A5074359427- SARS-CoV-2 and COVID-19 Research
- Long-Term Effects of COVID-19
- COVID-19 Clinical Research Studies
- SARS-CoV-2 detection and testing
- Animal Virus Infections Studies
- Virus-based gene therapy research
- Viral gastroenteritis research and epidemiology
- Neuroinflammation and Neurodegeneration Mechanisms
- Animal Disease Management and Epidemiology
- Multiple Sclerosis Research Studies
- Bacillus and Francisella bacterial research
- Vector-Borne Animal Diseases
- Viral Infections and Vectors
- Mosquito-borne diseases and control
- Virology and Viral Diseases
- Immunotherapy and Immune Responses
- Respiratory viral infections research
- COVID-19 and Mental Health
- Bacteriophages and microbial interactions
- Influenza Virus Research Studies
- Molecular Biology Techniques and Applications
- Viral Infectious Diseases and Gene Expression in Insects
Institute for Veterinary Medical Research
2019-2025
Biomedical Primate Research Centre
2020-2024
Centre for Agricultural Research
2019
Hungarian Academy of Sciences
2019
The expanding pandemic of coronavirus disease 2019 (COVID-19) requires the development safe, efficacious and fast-acting vaccines. Several vaccine platforms are being leveraged for a rapid emergency response1. Here we describe candidate (YF-S0) severe acute respiratory syndrome 2 (SARS-CoV-2) that uses live-attenuated yellow fever 17D (YF17D) as vector to express noncleavable prefusion form SARS-CoV-2 spike antigen. We assess safety, immunogenicity efficacy in several animal models. YF-S0...
Safe and effective coronavirus disease–19 (COVID-19) vaccines are urgently needed to control the ongoing pandemic. While single-dose vaccine regimens would provide multiple advantages, two doses may improve magnitude durability of immunity protective efficacy. We assessed one- two-dose Ad26.COV2.S candidate in adult aged nonhuman primates (NHPs). A regimen induced higher peak binding neutralizing antibody responses compared with a single dose. In one-dose regimens, were stable for at least...
SARS-CoV-2 causes acute respiratory disease, but many patients also experience neurological complications. Neuropathological changes with pronounced neuroinflammation have been described in individuals after lethal COVID-19, as well the CSF of hospitalized To assess whether neuropathological can occur a infection, leading to mild-to-moderate we investigated brains four rhesus and cynomolgus macaques pulmonary disease without overt clinical symptoms. Postmortem analysis demonstrated...
Coronavirus disease 2019 (COVID-19) patients initially develop respiratory symptoms, but they may also suffer from neurological symptoms. People with long-lasting effects after acute infections severe syndrome coronavirus 2 (SARS-CoV-2), i.e., post-COVID or long COVID, experience a variety of manifestations. Although we do not fully understand how SARS-CoV-2 affects the brain, neuroinflammation likely plays role.
The post-acute phase of SARS-CoV-2 infection was investigated in rhesus (Macaca mulatta) and cynomolgus macaques fascicularis). During the acute infection, shed via nose throat, viral RNA occasionally detected feces. This coincided with a transient change systemic immune activation. Even after alleged resolution computed tomography (CT) positron emission (PET)-CT revealed pulmonary lesions activated tracheobronchial lymph nodes all animals. Post-mortem histological examination lung tissue...
Assays to measure SARS-CoV-2-specific neutralizing antibodies are important monitor seroprevalence, study asymptomatic infections and reveal (intermediate) hosts. A recently developed assay, the surrogate virus-neutralization test (sVNT) is a quick commercially available alternative "gold standard" virus neutralization assay using authentic virus, does not require processing at BSL-3 level. The relies on inhibition of binding receptor domain (RBD) spike (S) protein human...
Abstract SARS-CoV-2 may cause acute respiratory disease, but the infection can also initiate neurological symptoms. Here we show that causes brain inflammation in macaque model. An increased metabolic activity pituitary gland of two macaques was observed by longitudinal positron emission tomography-computed tomography (PET-CT). Post-mortem analysis demonstrated infiltration T-cells and activated microglia brain, viral RNA detected tissues from one animal. We Lewy bodies brains all rhesus...
Novel safe, immunogenic, and effective vaccines are needed to control the COVID-19 pandemic, caused by SARS-CoV-2. Here, we describe safety, robust immunogenicity, potent efficacy elicited in rhesus macaques a modified vaccinia virus Ankara (MVA) vector expressing full-length SARS-CoV-2 spike (S) protein (MVA-S). MVA-S vaccination was well tolerated induced S receptor-binding domain (RBD)-binding IgG antibodies neutralizing against several variants of concern. S-specific IFNγ, but not IL-4,...
Abstract Among the common strategies to design next-generation COVID-19 vaccines is broadening antigenic repertoire thereby aiming increase efficacy against emerging variants of concern (VoC). This study describes a new Orf virus-based vector (ORFV) platform multiantigenic vaccine targeting SARS-CoV-2 spike and nucleocapsid antigens. Vaccine candidates were engineered, either expressing protein (ORFV-S) alone or co-expressing (ORFV-S/N). Mono- elicited comparable levels spike-specific...
