A5068010653- Molecular Biology Techniques and Applications
- Forensic and Genetic Research
- Genetic diversity and population structure
- Environmental DNA in Biodiversity Studies
- Genetic Associations and Epidemiology
- Genomics and Phylogenetic Studies
- Genomics and Chromatin Dynamics
- Cancer-related molecular mechanisms research
- Pancreatic function and diabetes
- Erythrocyte Function and Pathophysiology
- melanin and skin pigmentation
- Cell Image Analysis Techniques
- Genetics, Bioinformatics, and Biomedical Research
- Glycosylation and Glycoproteins Research
- RNA regulation and disease
- Image Processing Techniques and Applications
Thermo Fisher Scientific (United States)
2015-2019
Thermo Fisher Scientific (Israel)
2014
Whitehead Institute for Biomedical Research
2006
Abstract Biologists can now prepare and image thousands of samples per day using automation, enabling chemical screens functional genomics (for example, RNA interference). Here we describe the first free, open-source system designed for flexible, high-throughput cell analysis, CellProfiler. CellProfiler address a variety biological questions quantitatively, including standard assays count, size, per-cell protein levels) complex morphological cell/organelle shape or subcellular patterns DNA staining).
SNPs that are molecularly very close (<10 kb) will generally have extremely low recombination rates, much less than 10−4. Multiple haplotypes often exist because of the history origins variants at different sites, rare recombinants, and vagaries random genetic drift and/or selection. Such multiallelic haplotype loci potentially important in forensic work for individual identification, defining ancestry, identifying familial relationships. The new DNA sequencing capabilities currently...
Today the primary DNA markers used in forensics are short tandem repeat (STR) polymorphisms (STRPs), initially selected because they highly polymorphic. However, increasingly common need to deal with samples a mixture of from two or more individuals sometimes is complicated by inherent stutter involved PCR amplification, especially strongly unbalanced mixtures when minor component coincides range major component. Also, STRPs use provide little evidence ancestry single source sample beyond...
Microhaplotypes have become a new type of forensic marker with great ability to identify and deconvolute mixtures because massively parallel sequencing (MPS) allows the alleles (haplotypes) multi-SNP loci be determined directly for an individual. As originally defined, microhaplotype locus is short segment DNA two or more SNPs defining three haplotypes. The length enough, less than about 300 bp, that read current MPS technology can produce phase-known sequence each chromosome part discovery...
Whole mitochondrial (mt) genome analysis enables a considerable increase in throughput, and improves the discriminatory power to maximum possible phylogenetic resolution. Most established protocols on different massively parallel sequencing (MPS) platforms, however, invariably involve PCR amplification of large fragments, typically several kilobases size, which may fail due mtDNA fragmentation available degraded materials. We introduce MPS tiling approach for simultaneous whole human mt...
Abstract Massively parallel sequencing is transforming forensic work by allowing various useful markers, such as STRPs and SNPs, to be multiplexed providing information on ancestry, individual familial identification, phenotypes for eye/hair/skin pigmentation, the deconvolution of mixtures. Microhaplotypes also become feasible with massively sequencing, these are DNA segments (smaller than 300 nucleotides) that selected contain multiple SNPs unambiguously defining three or more haplotype...
To perform a blind study to assess the capability of Ion Personal Genome Machine® (PGM™) system sequence forensically relevant genetic marker panels and characterize unknown individuals for ancestry possible relatedness.Twelve genomic samples were provided by third party blinded analysis. For these 12 samples, mitochondrial genome three PGM™ containing human identity single nucleotide polymorphisms (SNPs), informative SNPs, short tandem repeats (STRs) sequenced on analyzed.All four systems...