A5040055491- Photosynthetic Processes and Mechanisms
- CRISPR and Genetic Engineering
- Nitrogen and Sulfur Effects on Brassica
- Chromosomal and Genetic Variations
- Plant nutrient uptake and metabolism
- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant biochemistry and biosynthesis
- Lipid metabolism and biosynthesis
- Plant tissue culture and regeneration
- Forest ecology and management
- Plant-Microbe Interactions and Immunity
- Viral Infections and Vectors
- Environmental Conservation and Management
- Plant Gene Expression Analysis
- Forest Ecology and Biodiversity Studies
- Antioxidant Activity and Oxidative Stress
- Plant Disease Resistance and Genetics
- Vector-borne infectious diseases
- Vector-Borne Animal Diseases
- Plant Pathogens and Resistance
- RNA regulation and disease
- RNA and protein synthesis mechanisms
- Genetic Mapping and Diversity in Plants and Animals
Huazhong Agricultural University
2019-2023
Shanghai Zhangjiang Laboratory
2022
Abstract Yellow seed is a desirable trait with great potential for improving quality in Brassica crops. Unfortunately, no natural or induced yellow germplasms have been found napus , an important oil crop, which likely reflects its genome complexity and the difficulty of simultaneous random mutagenesis multiple gene copies functional redundancy. Here, we demonstrate first application CRISPR/Cas9 creating yellow‐seeded mutants rapeseed. The targeted mutations BnTT8 were stably transmitted to...
Abstract Seed size and number are central to the evolutionary fitness of plants also crucial for seed production crops. However, molecular mechanisms control poorly understood in Brassica Here, we report gene cloning, expression analysis, functional characterization EOD3/CYP78A6 rapeseed. BnaEOD3 has four copies located two subgenomes, which exhibited a steady higher during development with differential among copies. The targeted mutations were efficiently generated by stable transformation...
Manipulation of flowering time to develop cultivars with desired maturity dates is fundamental in plant breeding. It desirable generate polyploid rapeseed (Brassica napus L.) germplasm varying controlled by a few genes. In the present study, BnaSVP, homolog Arabidopsis SVP (Short Vegetative Phase) gene, was characterized and set mutants developed using CRISPR/Cas9-based gene-editing tool. A single construct targeting multiple sites successfully applied precisely mutate four copies BnaSVP....
The diversity of petal and leaf color can improve the ornamental value rapeseed promote development agriculture tourism. two copies carotenoid isomerase gene ( BnaCRTISO ) in Brassica napus BnaA09.CRTISO BnaC08.CRTISO was edited using CRISPR/Cas9 system present study. mutation phenotype creamy white petals yellowish leaves could be recovered only targeted mutants both functional copies, indicating that redundant roles are vital for regulation color. content double mutant significantly...
Abstract The CLE ( CLAVATA3 / ESR ) gene family, encoding a group of small secretory peptides, plays important roles in cell‐to‐cell communication, thereby controlling broad spectrum development processes. family has been systematically characterized some plants, but not Brassica napus . In the present study, 116 BnCLE genes were identified B. genome, including seven unannotated, six incorrectly predicted and five multi‐CLE domain‐encoding genes. These members separated into distinct groups...
CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) technology has revolutionized plant pathology by providing a precise and efficient tool for enhancing disease resistance in crops. This gene-editing technique enables scientists to modify specific DNA sequences plants, allowing the targeted improvement of traits related pathogen defense. By altering genes responsible susceptibility diseases, facilitates development crops that are more resistant bacterial, fungal, viral...
Rapeseed is one of the world's most important sources oilseed crops. Single nucleotide substitution basis genetic variation underpinning agronomic traits. Therefore, genome-wide and target-specific base editing will greatly facilitate precision plant molecular breeding. In this study, four CBE systems (BnPBE, BnA3A-PBE, BnA3A1-PBE, BnPBGE14) were modified to achieve cytidine at five target genes in rapeseed. The results indicated that genome achievable three CBEs systems, among which...
Abstract Key message A major QTL controlling ovule abortion and SN was fine-mapped to a 80.1-kb region on A8 in rapeseed, BnaA08g07940D BnaA08g07950D are the most likely candidate genes. Abstact The seed number per silique (SN), an important yield determining trait of is final consequence complex developmental process including initiation subsequent ovule/seed development. To elucidate genetic mechanism regulating natural variation its related components, quantitative locus (QTL) mapping...
The Arabidopsis homeodomain transcription factor SHOOT MERISTEMLESS ( STM ) is crucial for shoot apical meristem (SAM) function, which cooperates with CLAVATA3 CLV3 /WUSCHEL WUS feedback regulation loops to maintain the homeostasis of stem cells in SAM. also interacts boundary genes regulate tissue formation. However, there are still few studies on function Brassica napus , an important oil crop. There two homologs B. BnaA09g13310D and BnaC09g13580D ). In present study, CRISPR/Cas9...