A5061602962- RNA modifications and cancer
- Salivary Gland Disorders and Functions
- Peptidase Inhibition and Analysis
- Advanced Proteomics Techniques and Applications
- Tryptophan and brain disorders
- Attention Deficit Hyperactivity Disorder
- RNA Research and Splicing
- interferon and immune responses
- Head and Neck Cancer Studies
- Salivary Gland Tumors Diagnosis and Treatment
- Monoclonal and Polyclonal Antibodies Research
- Bladder and Urothelial Cancer Treatments
- Hepatocellular Carcinoma Treatment and Prognosis
- Heme Oxygenase-1 and Carbon Monoxide
- Cancer-related molecular mechanisms research
- RNA and protein synthesis mechanisms
- Oral Health Pathology and Treatment
- Ultrasound and Hyperthermia Applications
- Inflammasome and immune disorders
- Viral-associated cancers and disorders
- Veterinary Oncology Research
- Metabolomics and Mass Spectrometry Studies
- Photoacoustic and Ultrasonic Imaging
- Histiocytic Disorders and Treatments
- Hormonal Regulation and Hypertension
Chang Gung University
2015-2024
Chang Gung Memorial Hospital
2011-2024
Linkou Chang Gung Memorial Hospital
2014-2024
Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital
2024
Marshfield Clinic
2024
Marshfield Clinic
2024
National Taiwan University Hospital
2020-2024
Saint Louis University
2024
Molecular Medicine Research Institute
2010-2023
University of Minnesota Medical Center
2022-2023
Transcatheter arterial chemoembolization (TACE) improves the treatment of hepatocellular carcinoma (HCC) by causing tumour necrosis and shrinkage. Fifty-two patients with resectable large HCC (defined as a maximal diameter 10 cm or more) were prospectively randomized into two groups: group 1 comprised 24 who had 1-5 sessions TACE before operation; 2 consisted other 28 patients, on whom surgery was performed without delay. Tumour volume reduced to mean (s.d.) 42.8 (15.3) per cent in 16 1, but...
Abstract Photodynamic therapy (PDT) is a kind of photochemo‐therapeutic treatment that exerts its effect mainly through the induction cell death. Distinct types death may be elicited by different PDT regimes. In this study, mechanisms involved in human epidermoid carcinoma A431 cells triggered with Photofrin® (a clinically approved photosensitizer) were characterized. Photofrin distributes dynamically cells; plasma membranes and Golgi complex are main target sites after brief (3 h) prolonged...
Bladder cancer is a common urologic whose incidence continues to rise annually. Urinary microparticles are an attractive material for noninvasive bladder biomarker discovery. In this study, we applied isotopic dimethylation labeling coupled with liquid chromatography-tandem mass spectrometry (LC–MS/MS) discover biomarkers in urinary isolated from hernia (control) and patients. This approach identified 2964 proteins based on more than two distinct peptides, of which 2058 had not previously...
Although cancer cell secretome profiling is a promising strategy used to identify potential body fluid-accessible biomarkers, questions remain regarding the depth which can be mined and efficiency with researchers select useful candidates from growing list of identified proteins. Therefore, we analyzed secretomes 23 human lines derived 11 types using one-dimensional SDS-PAGE nano-LC-MS/MS performed on an LTQ-Orbitrap mass spectrometer generate more comprehensive secretome. A total 31,180...
A urine sample preparation workflow for the iTRAQ (isobaric tag relative and absolute quantitation) technique was established. The reproducibility of this platform evaluated applied to discover proteins with differential levels between pooled samples from nontumor controls three bladder cancer patient subgroups different grades/stages (a total 14 23 cases in two multiplex runs). Combining results independent clinical sets, a 638 were identified. Among them, 55 consistently showed >2-fold...
Abstract The cancer cell secretome may contain potentially useful biomarkers. In this study, we integrated the profiles of secreted proteins in lung lines with mRNA expression levels from pulmonary adenocarcinoma tissue, a view to identify effective biomarkers for non‐small (NSCLC). Among novel candidates isolated, importin subunit alpha‐2 (also known as karyopherin alpha [KPNA]‐2), was selected further validation. Immunohistochemical staining revealed overexpression KPNA2 nuclei tumor...
In this study, we evaluated the reproducibility of abundant urine protein depletion by hexapeptide-based library beads and an antibody-based affinity column using iTRAQ technique. The affinity-depletion approach, which proved superior, was then applied in conjunction with to discover proteins that were differentially expressed between pooled samples from hernia bladder cancer patients. Several proteins, including seven apolipoproteins, TIM, SAA4, proEGF further verified 111 203 individual...
Significance Oral squamous cell carcinoma (OSCC) accounts for 90% of oral cancers, and earlier detection efficiently increases the survival rate. Here, we used a comprehensive literature review to select candidates LC-multiple reaction monitoring-MS qualitatively quantitatively measure target proteins in saliva samples from individuals at-risk population Taiwan. Statistical analysis results establishes four-protein panel sufficient detect 88.6% early-stage patients 91.6% all with 80.4%...
Oral squamous cell carcinoma is a prominent cancer worldwide, particularly in Taiwan. By integrating omics analyses 50 matched samples, we uncover Taiwanese patients predominant mutation signature associated with cytidine deaminase APOBEC, which correlates the upregulation of APOBEC3A expression APOBEC3 gene cluster at 22q13. significantly higher tumors carrying APOBEC3B-deletion allele(s). High-level better overall survival, especially among alleles, as examined second cohort (n = 188; p...