Abstract The next generation of COVID-19 vaccines needs to broaden the antigenic repertoire improve breadth immune response and efficacy against emerging variants concern. This study describes a new parapoxvirus-based vector (ORFV) as platform design multi-antigenic vaccine targeting SARS-CoV-2 spike nucleocapsid antigens. Two candidates were engineered, one expressing protein alone (ORFV-S) other co-expressing more conserved (ORFV-S/N). Both elicited comparable levels spike-specific...
The aim of this study was to investigate the application [18F]DPA714 visualize inflammation process in lungs SARS-CoV-2-infected rhesus monkeys, focusing on presence pulmonary lesions, activation mediastinal lymph nodes and surrounded lung tissue.Four experimentally SARS-CoV-2 infected monkeys were followed for seven weeks post infection (pi) with a weekly PET-CT using [18F]DPA714. Two PET images, 10 min each, single field-of-view covering chest area, obtained 30 after injection. To...
Abstract Influenza virosomes serve as antigen delivery vehicles and pre-existing immunity toward influenza improves the immune responses antigens. Here, vaccine efficacy was evaluated in non-human primates with a COVID-19 virosome-based containing low dose of RBD protein (15 µg) adjuvant 3M-052 (1 µg), displayed together on virosomes. Vaccinated animals (n = 6) received two intramuscular administrations at week 0 4 challenged SARS-CoV-2 8, unvaccinated control 4). The safe well tolerated...
Abstract Safe and effective coronavirus disease (COVID)-19 vaccines are urgently needed to control the ongoing pandemic. While single-dose vaccine regimens would provide multiple advantages, two doses may improve magnitude durability of immunity protective efficacy. We assessed one- two-dose Ad26.COV2.S candidate in adult aged non-human primates (NHP). A regimen induced higher peak binding neutralizing antibody responses compared a single dose. In one-dose were stable for at least 14 weeks,...
The presence of a novel adenovirus (AdV) was detected by PCR and sequencing, in the internal organs captive polar bear that had died Budapest zoo. virus content samples proved to be high enough allow for conventional Sanger sequencing on PCR-amplified genomic fragments. With this approach, sequence entire genome putative 1 (PBAdV-1) obtained. Although found short, consisting 27,952 base pairs merely, with relatively balanced G + C 46.3 %, its organisation corresponded largely typical...
Abstract The post-acute phase of SARS-CoV-2 infection was investigated in rhesus macaques ( Macaca mulatta ) and cynomolgus fascicularis ). During the acute infection, shed via nose throat, viral RNA occasionally detected feces. This coincided with a transient change systemic immune activation. Even after alleged resolution as suggested by absence nasal tracheal swabs, computed tomography (CT) positron emission (PET)-CT were able to reveal pulmonary lesions activated tracheobronchial lymph...
Abstract Infection with severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) impacts multiple anatomical sites. Whether this is due to the virus itself or a secondary effect caused by influx and activation of immune cells not known. Positron emission tomography (PET) immunoglobulins can provide insights into which sites are activated in living animal. Our aim use two nanobodies as tools monitor (1) distribution antigen presenting (APC) virtue their Mafa‐DR expression profile, (2)...
Background: Recently, an emerging flavivirus, Usutu virus (USUV), has caused epidemic among birds in Europe, resulting a massive die-off Eurasian blackbirds. Currently found only Europe and Africa, it can be envisioned that will follow the path of other flaviviruses, like West Nile Zika virus, spread via its mosquito vectors bird hosts to parts world. Several cases human infections by have already been published. Anticipating this spread, development efficacious vaccine would highly...
Infection of an adult rhesus macaque with SARS-CoV-2 led to viral RNAemia in nose, throat, and lungs. The animal also presented extended fecal shedding genomic subgenomic messenger RNA replication-competent virus for more than 3 weeks after infection. Positron emission tomography revealed increased intestinal glucose metabolism which was histologically related inflammation the ileum. These findings highlight potential cause gastrointestinal infections macaques like this is regularly observed...
Bovine adenoviruses (BAdV) are known to cause respiratory and/or intestinal disease in calves. Infection can manifest as acute outbreaks, but more often only sporadic cases occur. Here we describe the PCR detection and partial sequence characterization of several BAdVs found sick or dead calves on different farms Western Hungary. Intermittent diarrhoeal illnesses occurred after weaning among located up 40 km apart. A high-sensitivity, broad-spectrum nested PCR, developed for general...
Abstract Assays to measure SARS-CoV-2-specific neutralizing antibodies are important monitor seroprevalence, study asymptomatic infections and reveal (intermediate) hosts. A recently developed assay, the surrogate virus-neutralization test (sVNT) is a quick commercially available alternative “gold standard” virus neutralization assay using authentic virus, does not require processing at BSL-3 level. The relies on inhibition of binding receptor domain (RBD) spike (S) protein human...