A5003971433- Neuroendocrine Tumor Research Advances
- Pancreatic and Hepatic Oncology Research
- Ion Transport and Channel Regulation
- Lung Cancer Research Studies
- Botulinum Toxin and Related Neurological Disorders
- Neonatal Respiratory Health Research
- Renal and related cancers
- Ion channel regulation and function
- Magnesium in Health and Disease
- Peripheral Nerve Disorders
- Nitric Oxide and Endothelin Effects
- Pharmacogenetics and Drug Metabolism
- Neuroendocrine regulation and behavior
- Neurological disorders and treatments
- Eicosanoids and Hypertension Pharmacology
- Peptidase Inhibition and Analysis
- Renin-Angiotensin System Studies
- Electrolyte and hormonal disorders
- Protein Kinase Regulation and GTPase Signaling
- Drug Transport and Resistance Mechanisms
- RNA Interference and Gene Delivery
- Stroke Rehabilitation and Recovery
- Congenital Diaphragmatic Hernia Studies
- Antibiotic Resistance in Bacteria
- Cell Adhesion Molecules Research
Karolinska Institutet
1996-2021
Kista Photonics Research Center
2020-2021
Karolinska University Hospital
2021
Biosensor Applications (Sweden)
2016
Boston Children's Hospital
1996-1997
St Göran's Children's Hospital
1996
The capacity to concentrate urine develops progressively during postnatal life in most mammalian species. Here we have examined whether low expression of the arginine vasopressin (AVP)-activated water channel aquaporin-2 (AQP-2) may be a limiting factor for concentrating infant rats. Urine osmolality response 24-h dehydration increased significantly from 10 40 days age. rapid increase occurred weaning period, i.e., 15-20. A similar developmental pattern was observed AQP-2 mRNA levels renal...
At birth, rapid removal of lung liquid from potential airspaces is required to establish pulmonary gas exchange. To investigate the role for water channels, aquaporins (AQP) and ion transporters in this process, mRNA expression AQP, Na + ,K ‐ATPase amiloride‐sensitive channel (ENaC) were studied fetal postnatal rat lung. The all increased postnatally. following channels expressed lung, AQP1, 4 5. most specific perinatal induction pattern was observed AQP4. A sharp transient increase AQP4...
The enzyme Na<sup>+</sup>,K<sup>+</sup>-ATPase creates the transmembrane Na<sup>+</sup> gradient that is of vital importance for functioning all eukaryotic cells. can be phosphorylated by protein kinase A (PKA) and C (PKC), these sites phosphorylation have been identified. In present study, we examined physiological significance PKC rat Na<sup>+</sup>,K<sup>+</sup>-ATPase. COS cells transfected with wild type α1, intracellular was higher pH lower than in α1 which site, Ser-23, had mutated...
Most vital cellular functions are dependent on a fine-tuned regulation of intracellular ion homeostasis. Here we have demonstrated, using COS cells that were untransfected or transfected with wild-type rat ouabain-resistant Na + -K -ATPase, partial inhibition -ATPase has dramatic influence cell attachment to fibronectin. Ouabain dose-dependently decreased in and expressing but not ouabain-insensitive whereas by lowering extracellular K concentration all three types. Thirty percent...
Objectives The therapeutic effects of botulinum neurotoxin (BoNT) are well documented in upper limb spasticity. However, several factors may influence treatment efficacy, including targeting neuromuscular junctions (NMJs). We examined whether NMJ-targeted BoNT injections were non-inferior, terms to current injection practices. Design Open-label prospective evaluator-blinded study. Setting Conducted across 20 medical centres Denmark, Finland, Norway and Sweden (24 September 2012 11 March...
<b><i>Background:</i></b> Small intestinal neuroendocrine tumors (SI-NETs) are difficult to diagnose in the early stage of disease. Current blood biomarkers such as chromogranin A (CgA) and 5-hydroxyindolacetic acid have low sensitivity (SEN) specificity (SPE). This is a first preplanned interim analysis (Nordic non-interventional, prospective, exploratory, EXPLAIN study [NCT02630654]). Its objective investigate if plasma protein multi-biomarker strategy can improve...
Locally formed arachidonic acid (AA) metabolites are important as modulators of many aspects renal tubular function, including regulation the activity Na + -K -ATPase. Here we examined ontogeny AA metabolic pathways regulating proximal convoluted (PCT) -ATPase in infant and adult rats. Eicosatetraynoic acid, an inhibitor all AA-metabolizing pathways, abolished this effect. inhibition PCT was blocked by 12-lipoxygenase baicalein but not rats specific cytochrome P-450 fatty ω-hydroxylase...
Abstract There is an unmet need for novel biomarkers to diagnose and monitor patients with neuroendocrine neoplasms. The EXPLAIN study explores a multi‐plasma protein supervised machine learning strategy improve the diagnosis of pancreatic tumors (PanNET) differentiate them from small intestinal (SI‐NET). At time diagnosis, blood samples were collected analyzed 39 PanNET, 135 SI‐NET (World Health Organization Grade 1–2) 144 controls. Exclusion criteria other malignant diseases, chronic...
Current selection markers allow by antibiotics or fluorescent/magnetic sorting green fluorescent protein membrane antigens. Antibiotic proceeds on a time scale of weeks, and flourescence-activated cell requires complex equipment may generate false-positive results when is performed too early after transduction with markers. We have characterized an endogenous eukaryotic marker, the ouabain resistance gene (Ouar), which has potential for quick efficient in vitro target cells. The Ouar used us...