A5022543698- RNA modifications and cancer
- Cancer Genomics and Diagnostics
- Glioma Diagnosis and Treatment
- Cancer-related molecular mechanisms research
- Colorectal Cancer Treatments and Studies
- RNA Research and Splicing
- MicroRNA in disease regulation
- Retinoids in leukemia and cellular processes
- Lung Cancer Treatments and Mutations
- Genomics and Phylogenetic Studies
- Circular RNAs in diseases
- Pneumonia and Respiratory Infections
- Acute Myeloid Leukemia Research
- Salivary Gland Disorders and Functions
- Epigenetics and DNA Methylation
- Cancer-related Molecular Pathways
- Cancer Treatment and Pharmacology
- Cancer-related gene regulation
- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- Drug-Induced Ocular Toxicity
- Pancreatic and Hepatic Oncology Research
- RNA regulation and disease
- Acute Lymphoblastic Leukemia research
- Biochemical and Molecular Research
National Yang Ming Chiao Tung University
2007-2025
Academia Sinica
2025
Chang Gung University
2015-2025
Chang Gung Memorial Hospital
2021-2025
Linkou Chang Gung Memorial Hospital
2021-2024
Molecular Medicine Research Institute
2022
China Medical University
2021
Washington Hospital
2021
Medical College of Wisconsin
2017-2020
University of California, Irvine Medical Center
2019
Oral squamous cell carcinoma is a prominent cancer worldwide, particularly in Taiwan. By integrating omics analyses 50 matched samples, we uncover Taiwanese patients predominant mutation signature associated with cytidine deaminase APOBEC, which correlates the upregulation of APOBEC3A expression APOBEC3 gene cluster at 22q13. significantly higher tumors carrying APOBEC3B-deletion allele(s). High-level better overall survival, especially among alleles, as examined second cohort (n = 188; p...
Talaromyces marneffei causes life-threatening opportunistic infections, mainly in Southeast Asia and South China. T. infects patients with human immunodeficiency virus (HIV) but also individuals without known immunosuppression. Here we investigated the involvement of anti–IFN-γ autoantibodies severe infections HIV-negative patients. We enrolled 58 adults who were otherwise healthy. found a high prevalence neutralizing (94.8%) this cohort. The presence was strongly associated HLA-DRB1*16:02...
Oral cavity squamous cell carcinoma (OSCC) is one of the most common cancers worldwide. In Taiwan, OSCC fifth leading cause cancer-related mortality and leads to 2800 deaths per year. The poor outcome patients principally ascribed fact that this disease often advanced at time diagnosis, suggesting early detection urgently needed. Analysis body fluids promising approach identify biomarker candidates cancers. To biomarkers, salivary proteomes patients, individuals with oral potentially...
Abstract TP53 mutations are recognized to correlate with a worse prognosis in individuals non-small cell lung cancer (NSCLC). There exists an immediate necessity pinpoint selective treatment for patients carrying mutations. Potential drugs were identified by comparing drug sensitivity differences, represented the half-maximal inhibitory concentration (IC50), between mutant and wild-type NSCLC lines using database analysis. In addition, clinical data from collected evaluate both their status...
Despite their lack of protein-coding potential, long noncoding RNAs (lncRNAs) and circular (circRNAs) have emerged as key determinants in gene regulation, acting to fine-tune transcriptional signaling output. These RNA transcripts are known affect expression messenger (mRNAs) via epigenetic post-transcriptional regulation. Given widespread target spectrum, well extensive modes action, a complete understanding biological relevance will depend on integrative analyses systems data at various...
Chromatin is a dynamic but highly regulated structure. DNA-binding proteins such as transcription factors, epigenetic and chromatin modifiers are responsible for regulating specific gene expression pattern may result in different phenotypes. To reveal the identity of associated with region on DNA, immunoprecipitation (ChIP) most widely used technique. ChIP assay followed by next generation sequencing (ChIP-seq) or microarray (ChIP-chip) often to study patterns protein-binding profiles cell...
Along with the constant improvement in high-throughput sequencing technology, an increasing number of transcriptome projects are carried out organisms without decoded genome information and even on environmental biological samples. To study functions novel transcripts, very first task is to identify their potential functions. We present a web-based annotation tool, FunctionAnnotator, which offers comprehensive annotations, including GO term assignment, enzyme annotation, domain/motif...
Oral squamous cell carcinoma (OSCC) accounts for >90% of cases oral cancer, including cancer at the lip and cavity oropharynx. Most OSCCs develop from potentially malignant disorders (OPMDs), which consist heterogeneous lesions with different transformation potentials that make early detection OSCC a challenge. Using targeted mass spectrometry-based assay to compare multiple candidate proteins, we previously identified matrix metalloproteinase-1 (MMP-1) as one most promising salivary...
Mycoplasma pneumoniae is currently the most commonly detected bacterial cause of childhood community-acquired pneumonia in several countries. Of note, clonal expansion macrolide-resistant ST3 occurred Japan and South Korea. An alarming surge macrolide resistance complicates treatment pneumonia. We aimed to evaluate clinical manifestation relatedness M. circulating among children Taiwan.We prospectively enrolled 626 with radiologically confirmed between 2017 2019. infection was suspected on...
