A5027089325- Hepatitis C virus research
- RNA and protein synthesis mechanisms
- Hepatitis B Virus Studies
- Cytomegalovirus and herpesvirus research
- CRISPR and Genetic Engineering
- Immune Cell Function and Interaction
- Genomics and Chromatin Dynamics
- Hepatitis Viruses Studies and Epidemiology
- RNA Research and Splicing
- Bacteriophages and microbial interactions
- Epigenetics and DNA Methylation
- Liver Disease Diagnosis and Treatment
- Immunodeficiency and Autoimmune Disorders
- Virus-based gene therapy research
- Genomics and Phylogenetic Studies
- RNA modifications and cancer
- Respiratory viral infections research
- Innovation and Socioeconomic Development
- T-cell and B-cell Immunology
- Animal Virus Infections Studies
- Genetic Associations and Epidemiology
- Inflammasome and immune disorders
- Psoriasis: Treatment and Pathogenesis
- HIV Research and Treatment
MRC Human Immunology Unit
2020-2023
University of Oxford
2018-2023
MRC Weatherall Institute of Molecular Medicine
2018-2023
Medical Research Council
2020
National Institute for Health Research
2020
Heidelberg University
2019
University Hospital Heidelberg
2019
Abstract Chromosome conformation capture (3C) provides an adaptable tool for studying diverse biological questions. Current 3C methods generally provide either low-resolution interaction profiles across the entire genome, or high-resolution at limited numbers of loci. Due to technical limitations, generation reproducible has not been achieved genome-wide scale. Here, overcome this barrier, we systematically test each step and report two improvements over current methods. We show that up 30%...
Abstract Hepatitis delta virus (HDV) depends on the helper function of hepatitis B (HBV), which provides envelope proteins for progeny secretion. Current infection-competent cell culture models do not support assembly and secretion HDV. By stably transducing HepG2 cells with genes encoding NTCP-receptor HBV we produce a line (HepNB2.7) that allows continuous infectious HDV following primary infection. Evaluation antiviral drugs shows entry inhibitor Myrcludex (IC 50 : 1.4 nM) interferon-α 28...
Group A
ABSTRACT Genome-wide association studies (GWAS) have identified over 150,000 links between common genetic variants and human traits or complex diseases. Over 80% of these associations map to polymorphisms in non-coding DNA. Therefore, the challenge is identify disease-causing variants, genes they affect, cells which effects occur. We developed a platform using ATAC-seq, DNaseI footprints, NG Capture-C machine learning address this challenge. Applying approach red blood cell identifies...
The emergence in recent years of DNA editing technologies-Zinc finger nucleases (ZFNs), transcription activator-like effector (TALE) guided (TALENs), clustered regularly interspaced short palindromic repeats (CRISPR)/Cas family enzymes, and Base-Editors-have greatly increased our ability to generate hundreds edited cells carrying an array alleles, including single-nucleotide substitutions. However, the infrequency homology-dependent repair (HDR) generating these substitutions general...
ABSTRACT Chromosome conformation capture (3C) provides an adaptable tool for studying diverse biological questions. Current 3C methods provide either low-resolution interaction profiles across the entire genome, or high-resolution at up to several hundred loci. All are affected varying degrees by inefficiency, bias and noise. As such, generation of reproducible has not been achieved scale. To overcome this barrier, we systematically tested improved upon current methods. We show that...
Abstract Retroviral restriction factors are important regulators of viral infection, targeting vulnerable steps the virus lifecycle; that also targeted by antiviral drugs. It has become clear route cellular infection can alter sensitivity HIV-1 to these agents. Using CRISPR-Cas9 edited pluripotent stem cell-derived macrophages, we have explored potential a modified factor (human TRIMCyp) inhibit replication in both cell free and cell-cell models. We show expression TRIMCyp from endogenous...