A5060731802- Influenza Virus Research Studies
- Respiratory viral infections research
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Viral gastroenteritis research and epidemiology
- Cardiac Imaging and Diagnostics
- T-cell and B-cell Immunology
- Ultrasound in Clinical Applications
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Diabetes and associated disorders
- Neuroscience of respiration and sleep
- SARS-CoV-2 detection and testing
- Pneumonia and Respiratory Infections
- Animal Virus Infections Studies
- Long-Term Effects of COVID-19
- vaccines and immunoinformatics approaches
- Monoclonal and Polyclonal Antibodies Research
Janssen (Netherlands)
2015-2024
Johnson & Johnson (Netherlands)
2015-2019
Leiden University Medical Center
2014
Safe and effective coronavirus disease–19 (COVID-19) vaccines are urgently needed to control the ongoing pandemic. While single-dose vaccine regimens would provide multiple advantages, two doses may improve magnitude durability of immunity protective efficacy. We assessed one- two-dose Ad26.COV2.S candidate in adult aged nonhuman primates (NHPs). A regimen induced higher peak binding neutralizing antibody responses compared with a single dose. In one-dose regimens, were stable for at least...
Human respiratory syncytial virus (RSV) poses a significant human health threat, particularly to infants and the elderly. While efficacious vaccines based on F protein have recently received market authorization, uncertainties remain regarding future need for vaccine updates counteract potential viral drift. The attachment G has long been ignored as target due perceived non-essentiality ineffective neutralization immortalized cells. Here, we show strong G-based in fully differentiated airway...
Respiratory syncytial virus (RSV) is a major cause of severe respiratory disease in infants and older people. Current RSV subunit vaccines are based on fusion protein that stabilized the prefusion conformation linked to heterologous foldon trimerization domain obtain F (preF) trimer. Here we show current induce undesirable anti-foldon antibodies non-human primates, mice humans. To overcome this, designed foldon-free preF trimer by elucidating structural basis trimerization-induced...
Synthetic long peptides (SLP) are a promising vaccine modality to induce therapeutic T cell responses in patients with chronic infections and tumors. We studied different formulations mice using SLP derived from carcinoembryonic Ag. discovered that one of the contains linear Ab epitope combination CD4 epitope. Repeated vaccination this Ag shows improved simultaneously induced high titers peptide-specific Abs. These Abs resulted unexpected anaphylaxis after third or subsequent vaccinations...
Various studies have shown that infants under the age of 6 months are especially vulnerable for complications due to influenza. Currently there no vaccines licensed use in this group. Vaccination pregnant women during last trimester, recommended by WHO as protective measure female population, may provide protection newborns at early age. Although it has been observed maternal vaccination can passively transfer protection, antibodies could possibly also interfere with subsequent active...
Abstract Safe and effective coronavirus disease (COVID)-19 vaccines are urgently needed to control the ongoing pandemic. While single-dose vaccine regimens would provide multiple advantages, two doses may improve magnitude durability of immunity protective efficacy. We assessed one- two-dose Ad26.COV2.S candidate in adult aged non-human primates (NHP). A regimen induced higher peak binding neutralizing antibody responses compared a single dose. In one-dose were stable for at least 14 weeks,...
Correlates of protection (CoP) are invaluable for iterative vaccine design studies, especially in pursuit complex vaccines such as a universal influenza (UFV) where single antigen is optimized to elicit broad against many viral antigenic variants. Since broadly protective antibodies virus often exhibit mutational evidence prolonged diversification, we studied germinal center (GC) kinetics hemagglutinin (HA) immunized mice. Here report that early 4 days after secondary immunization, the...
This staining procedure was established to assess the frequencies of various B- or T- cell subsets in single suspensions prepared from murine spleen lymph nodes. The protocol included describes required steps successfully stain samples with our developed and/or T-cell panel. B-cell panel allows identification plasmacells (CD19neg, CD138), Germinal center B-cells (CD19+ GL7+ CD95+) and Memory CD80+ PD-L2+). For detection antigen specific we have also a probe In current hemagglutinin as this...
This Competition ELISA was established to measure CR9114-like antibodies in murine serum. CR9114 is a monoclonal antibody which binds conserved stem region of the hemagglutinin (HA) protein influenza viruses. As binding site among broad variety viruses it associated with heterosubtypic protection. Identifying vaccines that elicit high titers will eventually help generation universal flu vaccine. protocol describes steps required successfully perform assay. A coating Rabbit IgG anti His-tag...
This ELISA was established to measure antibody titers against the haemagglutinin (HA) protein of influenza viruses in murine serum. protocol describes steps required successfully perform assay.