A5100397791- CRISPR and Genetic Engineering
- Plant Gene Expression Analysis
- Photosynthetic Processes and Mechanisms
- RNA and protein synthesis mechanisms
- Plant biochemistry and biosynthesis
- Advanced biosensing and bioanalysis techniques
- Plant Virus Research Studies
- Pluripotent Stem Cells Research
- Plant Molecular Biology Research
- RNA Interference and Gene Delivery
- Research in Cotton Cultivation
- Plant Stress Responses and Tolerance
- RNA regulation and disease
- Remote Sensing in Agriculture
- Animal Genetics and Reproduction
- Insect Resistance and Genetics
- Plant Reproductive Biology
- Microbial Natural Products and Biosynthesis
- Phytochemicals and Antioxidant Activities
- Remote Sensing and LiDAR Applications
- Fungal Biology and Applications
- Chromosomal and Genetic Variations
- Viral Infections and Immunology Research
- Lipid metabolism and biosynthesis
- Insect symbiosis and bacterial influences
Huazhong Agricultural University
2014-2025
Southeast University
2025
Guangxi Medical University
2023-2024
Yunnan Academy of Agricultural Sciences
2021-2024
Ministry of Agriculture and Rural Affairs
2021-2024
Yangtze River Pharmaceutical Group (China)
2024
Huashan Hospital
2024
Fudan University
2024
Chongqing Academy of Agricultural Sciences
2014-2024
Institute of Vegetables and Flowers
2024
Abstract Yellow seed is a desirable trait with great potential for improving quality in Brassica crops. Unfortunately, no natural or induced yellow germplasms have been found napus , an important oil crop, which likely reflects its genome complexity and the difficulty of simultaneous random mutagenesis multiple gene copies functional redundancy. Here, we demonstrate first application CRISPR/Cas9 creating yellow‐seeded mutants rapeseed. The targeted mutations BnTT8 were stably transmitted to...
Summary Multilocular silique is a desirable agricultural trait with great potential for the development of high‐yield varieties Brassica . To date, no spontaneous or induced multilocular mutants have been reported in napus , which likely reflects its allotetraploid nature and extremely low probability simultaneous random mutagenesis multiple gene copies functional redundancy. Here, we present evidence efficient knockout rapeseed homologues CLAVATA 3 ( CLV ) secreted peptide related receptors...
Urokinase plasminogen activator receptor (uPAR), a member of the lymphocyte antigen 6 protein superfamily, is overexpressed in different types cancers and plays an important role tumorigenesis development. In this study, we successfully targeted uPAR by CRISPR/Cas9 system two human cancer cell lines with individual sgRNAs. Knockout inhibited proliferation, migration invasion. Furthermore, knockout decreases resistance to 5-FU, cisplatin, docetaxel, doxorubicin these cells. Although there are...
Tumorous stem mustard (Brassica juncea var. tumida Tsen et Lee) is an economically and nutritionally important dietary vegetable in Asian countries. Purple tumorous contains inflated abundant anthocyanin accumulation leaves. Here, 20 anthocyanins were separated identified from the purple by high-performance liquid chromatography–electrospray ionization–tandem mass spectrometry (HPLC–ESI–MS/MS). In order to investigate regulatory production mustard, expression of biosynthetic genes leaves...
Galactinol synthase (GolS; EC 2.4.1.123) is a member of the glycosyltransferase eight family that catalyzes first step in biosynthesis pathway raffinose oligosaccharides (RFOs). The accumulation RFOs response to abiotic stress indicates role for adaptation. To obtain information on roles adaptation trees, we investigated expression patterns nine Populus trichocarpa GolS (PtrGolS) genes with special reference responses. PtrGolS were differentially expressed different organs, and expressions...
Bee pollen has been regarded as a complete nutritional human dietary supplement but its nutrient absorption and biological effects may be restricted by the complex wall. The aim of this study was to explore ultrasonic ball-milling treatment on release components in vitro vivo antioxidant rose (Rosa rugosa) bee pollen.Bee walls were broken varying degrees, nutrients released, improved. scavenging 1,1-diphenyl-2-picrylhydrazyl (DPPH) 2,2'-azinobis(3-ethylbenzothiazolone-6-sulfonic acid) (ABTS)...
