A5103121345- Antibiotic Resistance in Bacteria
- Pharmaceutical and Antibiotic Environmental Impacts
- Antibiotics Pharmacokinetics and Efficacy
- Pneumonia and Respiratory Infections
- RNA modifications and cancer
- Retinoids in leukemia and cellular processes
- RNA regulation and disease
- Bacteriophages and microbial interactions
- Cancer Genomics and Diagnostics
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Acute Myeloid Leukemia Research
- Enterobacteriaceae and Cronobacter Research
- Sepsis Diagnosis and Treatment
- Hemodynamic Monitoring and Therapy
- Endoplasmic Reticulum Stress and Disease
- Mechanisms of cancer metastasis
- Mitochondrial Function and Pathology
- Genomics and Phylogenetic Studies
- Escherichia coli research studies
- Nuclear Structure and Function
- Vibrio bacteria research studies
- Immune Cell Function and Interaction
- Microbial infections and disease research
- Lung Cancer Treatments and Mutations
Chang Gung University
2016-2025
Sir Run Run Shaw Hospital
2018-2025
Zhejiang University
2013-2025
Linkou Chang Gung Memorial Hospital
2021-2025
Chang Gung Memorial Hospital
2019-2023
Chang Gung Children's Hospital
2022-2023
Molecular Medicine Research Institute
2022
China Medical University
2021
First Affiliated Hospital of Gannan Medical University
2019
First Affiliated Hospital Zhejiang University
2014-2015
Significance Oral squamous cell carcinoma (OSCC) accounts for 90% of oral cancers, and earlier detection efficiently increases the survival rate. Here, we used a comprehensive literature review to select candidates LC-multiple reaction monitoring-MS qualitatively quantitatively measure target proteins in saliva samples from individuals at-risk population Taiwan. Statistical analysis results establishes four-protein panel sufficient detect 88.6% early-stage patients 91.6% all with 80.4%...
Oral squamous cell carcinoma is a prominent cancer worldwide, particularly in Taiwan. By integrating omics analyses 50 matched samples, we uncover Taiwanese patients predominant mutation signature associated with cytidine deaminase APOBEC, which correlates the upregulation of APOBEC3A expression APOBEC3 gene cluster at 22q13. significantly higher tumors carrying APOBEC3B-deletion allele(s). High-level better overall survival, especially among alleles, as examined second cohort (n = 188; p...
Enterobacter spp. are members of the 'ESKAPE' group pathogens, which recognised as leading cause multidrug-resistant (MDR) hospital-acquired infections. Colistin is usually regarded a last-line therapeutic option for MDR Gram-negative bacilli However, colistin-resistant have emerged in last decade. Here we investigated prevalence colistin resistance and mcr genes clinical origin between 2011 2020 tertiary hospital China. rates ranged 17.1% 34.5%, with an overall 22.2% (190/854). No mcr-1 to...
Adenosine-to-inosine RNA editing constitutes a crucial component of the cellular transcriptome and critically underpins organism survival development. While recent high-throughput approaches have provided comprehensive documentation editome, its functional output remains mostly unresolved, particularly for events in non-coding regions. Gene ontology analysis known targets unveiled preponderance genes related to apoptosis regulation, among which proto-oncogenes XIAP MDM2 encode two most...
ObjectivesCommunity onset urinary tract infections (COUTIs) drew attention recently owing to their increased prevalence and associations with resistant pathogens. The study is aimed at investigating the etiology of COUTIs as well related risk factors extended-spectrum β-lactamase (ESBL) in China.MethodsThe prospective was performed nineteen hospitals during November 1, 2017 August 31, 2019. Non-duplicated isolates from were included. ESBL phenotypic confirmation test whole genomes sequenced...
Vibrio parahaemolyticus is a common food-borne pathogen that normally associated with seafood. In 1996, pandemic O3:K6 strain abruptly appeared and caused the first of this to spread throughout many Asian countries, America, Europe, Africa. The role temperate bacteriophages in evolution great interest. work, new phage, VP882, from V. was purified characterized after mitomycin C induction. VP882 Myoviridae bacteriophage polyhedral head long rigid tail sheath-like structure. It infected lysed...
Along with the constant improvement in high-throughput sequencing technology, an increasing number of transcriptome projects are carried out organisms without decoded genome information and even on environmental biological samples. To study functions novel transcripts, very first task is to identify their potential functions. We present a web-based annotation tool, FunctionAnnotator, which offers comprehensive annotations, including GO term assignment, enzyme annotation, domain/motif...
Mycoplasma pneumoniae is currently the most commonly detected bacterial cause of childhood community-acquired pneumonia in several countries. Of note, clonal expansion macrolide-resistant ST3 occurred Japan and South Korea. An alarming surge macrolide resistance complicates treatment pneumonia. We aimed to evaluate clinical manifestation relatedness M. circulating among children Taiwan.We prospectively enrolled 626 with radiologically confirmed between 2017 2019. infection was suspected on...
Oral cavity squamous cell carcinoma (OSCC), a leading subtype of head and neck cancer, has high global incidence mortality rates. Despite surgery radiochemotherapy, about one-third patients relapse. Targeted immunotherapy options for recurrent OSCC show limited effectiveness, underscoring the need improved treatments. We generated multi-omics datasets from pretreatment samples (n=137) to identify A3A EGFR as markers precise patient stratification. Survival outcomes were assessed by comparing...
Abstract Background Omicron poses significant concerns, leading to two severe complications, encephalitis and croup in children. Neutrophil predominance lymphopenia were observed cases. We propose a distinctive blood RNA signature characterize COVID-19's immune landscape, potentially aiding differentiating from mild cases pediatric patients, with implications for clinical management. Volcano plot of differentially expressed genes between disease Fold change was the ratio average expression...
