- Algal biology and biofuel production
- Photosynthetic Processes and Mechanisms
- Glycosylation and Glycoproteins Research
- T-cell and B-cell Immunology
- Aquatic Ecosystems and Phytoplankton Dynamics
- Lipid metabolism and biosynthesis
- Monoclonal and Polyclonal Antibodies Research
Shanghai Jiao Tong University
2023-2025
Henan Academy of Agricultural Sciences
2011
Alkaline environments such as alkaline lands, lakes, and industrial wastewater are not conducive to the growth of plants microorganisms due high pH salinity. ChbZIP1 is a bZIP family transcription factor isolated from an alkaliphilic microalgae (Chlorella sp. BLD). Previous studies have demonstrated its ability enhance tolerance in Arabidopsis thaliana. However, potential confer similar other remains unclear, specific mechanisms fully understood. The analysis cellular physiological...
The properties and nutritional value of microalgal bioproducts depend significantly on fatty acid desaturation, which is generally modulated by manipulating the culture conditions or associated gene expressions. Here, we investigated role CpZF_CCCH1, a non-tandem CCCH-type zinc-finger (non-TZF) protein, in elevating polyunsaturated (PUFA) content (11.00-16.36%) Chlamydomonas reinhardtii. Through lipidomic flow cytometry analyses, observed reduced triacylglycerol accumulation (7.01-21.15%)...
In order to further the study properties of mouse Fc gamma receptor III (moFcγRIII) and γ chain their genes were co-expressed in cultured COS7 cell a stable line was produced. The moFcγRIII isolated from peripheral blood leucocyte mRNA, amplified separately by RT-PCR, eukaryotic expression plasmids pc3mRIII pc3mγ constructed. After identification restriction digestion PCR, recombinant Pvu I-linearised co-transfected into cells with lipofectamine. Cloning limiting dilution after selective...