A5052334024- Plasmonic and Surface Plasmon Research
- Near-Field Optical Microscopy
- Advanced Fluorescence Microscopy Techniques
- Gold and Silver Nanoparticles Synthesis and Applications
- Extracellular vesicles in disease
- Advanced biosensing and bioanalysis techniques
- Advanced Biosensing Techniques and Applications
- Optical Coatings and Gratings
- Photonic and Optical Devices
- Photoacoustic and Ultrasonic Imaging
- Integrated Circuits and Semiconductor Failure Analysis
- MicroRNA in disease regulation
- Quantum Dots Synthesis And Properties
- Cell Image Analysis Techniques
- Optical Coherence Tomography Applications
- Biosensors and Analytical Detection
- Photonic Crystals and Applications
- Nanopore and Nanochannel Transport Studies
- Digital Imaging for Blood Diseases
- Neuroscience and Neuropharmacology Research
- Cardiac Imaging and Diagnostics
- Advanced Nanomaterials in Catalysis
- Antiplatelet Therapy and Cardiovascular Diseases
- Coronary Interventions and Diagnostics
- Digital Holography and Microscopy
Center for Systems Biology
2020-2023
Yonsei University
2014-2023
Massachusetts General Hospital
2020-2023
Imperial College London
1968
MicroRNAs (miRNAs) in extracellular vesicles (EVs) play essential roles cancer initiation and progression. Quantitative measurements of EV miRNAs are critical for diagnosis longitudinal monitoring. Traditional PCR-based methods, however, require multi-step procedures remain as bulk analysis. Here, the authors introduce an amplification-free extraction-free miRNA detection method using a CRISPR/Cas13a sensing system. components encapsulated liposomes delivered them into EVs through...
Abstract Cholangiocarcinoma (CCA) is a fatal disease often detected late in unresectable stages. Currently, there are no effective diagnostic methods or biomarkers to detect CCA early with high confidence. Analysis of tumor‐derived extracellular vesicles (tEVs) harvested from liquid biopsies can provide new opportunity achieve this goal. Here, an advanced nanoplasmonic sensing technology reported, termed FLEX (fluorescence‐amplified vesicle technology), for sensitive and robust single EV...
Abstract Extracellular vesicles (EVs)—nanoscale phospholipid secreted by cells—present new opportunities for molecular diagnosis from non‐invasive liquid biopsies. Single EV protein analysis can be extremely valuable in studying EVs as circulating cancer biomarkers, but it is technically challenging due to weak detection signals associated with limited amounts of epitopes and small surface areas antibody labeling. Here, a new, simple method that enables multiplexed analyses markers improved...
We investigated the transport of neuronal mitochondria using superlocalized near-fields with plasmonic nanohole arrays (PNAs). Compared to traditional imaging techniques, PNAs create a massive array light beams and allow 3D mitochondrial dynamics be sampled extracted almost in real time. In this work, fluorescence excited by was captured an optical microscope dual objective lenses, which produced while minimizing scattering substrate. It found that move average velocity 0.33 ± 0.26 μm/s,...
We demonstrate the surface-plasmon-induced enhancement of Förster resonance energy transfer (FRET)using a model multilayer core-shell nanostructure consisting an Au core and surrounding FRET pairs, i.e., CdSe quantum dot donors S101 dye acceptors. The configuration was demonstrated to exhibit synergistic effects surface plasmon from metal plasmon-enhanced dots dye. With precise control over distance between components in nanostructure, significant improvement emission achieved by combined...
A deep learning approach has been taken to improve detection characteristics of surface plasmon microscopy (SPM) light scattering. Deep based on the convolutional neural network algorithm was used estimate effect scattering parameters, mainly number scatterers. The improvement assessed a quantitative basis by applying SPM images formed coherent interference It found that significantly improves accuracy over conventional detection: enhancement in shown be higher almost 6 times and useful for...
