Automated machine learning (AutoML) has been recognized as a powerful tool to build system that automates the design and optimizes model selection (ML) pipelines. In this study, we present tree-based pipeline optimization (TPOT) method for determining ML models with significant performance less complex breast cancer diagnostic Some features of pre-processors are defined expression trees optimal gene programming (GP) pipelines, stochastic search system. Features radiomics have presented guide...

Abstract Background Vitamin E supplements containing tocotrienols are now being recommended for optimum health but its effects scarcely known. The objective was to determine the of Tocotrienol Rich Fraction (TRF) supplementation on lipid profile and oxidative status in healthy older individuals at a dose 160 mg/day 6 months. Methods Sixty-two subjects were recruited from two age groups: 35-49 years (n = 31) above 50 31), randomly assigned receive either TRF or placebo capsules six Blood...

Background High-dose synchrotron microbeam radiation therapy (MRT) has shown the potential to deliver improved outcomes over conventional broadbeam (BB) therapy. To implement MRT clinically for cancer treatment, it is necessary undertake dose equivalence studies identify doses that give similar BB treatments. Aim develop an in vitro approach determine biological between and using two different cell-based assays. Methods The acute response of tumour normal cell lines (EMT6.5, 4T1.2, NMuMG,...

To investigate the stability of seven housekeeping genes: beta-actin (

Lapatinib (LAP), a dual inhibitor targeting ErbB1 and ErbB2 tyrosine kinases, demonstrates efficacy in treating ErbB2-positive breast cancer. However, its clinical use is hindered by the occurrence of associated diarrhoea. expressed intestine; leading to hypothesis that lapatinib inhibits normal function, causing This study explores potential role inhibition mechanism lapatinib-induced Caco-2 intestinal monolayers were treated with LAP alone combination recombinant epidermal growth factor...

Hepatocellular carcinoma (HCC) is considered as a complex liver disease and ranked the eighth-highest mortality rate with prevalence of 2.4% in Malaysia. Magnetic resonance imaging (MRI) has been acknowledged for its advantages, gold technique diagnosing HCC, yet false-negative diagnosis from examinations inevitable. In this study, 30 MR images patients diagnosed HCC used to evaluate robustness semi-automatic segmentation using flood fill algorithm quantitative features extraction. The...

The aim of this study was to identify genes and molecular pathways differentially regulated by synchrotron-generated microbeam radiotherapy (MRT) versus conventional broadbeam (CRT) in vitro using cultured EMT6.5 cells. We hypothesized (based on previous findings) that gene expression pathway changes after MRT are different from those seen CRT. found at 24 h postirradiation, exerts a broader regulatory effect multiple than involved transcription, translation initiation, macromolecule...

Synchrotron microbeam radiation treatment (MRT) is a preclinical radiotherapy technique with considerable clinical promise, although some of the underlying radiobiology MRT still not well understood. In recently reported studies, it has been suggested that elicits different tumor immune profile compared to broad-beam (BB). The aim this study was investigate effects synchrotron and BB on eosinophil-associated gene pathways eosinophil numbers within around in acute stage, 48 h postirradiation....

Cervical cancer is the most common and ranked as 4th in morbidity mortality among Malaysian women. Currently, Magnetic Resonance Imaging (MRI) considered gold standard imaging modality for tumours with a stage higher than IB2, due to its superiority diagnostic assessment of tumour infiltration excellent soft-tissue contrast. In this research, robustness semi-automatic segmentation has been evaluated using flood-fill algorithm quantitative feature extraction, 30 diffusion weighted MRI images...

Humanised xenograft models and cancer cell lines are widely used for preclinical drug evaluation, biological studies, targeted therapy strategies in research. A humanised mouse model is a laboratory that has been genetically modified to contain specific human genes, cells, or tissues. By introducing human-specific elements into rodents, researchers can create more accurate representation of physiological pathological processes. Lacking an appropriate animal osteosarcoma (OS), hindered...

Tamoxifen is an effective treatment for estrogen receptor alpha positive (ERa+)-breast cancer, however patients who received the five years have greater mortality risk compared to those did not receive tamoxifen. Furthermore, treated with tamoxifen developed resistance drug which mediated through p53 and PTEN. Therefore, study undertaken determine potential adjuvant properties of flavonoids, apigenin rutin promote anticancer activity induced by using ERa+-breast cancer MCF-7 cell lines....

Inhibition of telomerase activity has emerged as a promising strategy to combat cancer cells, especially ones with no specific molecular targets such triple negative breast (TNBC). Cisplatin, chemotherapeutic drug, is causing DNA damage while apigenin, plant-derived antioxidant, induces apoptosis in various cell types. Little known about their combined ability inhibit TNBC cells. In the current study, effect cisplatin combination apigenin was investigated regards and expression catalytic...

To evaluate the influence of iterative reconstruction (IR) levels on Computed Tomography (CT) image quality and to establish Figure Merit (FOM) value for CT Pulmonary Angiography (CTPA) examinations.Images 31 adult patients who underwent CTPA examinations in our institution from March April 2019 were retrospectively collected. Other data, such as scanning parameters, radiation dose body habitus information subjects also recorded. Six different IR applied volume data subjects. Five circles...

Cancer recurrence is often associated with the acquisition of radioresistance by cancer tissues due to failure in radiotherapy. The underlying mechanism leading development acquired EMT6 mouse mammary carcinoma cell line and potential pathway involved was investigated comparing differential gene expressions between parental cells. exposed 2 Gy/per cycle gamma-ray survival fraction EMT6-treated cells compared. EMT6RR_MJI (acquired radioresistance) developed after 8 cycles fractionated...

Abstract We presented a development of custom lead shield and mouse strainer for targeted irradiation from the gamma-cell chamber. This study was divided into two parts i.e., to (i) fabricate (Pb) (ii) optimize mice-bearing tumour model with 2 8 Gy absorbed doses. The shielding fabricated cuboid shape canal on top hole vertical side beam path. Respective deliveries doses 28 75 were used achieve at sites.

Cells apoptosis is characterized by chromatin condensation, cell shrinkage and intact membrane blebbing.The translocation of phosphotidylserine (PS) from the inner leaflets to outer layer serves as a critical event during apoptosis.Annexin V-FITC fluorescent probe which binds PS in Ca 2+ -dependent pathway.Once on surface, these are able bind annexin conjugate protein.Propium Iodide (PI) vital staining then performed demonstrate whether integrity has been preserved or become totally...

Abstract Radiotherapy plays an important role in cancer treatment; however, the incidence of radio-resistance remains become major challenges treatment. The presence epithelial-mesenchymal transition (EMT) has a clinical hallmark for to relapse after radiation aim this study was investigate morphological changes and cell survival EMT6 line post–gamma ray irradiation. irradiation cells conducted with two approaches, first, were treated single dose gamma-ray ranging low high (0-16 Gy) second,...

Introduction: The interaction between the T cell immune checkpoint proteins, programmed death-1 (PD-1) receptor, and its ligand PD-L1 plays a crucial role in suppression evasion of cancer cells from detection, thereby promoting tumour growth. Nivolumab, PD-1 inhibitor, disrupts this interaction, offering potential therapeutic anti-cancer strategy. goals study were to identify optimal dosage nivolumab that effectively decreases protein expression mouse model, examine impact on Methods: We...