Oral cavity squamous cell carcinoma (OSCC), a leading subtype of head and neck cancer, has high global incidence mortality rates. Despite surgery radiochemotherapy, about one-third patients relapse. Targeted immunotherapy options for recurrent OSCC show limited effectiveness, underscoring the need improved treatments. We generated multi-omics datasets from pretreatment samples (n=137) to identify A3A EGFR as markers precise patient stratification. Survival outcomes were assessed by comparing...
Abstract Background Omicron poses significant concerns, leading to two severe complications, encephalitis and croup in children. Neutrophil predominance lymphopenia were observed cases. We propose a distinctive blood RNA signature characterize COVID-19's immune landscape, potentially aiding differentiating from mild cases pediatric patients, with implications for clinical management. Volcano plot of differentially expressed genes between disease Fold change was the ratio average expression...
Streptococcus anginosus is a common isolate of the oral cavity and an opportunistic pathogen for systemic infections. Although pyogenic infections caused by S. are similar to those pyogenes , lacks most well-characterized virulence factors . To investigate pathogenicity we analysed genome newly identified strain, KH1, which was associated with toxic shock-like syndrome in immunocompetent adolescent. The KH1 contains nine genomic islands, two Clustered Regularly Interspaced Short Palindromic...
ABSTRACT Strains of Vibrio vulnificus , a marine bacterial species pathogenic for humans and eels, are divided into three biotypes, those virulent eels classified as biotype 2. All 2 strains possess one or more plasmids, which have been shown to harbor the 2-specific DNA sequences. In this study we determined sequences plasmids: pR99 (68.4 kbp) in strain CECT4999 pC4602-1 (56.6 kb) pC4602-2 (66.9 CECT4602. Plasmid showed 92% sequence identity with pR99. Curing from resulted loss resistance...
The clinicopathological significance of altered SWI/SNF complex has not been well evaluated in gastric cancer (GC). We examined SMARCA2, SMARCA4, SMARCB1 and ARID1A expression by immunohistochemistry 1224 surgically resected GCs with subtyping into Epstein-Barr virus (EBV), microsatellite instability (MSI) non-EBV/MSI Lauren histotypes. mutations were investigated using the GC dataset TCGA Pan-Cancer Atlas. Clinicopathological association was assessed statistical analysis. There 427 cases...
Recent findings have implicated long noncoding RNAs (lncRNAs) as pivotal gene regulators for diverse biological processes, despite their lack of protein-coding capabilities. Accumulating evidence suggests the significance lncRNAs in mediating cell signaling pathways, especially those associated with tumorigenesis. Consequently, emerged novel functional and indicators cancer development malignancy. transcriptomic profiling has recognized a tumor-biased expressed lncRNA, HOXA10-AS transcript,...
Colorectal cancer (CRC) is a major cause of mortality and morbidity. Despite advances in chemotherapy targeted therapy, unsustainable clinical benefit was noted due to recurrence therapy resistance. The immune status the patient may affect effectiveness disease treatments. dynamic change T-cell receptor (TCR) repertoire might be parameter for monitoring treatment responses. In this study, we aimed determine characteristics significance TCR patients with unresectable metastatic colorectal...
Summary Cellular senescence is a permanent proliferative arrest triggered by genome instability or aberrant growth stresses, acting as protective even tumor‐suppressive mechanism. While several key aspects of gene regulation have been known to program this cessation cell growth, the involvement epigenetic has just emerged but remains largely unresolved. Using systems approach that based on targeted profiling, we uncovered and novel chromatin modifiers with putative link senescent state...
Processing of the eukaryotic transcriptome is a dynamic regulatory mechanism that confers genetic diversity, and splicing adenosine to inosine (A-to-I) RNA editing are well-characterized examples such processing. Growing evidence reveals cross-talk between editing, but there paucity substantial for its mechanistic details contribution in physiological context. Here, our findings demonstrate tumor-associated differential conjunction with machinery, regulates expression variants HNRPLL, gene...
Oral cavity squamous cell carcinomas (OSCCs) are aggressive tumors, and their recurrence leads to poor prognosis reduced survival rates. This study aimed identify therapeutic targets evaluate the efficacy of targeted inhibitors in OSCC patient-derived xenograft (PDX) models. Herein, we reported that PDXs recapitulated genomic signatures paired primary tumors expression CHEK1, PIK3CA, PIK3CD was significantly upregulated OSCC. The antitumor CHK1 (PF477736, AZD7762, LY2606368) PI3K (BYL719,...
Molecular composition of circulating small extracellular vesicles (EVs) does not merely reflect the cells origin, but also is enriched in specific biomolecules directly associated with cellular transformation. However, while most currently identified EV-miRs are only geared towards one-dimensional disease detection, their application for long-term tracking and treatment response monitoring has been largely elusive. We established optimized a rapid, sensitive robust liquid biopsy sampling...