A deficiency of plasmalogens, caused by impaired peroxisomal metabolism affects normal development and multiple organs in adulthood. Treatment options aimed at restoring plasmalogen levels may be relevant for the therapy non-peroxisomal disorders. In this study we determined vivo efficacy an alkyl glycerol (AG), namely, 1-O-octadecyl-rac-glycerol, as a therapeutic agent defects synthesis. To achieve this, Pex7 knockout mice, mouse model Rhizomelic Chondrodysplasia Punctata type 1...

The genus Burkholderia includes strains pathogenic to animals and plants, bioremediators, or plant growth promoters. Genome sequence analyses of representative cepacia complex (Bcc) non-Bcc for the presence bce-I gene cluster, directing biosynthesis exopolysaccharide (EPS) cepacian, further extended this previously described cluster by another 9 genes. genes in bce-II were named bceM bceU encode products putatively involved nucleotide sugar precursor repeat unit assembly, modification,...

Biofilms are communities of microbial cells that encapsulated within a self-produced polymeric matrix. The matrix is critical to the success biofilms in diverse habitats; however, many details composition, structure, and function remain enigmatic. formed by Gram-positive bacterium Bacillus subtilis depend on production secreted film-forming protein BslA. Here, we show gradient electron acceptor availability through depth biofilm gives rise two distinct functional roles for BslA these can be...

The biosynthesis of the exopolysaccharide (EPS) cepacian by Burkholderia cepacia complex strains requires 16.2-kb bce cluster genes. Two clustered genes, bceD and bceF, code for two proteins homologous to phosphotyrosine phosphatases tyrosine kinases, respectively. We show experimental evidence indicating that BceF is phosphorylated on conserved lysine residue present at position 563 in Walker A ATP-binding motif required this autophosphorylation. It was also proved BceD capable...

Burkholderia cepacia complex (Bcc) bacteria are opportunistic pathogens infecting hosts such as cystic fibrosis (CF) patients. Long-term Bcc infection of CF patients' airways has been associated with emergence phenotypic variation. Here we studied two multivorans clonal isolates displaying different morphotypes from a chronically infected patient to evaluate trait development during lung infection. Expression profiling mucoid D2095 and non-mucoid D2214 revealed decreased expression genes...

ABSTRACT The bacterial tyrosine-kinase (BY-kinase) family comprises the major group of enzymes endowed with tyrosine kinase activity. We previously showed that BceF protein from Burkholderia cepacia IST408 belongs to this BY-kinase and is involved in biosynthesis exopolysaccharide cepacian. However, little known about extent regulation In order examine regulation, we performed a comparative transcriptome profile between bceF mutant wild-type B. IST408. analyses led identification 630 genes...

Abstract BslA is a protein secreted by Bacillus subtilis which forms hydrophobic film that coats the biofilm surface and renders it water-repellent. We have characterised three orthologues of from amyloliquefaciens , licheniformis pumilus as well paralogue B . called YweA. find orthologous proteins can substitute for in confer degree protection, whereas YweA cannot. The to functionally native correlates with their vitro biophysical properties. Our results demonstrate use naturally-evolved...

The soybean (Glycine max (L.) Merr.) is a crop mainly grown under rain fed conditions although irrigation increasingly being used. Water deficiency the main factor limiting seed production. symbiosis process also negatively affected by water stress. isoflavone genistein have been recognized as powerful inducer of Nod factors production Bradyrhizobium and its addition to inocula has shown increase nodule number promote nitrogen (N) fixation at low temperatures. This study looks for answers...

To compare the virulence pool and acute infection ability of Pseudomonas aeruginosa isolates from a hydropathic facility, used to treat respiratory conditions by inhalation untreated natural mineral water, with clinical infections.Pseudomonas facility infections were typed pulsed-field gel electrophoresis. Nonclonal representatives each population selected. 18 virulence-encoding genes screened polymerase chain reaction statistically compared multiple correspondence analysis. Homogeneous...

Burkholderia cepacia complex (Bcc) bacteria are opportunistic pathogens causing chronic respiratory infections particularly among cystic fibrosis patients. During these infections, mucoid-to-nonmucoid morphotype variation occurs, with the two morphotypes exhibiting different phenotypic properties. Here we show that in vitro, mucoid clinical isolate multivorans D2095 gives rise to stable nonmucoid variants response prolonged stationary phase, presence of antibiotics, and osmotic oxidative...

Bacterial tyrosine kinases and their cognate protein phosphatases are best known for regulating the biosynthesis of polysaccharides. Moreover, roles in stress response, DNA metabolism, cell division, virulence have also been documented. The aim this study was to investigate pathogenicity potential mechanisms dependent on kinase BceF phosphotyrosine phosphatase BceD cystic fibrosis opportunistic pathogen Burkholderia contaminans IST408. insertion mutants bceD::Tp bceF::Tp showed similar...

A new subtype of archaeal Rieske ferredoxin (RFd) has been identified in the genome thermoacidophilic archaeon Acidianus ambivalens . The gene is inserted an atypical genomic context a cluster encoding NiFe hydrogenase. Sequence and phyletic analysis showed that protein related to bacterial RFd but not any known proteins. recombinant 14 kDa isolated from Escherichia coli behaved as dimer solution. It contained ∼2 Fe/mol all visible EPR spectroscopic features typical centre‐containing...

Abstract Biofilms are communities of microbial cells that encapsulated within a self-produced polymeric matrix. The matrix is critical to the success biofilms in diverse habitats, but despite this many details composition, structure, and function remain enigmatic. formed by Gram-positive bacterium Bacillus subtilis depend on production secreted film-forming protein BslA. Here we show gradient electron acceptor availability through depth biofilm gives rise two distinct functional roles for...

Introdução: Estudos destacam que o trabalho é essencial na vida tanto de homens quanto mulheres, no entanto, quando exercido em situações inadequadas, pode ser prejudicial à saúde. Certos grupos trabalhadores, devido às suas características ocupacionais, tornam-se mais suscetíveis ao desenvolvimento dores musculoesqueléticas relacionadas trabalho. Esses distúrbios afetam uma ampla gama ocupações e são reconhecidos como das principais causas incapacidade relacionada Objetivo: Analisar a...

Abstract BslA is a protein secreted by Bacillus subtilis which forms hydrophobic film that coats the biofilm surface and renders it water-repellent. We have characterised three orthologues of from amyloliquefaciens , licheniformis pumilus as well paralogue B. called YweA. find orthologous proteins can substitute for in confer degree protection, whereas YweA cannot. The to functionally native correlates with their vitro biophysical properties. Our results demonstrate use naturally-evolved...

Comparative and descriptive analysis of the outcomes percutaneous access for EVAR (pEVAR) compared to control group submitted surgical femoral (cEVAR). Retrospective case–control between January 2013 2015 pEVAR (group 1) a cEVAR 2) taking into account demographic data primary endpoints: Access complication time; secondary type anaesthesia, length hospital stay hematic loss. The statistical was performed with SPSS 23.0 programme using X2 test categorical variables t continuous variables. A...

Burkholderia cepacia complex bacteria are opportunistic pathogens, causing severe lung infections in cystic fibrosis patients. Many clinical isolates produce the exopolysaccharide cepacian, believed to be an infection persistence determinant. Little is known about cepacian biosynthesis regulation, but our studies showed importance of post-translational mechanisms mediated by tyrosine phosphorylation, namely through autokinase BceF, which affects EPS biosynthesis. Indeed, insertion mutants on...