A5071884062- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Viral Infections and Outbreaks Research
- SARS-CoV-2 and COVID-19 Research
- Influenza Virus Research Studies
- RNA and protein synthesis mechanisms
- Insect symbiosis and bacterial influences
- interferon and immune responses
- Immune Response and Inflammation
- Protein Structure and Dynamics
- Bacillus and Francisella bacterial research
- Viral gastroenteritis research and epidemiology
- Hospital Admissions and Outcomes
- HIV Research and Treatment
- Insect Utilization and Effects
- Virology and Viral Diseases
- Vibrio bacteria research studies
- COVID-19 Clinical Research Studies
- Vector-borne infectious diseases
- Emergency and Acute Care Studies
Rega Institute for Medical Research
2020-2024
KU Leuven
2020-2024
Global Virus Network
2020-2022
Chang Gung University
2016-2018
The expanding pandemic of coronavirus disease 2019 (COVID-19) requires the development safe, efficacious and fast-acting vaccines. Several vaccine platforms are being leveraged for a rapid emergency response1. Here we describe candidate (YF-S0) severe acute respiratory syndrome 2 (SARS-CoV-2) that uses live-attenuated yellow fever 17D (YF17D) as vector to express noncleavable prefusion form SARS-CoV-2 spike antigen. We assess safety, immunogenicity efficacy in several animal models. YF-S0...
ABSTRACT The NS1 protein encoded by influenza A virus antagonizes the interferon response through various mechanisms, including blocking cellular mRNA maturation binding CPSF30 3′ end processing factor and/or suppressing activation of regulatory 3 (IRF3). In present study, we identified two truncated proteins that are translated from internal AUGs at positions 235 and 241 open reading frame. We analyzed localization function N-truncated strains, Udorn/72/H3N2 (Ud) Puerto Rico/8/34/H1N1...
Influenza A virus, which can cause severe respiratory illnesses in infected individuals, is responsible for worldwide human pandemics. The NS1 protein encoded by this virus plays a crucial role regulating the host antiviral response through various mechanisms. In addition, it has been reported that modulate cellular pre-mRNA splicing events. To investigate biological processes potentially affected cells, NS1-associated complexes cells were identified using coimmunoprecipitation combined with...
Influenza A virus infections can result in severe respiratory diseases. The H7N9 subtype of avian influenza has been transmitted to humans and caused disease death. Nonstructural protein 1 (NS1) is a virulence determinant during viral infection. To elucidate the functions NS1 encoded by (H7N9 NS1), interaction partners human cells were identified with immunoprecipitation followed SDS-PAGE coupled liquid chromatography-tandem mass spectrometry (GeLC–MS/MS). We 36 cellular proteins as...
Abstract The explosively expanding COVID-19 pandemic urges the development of safe, efficacious and fast-acting vaccines to quench unrestrained spread SARS-CoV-2. Several promising vaccine platforms, developed in recent years, are leveraged for a rapid emergency response 1 . We employed live-attenuated yellow fever 17D (YF17D) as vector express prefusion form SARS-CoV-2 Spike antigen. In mice, candidate, tentatively named YF-S0, induces high levels neutralizing antibodies favorable Th1...
To curb viral epidemics and pandemics, antiviral drugs are needed with activity against entire genera or families of viruses. Here, we develop a cell-based multiplex assay for high-throughput screening multiple viruses at once, as demonstrated by using three distantly related orthoflaviviruses: dengue, Japanese encephalitis yellow fever virus. Each virus is tagged distinct fluorescent protein, enabling individual monitoring in cell culture through high-content imaging. Specific antisera...
Fast and accurate detection of neutralizing antibodies (nAbs) against yellow fever virus (YFV) is key in serodiagnosis, outbreak surveillance, monitoring vaccine efficacy. Although classical PRNT remains the gold standard for measuring YFV nAbs, this methodology suffers from inherent limitations such as low throughput overall high labor intensity.
New platforms are needed for the design of novel prophylactic vaccines and advanced immune therapies. Live-attenuated yellow fever vaccine YF17D serves as a vector several licensed platform candidates. On basis YF17D, we developed an exceptionally potent COVID-19 candidate called YF-S0. However, use such live RNA viruses raises safety concerns, adverse events linked to original (yellow vaccine-associated neurotropic disease [YEL-AND] viscerotropic [YEL-AVD]). In this study, investigated...
ABSTRACT Aedes aegypti mosquitoes can transmit several arboviruses, including chikungunya virus (CHIKV), dengue (DENV), and Zika (ZIKV). When blood-feeding on a virus-infected human, the mosquito ingests into midgut (stomach), where it replicates must overcome barrier to disseminate other organs ultimately be transmitted via saliva. Current tools study mosquito-borne viruses (MBVs) include 2D-cell culture systems in vivo infection models, which offer great advantages, yet have some...
ABSTRACT New platforms are urgently needed for the design of novel prophylactic vaccines and advanced immune therapies. Live-attenuated yellow fever vaccine YF17D serves as vector several licensed platform candidates. Based on YF17D, we developed YF-S0 exceptionally potent COVID-19 candidate. However, use such live RNA virus raises safety concerns, i . e ., adverse events linked to original (yellow vaccine-associated neurotropic; YEL-AND, viscerotropic disease; YEL-AVD). In this study,...
Abstract Aedes aegypti mosquitoes can transmit several arboviruses, including chikungunya virus (CHIKV), dengue (DENV), and Zika (ZIKV). When blood-feeding on a virus-infected human, the mosquito ingests into midgut (stomach), where it replicates must overcome barrier to disseminate other organs ultimately be transmitted via saliva. Current tools study mosquito-borne viruses (MBVs) include 2D-cell culture systems in vivo infection models, which offer great advantages, yet have some...
Abstract Quick and accurate detection of neutralizing antibodies (nAbs) against yellow fever is essential in serodiagnosis during outbreaks, for surveillance to evaluate vaccine efficacy population-wide studies. All this requires serological assays that can process a large number samples highly standardized format. Albeit being laborious, time-consuming limited throughput, classical plaque reduction neutralization test (PRNT) still considered gold standard the quantification nAbs due its...