We explored whether the Clustered regularly interspaced short palindromic repeat (CRISPR)-Cas and restriction-modification (R-M) systems are compatible act together to resist plasmid attacks.
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) is an adaptive immune system that resists foreign genes through nuclease targeting in bacteria and archaea. In this study, we analyzed 68 strains of Lactobacillus casei group from the NCBI GenBank database, bioinformatic tools were used to investigate occurrence diversity CRISPR system. The results showed a total 30 loci identified 27 strains. Apart three which contained double with distinguishable distributed sites, most...
In recent years, unmanned aerial vehicle (UAV) remote sensing systems have advanced rapidly, enabling the effective assessment of crop growth through processing and integration multimodal data from diverse sensors mounted on UAVs. UAV-derived encompass both multi-source non-remote data. This study employs Image Guided Filtering Fusion (GFF) to obtain high-resolution multispectral images (HR-MSs) selects three vegetation indices (VIs) based correlation analysis feature reduction in HR-MS for...
Abstract Citric acid (CA) plays a crucial role as fruit flavor enhancer and serves mediator in multiple metabolic pathways tomato development. Understanding factors influencing CA metabolism is essential for enhancing CA-mediated biological processes. The accumulation of CA, however, influenced by complex interplay genetic environmental factors, leading to challenges accurately predicting regulating its levels. In this study, we conducted genome-wide association study (GWAS) on employing six...
Trametes versicolor is an important fungus with medicinal properties and a significant role in lignocellulose degradation. In this study, we constructed high-quality chromosome-level genome of T. using Illumina, PacBio HiFi, Hi-C sequencing technologies. The assembled 47.42 Mb size contains 13,307 protein-coding genes. BUSCO analysis revealed gene completeness results 95.80% 95.90%, respectively. Phylogenetic showed that most closely related to pubescens, followed by cinnabarina coccinea....
Digital mental health interventions, including AI-integrated applications, are increasingly utilized to support individuals with elevated symptoms of psychological distress. However, a gap exists in understanding their efficacy specifically for student populations. This study aimed investigate the effects GymBuddy, an AI-powered fitness and accountability app, Elomia, AI-based chatbot, on students at risk A quasi-experimental was conducted involving 65 participants who exhibited heightened...
Grifola frondosa, commonly known as turkey tail, is a valuable fungus with medicinal and culinary uses, rich in bioactive compounds like triterpenoid polysaccharides that contribute to health benefits. Here, we constructed nearly complete genome of G. frondosa CH1 using Illumina, PacBio HiFi, Hi-C sequencing technologies, resulting 35.74 Mb 12,526 protein-coding genes. The spans 12 chromosomes, all intact telomeric structures no gaps. BUSCO completeness scores 95.1% 99.1% for the genes,...
Sex determination has been studied in the model lepidopteran species Bombyx mori, but it remains poorly understood pests. In present study, we identified and characterized Masculinizer (Masc) gene a Noctuidae pest species, Agrotis ipsilon. Sequence analysis revealed that AiMasc encodes protein of 658 amino acids two CCCH-type zinc finger domains conserved cysteine residues (Cys-277 Cys-280). We assessed masculinizing activity BmN cells found induced expression male-specific doublesex...
Superoxide dismutase (SOD) as a group of significant and ubiquitous enzymes plays critical function in plant growth development. Previously this gene family has been investigated Arabidopsis rice; it not yet characterized cotton. In our study, was the first time for us to perform genome-wide analysis SOD Our results showed that 10 genes were identified Gossypium arboreum raimondii , including 6 Cu-Zn-SODs, 2 Fe-SODs, Mn-SODs. The chromosomal distribution revealed are distributed across 7...
Tyrosine hydroxylase (TH) is involved in insect melanin and the catecholamine biosynthesis pathway. TH as an enzyme catalyzing conversion of tyrosine to 3,4-dihydroxyphenylalanine first step reaction Although has been proven affect pigmentation epidermis development many insects, there no report about physiological function gene Agrotis ipsilon. Here we cloned from A. Semi-quantitative real-time polymerase chain (PCR) analysis showed that AiTH was expressed at all stages. Moreover, its high...