaris L.) is an annual herb grown vertically on a support. The present study asseses the insect pests infesting this along with their natural enemies under nethouse conditions. was done at Hi-tech Horticulture Unit, UAS, Dharwad. results revealed five species of insects and one mite pole various phases crop growth. These include- serpentine leaf miner (Liriomyza trifolii), pod fly (Melanagromyza obtusa), seed (Anthomyia sp.), leafhopper (Ianagallia bifurcate), tobacco...
Abstract Autism spectrum disorder (ASD) 1 refers to the set of complex neurological disorders characterized by repetitive behaviour. The reported occurrence abnormal gut bacteria, along with prevalence gastrointestinal in ASD indicate its strong correlation microflora. Our study aims understand role diet and bacteria via an integrated constraint-based PBPK model. Genome scale models five major which were be associated ASD, human host, i.e., combined small intestinal enterocyte neuronal brain...
Abstract Reactive species (RS) are known to play significant roles in cancer development as well treating or managing cancer. On the other hand, genome scale metabolic models being used understand cell metabolism disease contexts including cancer, and also planning strategies handle diseases. Despite their crucial cancers, reactive have not been adequately modeled (GSMMs) when probing for detection of drug targets. In this work, we developed a module reactions, which is scalable - it can be...