Oral cavity squamous cell carcinoma (OSCC), which is frequently associated with poor prognosis and mortality, a leading cause of cancer-related death worldwide. Discovery body fluid accessible biomarkers needed to improve OSCC screening. To this end, we profiled proteomes saliva from the healthy volunteers, individuals oral potentially malignant disorders (OPMD), patients by means SDS-PAGE coupled LC-MS/MS. In control, OPMD, groups, 958, 845, 1030 salivary proteins were detected,...
Oral cavity squamous cell carcinoma (OSCC) is one of the most common cancers worldwide. In Taiwan, OSCC fifth leading cause cancer-related mortality and leads to 2800 deaths per year. The poor outcome patients principally ascribed fact that this disease often advanced at time diagnosis, suggesting early detection urgently needed. Analysis body fluids promising approach identify biomarker candidates cancers. To biomarkers, salivary proteomes patients, individuals with oral potentially...
Abstract Ultraviolet (UV) light is a strong apoptotic trigger that induces caspase‐dependent biochemical changes in cells. Previously we showed UV irradiation can activate caspase‐3, and the subsequent cleavage activation of p21 Cdc42/Rac ‐activated kinase 2 (PAK2) human epidermoid carcinoma A431 In this study demonstrate curcumin (Cur), yellow pigment Curcuma longa with known anti‐oxidant anti‐inflammatory properties, prevent irradiation‐induced changes, including c‐Jun N‐terminal (JNK)...
Abstract The cancer cell secretome may contain many potentially useful biomarkers. We therefore sought to identify proteins in the conditioned media of colorectal carcinoma (CRC) lines but not those from other lines. secretomes 21 derived 12 types were analyzed by SDS‐PAGE combined with MALDI‐TOF MS. Among 325 identified, collapsin response mediator protein‐2 (CRMP‐2) was chosen for evaluation as a potential CRC biomarker, since it selectively detected line and has never been reported...
Lung cancer is the leading cause of cancer-related death worldwide. Both diagnostic and prognostic biomarkers are urgently needed to increase patient survival. In this study, we identified/quantified 1763 proteins from paired adenocarcinoma (ADC) tissues with different extents lymph node (LN) involvement using an iTRAQ-based quantitative proteomic analysis. Based on a bioinformatics analysis literature search, selected six candidates (ERO1L, PABPC4, RCC1, RPS25, NARS, TARS) set 133 that...
Glucose-6-phosphate dehydrogenase (G6PD) is the rate-limiting enzyme of pentose phosphate pathway that modulates cellular redox homeostasis via regeneration NADPH. G6PD-deficient cells have a reduced ability to induce innate immune response, thus increasing host susceptibility pathogen infections. An important part response activation inflammasome. peripheral blood mononuclear (PBMCs) from patients and human monocytic (THP-1) were used as models investigate whether G6PD inflammasome...
Abstract In the long history of traditional Chinese medicine, single herbs and complex formulas have been suggested to increase lifespan. However, identification molecules responsible for lifespan extension has challenging. Here, we collected a list medicines with potential longevity properties from pharmacopeias. By utilizing mother enrichment program, systematically screened these identified herb, Psoralea corylifolia , that increases in Saccharomyces cerevisiae. Next, twenty-two pure...
Although liver resection is now a safe procedure, its role for hepatocellular carcinoma (HCC) in patients with cirrhosis remains controversial.This study compared the results of HCC over two time intervals. One hundred and sixty-one had during period 1 (1991-1996) 265 2 (1997-2002). Early long-term after periods were compared, clinicopathological characteristics that influenced survival identified.Tumour size was smaller, indocyanine green retention rate higher, older greater proportion...
Nasopharyngeal carcinoma (NPC) is commonly diagnosed late due to its deep location and vague symptoms. To identify biomarkers for early NPC diagnosis, secreted proteomes of two cell lines were analyzed. Proteins in the cell-line cultured media systematically identified by SDS-PAGE combined with MALDI-TOF MS. Twenty-three proteins found from both lines. Among them, fibronectin, Mac-2 binding protein (Mac-2 BP), plasminogen activator inhibitor 1 (PAI-1) further confirmed Western blot analysis....
Intermittent clamping of the hepatic pedicle during hepatectomy may reduce operative bleeding, but its limitations and long-term effects on cirrhotic liver are unknown. Eighty-three patients with cirrhosis undergoing repeated for 15 min declamping 5 were divided into three groups based total duration: group 1 less than 40 (39 patients); 2 40-80 (28); 3 more 80 (16). Larger tumours associated longer ischaemia times (P = 0.002), operating times, greater blood loss increased transfusion...
Abstract Background Liver resection is the main curative treatment for hepatocellular carcinoma (HCC), but recurrence rates are high. The remnant liver most common site of recurrence, role repeat hepatectomy in recurrent HCC controversial. Methods Patients who underwent and subsequent between 1990 2007 were reviewed retrospectively. Clinicopathological characteristics, early- long-term outcomes patients had a first, second, third fourth compared. Results Some 1177 first HCC, 149, 35 eight...
Nasopharyngeal carcinoma (NPC) is one of the most common malignant tumors in Southeast Asia. Unfortunately, NPC victims have had metastasis when first diagnosed due to its deep location and vague symptoms. To date, discovery sensitive specific biomarkers for improving detection remains a challenge. Our previous study established strategy cell secretome analysis using hollow fiber culture (HFC) system combined with liquid chromatography mass spectrometry. Herein, above platform was used...
Oral cavity squamous cell carcinoma (OSCC) is a devastating disease that accounts for 3% of all cancer cases diagnosed annually. OSCC usually at advanced clinical stages, resulting in poor outcomes. To identify effective biomarkers improved diagnosis and/or management, we simultaneously analyzed the secretome and tissue transcriptome. Among 19 candidates isolated, guanylate-binding protein 1 (GBP1) was selected further validation using serum samples from patients healthy controls. Notably,...