Polymerase chain reaction (PCR)-based diagnostic kits for point-of-care (POC) testing are highly desirable to prevent the spread of infectious diseases. Here, we demonstrate a rapid PCR kit that involves integrating lateral flow paper strip with nichrome-based thin film heater. The use membrane as PCR-solution container results in fast thermocycling without cooler because can contain solution high specific surface area where Joule heating is applied. After PCR, amplified products...
The feasibility of super-resolution microscopy has been investigated based on random localization surface plasmon using blocked nanodot arrays. resolution is mainly determined by the size localized fields in range 100-150 nm. concept was validated imaging FITC-conjugated phalloidin that binds to cellular actin filaments. experimental results confirm improved reconstructed images. Effect far-field registration image reconstruction also analyzed. Correlation between images maintained be above...
In this Letter, we describe spatially switched surface plasmon microscopy (ssSPM) based on two-channel momentum sampling. The performance evaluated with periodic nanowires in comparison conventional SPM and bright-field shows that the resolution of ssSPM is enhanced by almost 15 times over SPM. provides an extremely simple way to attain diffraction limit go beyond for super-resolution label-free techniques.
The incorporation of plasmonic nanoparticles (NPs) into photovoltaic devices can increase light absorption and in turn improve solar cell performance. graphene oxide-encapsulated gold NPs (Au@GO NPs) are designed incorporated photoanodes to demonstrate dye-sensitized cells. coupling between GO the Au has beneficial effect extending spectral utilization long-wavelength portion visible spectrum. In addition, encapsulation reduces charge recombination on surface facilitates improved transport....
Abstract In this study, we have investigated localization-based microscopy to achieve full-field super-resolution. For localized sampling, considered combs consisting of unit pulses and near-fields by surface nanoapertures. Achievable images after reconstruction were assessed in terms peak signal-to-noise ratio (PSNR). It was found that spatial switching individual may be needed break the diffraction limit. Among parameters, resolution largely determined sampling period while effect width a...
Plasmonic biosensors are increasingly being used for the analysis of extracellular vesicles (EVs) originating from disease areas. However, high non-specific binding EVs to a gold-sensing surface has been critical problem and hindered true translational potential. Here, we report that direct antibody immobilization on plasmonic gold via physisorption shows excellent capture cancer-derived with ultralow even at very concentrations. Contrary commonly methods involve thiol-based linker...
Abstract Random nanocomposite islands have drawn significant interest for diverse applications due to extreme simplicity of fabricating nanoscale structures. In this paper, the near‐field characteristics random nanoislands produced by spatial light switching are investigated, with incidence among 14 incident channel modes. The results show that localized fields displaced from almost linearly angle channels. Also, rotate in a manner aligned direction incidence. Ten‐channel is found provide...
We present a theoretical approach to single nanoparticle detection using surface plasmon scattering microscopy. Through rigorous coupled wave analysis assuming light incidence on gold coated BK7 glass substrate under total internal reflection condition for 200-nm polystyrene as targets attached the film, it was found that polariton induced by incident thin film is perturbed. As result, parabolic waves were observed in plane. By varying angles of and wavelengths, optimum conditions microscopy...
Abstract Near-field optics is essential in many nanotechnology applications, such as implementing sensitive biosensing and imaging systems with extreme precision. Understanding optical near-fields at the nanoscale has so attracted considerable research interest, which use a variety of analytical approaches, most notably near-field scanning microscopy. Here, we show defocused point localization mapped accumulation (DePLOMA), can overcome weaknesses conventional methods. DePLOMA based on...
Localized surface plasmon enhanced microscopy based on nanoislands of random spatial distribution was demonstrated for imaging live cells and molecular interactions. Nanoislands were produced without lithography by high temperature annealing under various processing conditions. The localization near-field that is associated with localized metallic analyzed theoretically experimentally in comparison periodic nanostructures. For experimental validation cell imaging, mouse macrophage-like line...
Surface-plasmon enhanced microscopy has been investigated using blocked random nanodot arrays. J744 cells were imaged on arrays under total internal reflection. The images deconvolved based localized near-field distribution for super-resolution. Experimentally achieved resolution is estimated to be 100-150 nm. Effects of signal-to-noise and registration the reconstructed are discussed.