A5048119379- Endoplasmic Reticulum Stress and Disease
- Genetics, Aging, and Longevity in Model Organisms
- Microtubule and mitosis dynamics
- Genomics and Chromatin Dynamics
- RNA Interference and Gene Delivery
- Single-cell and spatial transcriptomics
- Plant Genetic and Mutation Studies
- Nuclear and radioactivity studies
- Heat shock proteins research
- Cellular and Composite Structures
- Chromosomal and Genetic Variations
- Plant Molecular Biology Research
- interferon and immune responses
- Pluripotent Stem Cells Research
- Plant Reproductive Biology
- Virus-based gene therapy research
- CRISPR and Genetic Engineering
- Mechanical Failure Analysis and Simulation
Ludwig-Maximilians-Universität München
2024
Nantes Université
2023
Inserm
2023
Translational Research in Gene Therapy
2023
ETH Zurich
2017
Abstract Direct neuronal reprogramming is a promising approach to regenerate neurons from local glial cells. However, mechanisms of epigenome remodeling and co-factors facilitating this process are unclear. In study, we combined single-cell multiomics with genome-wide profiling three-dimensional nuclear architecture DNA methylation in mouse astrocyte-to-neuron mediated by Neurogenin2 (Ngn2) its phosphorylation-resistant form (PmutNgn2), respectively. We show that Ngn2 drives multilayered...
Recombinant Adeno-Associated Virus (rAAV) is considered as one of the most successful and widely used viral vectors for in vivo gene therapy. However, host immune responses to vector and/or transgene product remain a major hurdle AAV transfer. In contrast antivector adaptive immunity, initiation innate immunity towards rAAV still poorly understood but directly dependent on interaction between cells. Here, we quantitative transcriptomic-based approach determine activation inflammatory...
Numerous target genes of the Polycomb group (PcG) are transiently activated by a stimulus and subsequently repressed. However, mechanisms which PcG proteins regulate such remain elusive. We employed heat shock-responsive hsp70 locus in Drosophila to study chromatin dynamics PRC1 its interplay with known regulators before, during after shock. detected mutually exclusive binding patterns for HSF at locus. found that Pleiohomeotic (Pho), DNA-binding member, dynamically interacts Spt5, an...