Hemodynamic management is of paramount importance in patients with septic shock. Echocardiography has been increasingly used assessing volume status and cardiac function. However, whether the utilization echocardiography an impact on prognosis unknown. Thus, we intended to explore its effect outcomes shock.The study was based Medical Information Mart for Intensive Care (MIMIC) III database. Septic shock were divided into two groups according usage during onset The primary outcome 28-day...
Background: Probiotics have been previously reported to reduce the incidence of necrotizing enterocolitis (NEC) in extremely preterm infants, but mechanisms by which probiotics work remain unknown. We aimed investigate effects on gut microbiota infants. Methods: A prospective cohort study was conducted 120 neonates (gestational age ≤ 28 weeks) between August 2019 and December 2021. All were divided into (receiving probiotics) control (no groups. Multivariate logistic regression analysis...
ABSTRACT We report the genome organization and analysis of first completely sequenced T4-like phage, AR1, Escherichia coli O157:H7. Unlike most other phages O157:H7, which belong to temperate Podoviridae Siphoviridae families, AR1 is a phage known efficiently infect this pathogenic bacterial strain. The 167,435-bp currently largest among all E. O157:H7 phages. It carries total 281 potential open reading frames (ORFs) 10 putative tRNA genes. Of these, 126 predicted proteins could be...
Abstract Because of innumerable cancer sequencing projects, abundant transcriptome expression profiles together with survival data are available from the same patients. Although some signatures for prognosis or pathologic staging have been identified these data, systematically discovering such kind remains a challenge. To address this, we developed TACCO (Transcriptome Alterations in CanCer Omnibus), database identifying differentially expressed genes and altered pathways cancer. also...
Dissemination of multidrug-resistant, particularly tigecycline-resistant, Acinetobacter baumannii is critical importance, as tigecycline considered a last-line antibiotic. Acquisition tet(X), tigecycline-inactivating enzyme mostly found in strains animal origin, imparts resistance to A. baumannii. Herein, we investigated the presence tet(X) variants among 228 tigecycline-non-susceptible isolates from patients at Taiwanese hospital via polymerase chain reaction using newly designed universal...
Golgin-97 is a tethering factor in the trans-Golgi network (TGN) and crucial for vesicular trafficking maintaining cell polarity. However, significance of golgin-97 human diseases such as cancer remains unclear. We searched potential role cancers using Kaplan-Meier Plotter ( http://kmplot.com ) Oncomine www.oncomine.org datasets. Specific functions migration invasion were examined golgin-97-knockdown golgin-97-overexpressing cells. cDNA microarray, pathway analysis qPCR used to identify gene...
The coinfection process producing multiple species of pathogens provides a specific ecological niche for the exchange genetic materials between pathogens, in which plasmids play vital role horizontal gene transfer, especially drug resistance, but underlying transfer pathway remains unclear. Interspecies communication associated with third-generation cephalosporins, quinolones, and colistin resistance has been observed simultaneously isolated Escherichia coli Klebsiella pneumoniae from...
Abstract Incidence of invasive pneumococcal disease caused by antimicrobial-resistant Streptococcus pneumoniae types not included in conjugate vaccines has increased, including a penicillin- and meropenem-resistant serotype 15A-ST63 clone Japan. During 2013–2017, we collected 206 isolates Taiwan for penicillin meropenem susceptibility testing. We found serotypes 15B/C-ST83 were the most prevalent clones. A transformation study confirmed that penicillin-binding protein (PBP) 2b was primary...
We previously identified matrix metalloproteinase-1 (MMP-1) as one of the most promising salivary biomarkers for oral squamous cell carcinoma (OSCC) and developed a sensitive ELISA MMP-1 with good performance in detection OSCC using cohort 1160 saliva samples.
Background: Early identification of critically ill neonates with poor outcomes can optimize therapeutic strategies. We aimed to examine whether machine learning (ML) methods improve mortality prediction for neonatal intensive care unit (NICU) patients on intubation respiratory failure. Methods: A total 1734 failure were randomly divided into training (70%, n = 1214) and test (30%, 520) sets. The primary outcome was the probability NICU mortality. areas under receiver operating characteristic...
Genomic changes in Mycoplasma pneumoniae caused by adaptation to environmental or ecologic pressures are poorly understood. We collected M. from children who had confirmed pneumonia Taiwan during 2017-2020. used whole-genome sequencing compare these isolates with a worldwide collection of current and historical clinical strains for characterizing population structures. A phylogenetic tree 284 showed that all sequenced consisted 5 clades: T1-1 (sequence type [ST]1), T1-2 (mainly ST3), T1-3...
Whole genome sequence construction is becoming increasingly feasible because of advances in next generation sequencing (NGS), including increasing throughput and read length. By simply overlapping paired-end reads, we can obtain longer reads with higher accuracy, which facilitate the assembly process. However, influences different library sizes methods on sequencing-based de novo remain poorly understood. We used 250 bp Illumina Miseq generated from genomic DNA Escherichia coli DH1...
Lung adenocarcinoma (ADC) is the predominant histological type of lung cancer, and radiotherapy one current therapeutic strategies for cancer treatment. Unfortunately, biological complexity heterogeneity contribute to radioresistance development. Karyopherin α2 (KPNA2) a member importin α family that mediates nucleocytoplasmic transport cargo proteins. KPNA2 overexpression observed across tissues diverse origins. However, role in unclear. Herein, we demonstrated high expression positively...