A5045338514- MicroRNA in disease regulation
- Hydraulic Fracturing and Reservoir Analysis
- Oral Health Pathology and Treatment
- Marine Ecology and Invasive Species
- Developmental Biology and Gene Regulation
- Salivary Gland Tumors Diagnosis and Treatment
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Echinoderm biology and ecology
- Autophagy in Disease and Therapy
- Phagocytosis and Immune Regulation
- Chromosomal and Genetic Variations
- Kruppel-like factors research
- Congenital heart defects research
- Drilling and Well Engineering
- Berberine and alkaloids research
- Reservoir Engineering and Simulation Methods
- Invertebrate Immune Response Mechanisms
- Chemical Reactions and Isotopes
- Cancer-related gene regulation
- Head and Neck Cancer Studies
- Cancer-related molecular mechanisms research
- Salivary Gland Disorders and Functions
- Ear and Head Tumors
- Liver physiology and pathology
Institute of Cellular and Organismic Biology, Academia Sinica
2017-2025
Chi Mei Medical Center
2011-2024
Academia Sinica
2017-2023
University of Geneva
2022
Tri-Service General Hospital
2021
National Defense Medical Center
2021
Neurological Surgery
2017
University of California, San Francisco
2017
National Taiwan Ocean University
2011-2017
Yanbian University
2013
Significance Oral squamous cell carcinoma (OSCC) accounts for 90% of oral cancers, and earlier detection efficiently increases the survival rate. Here, we used a comprehensive literature review to select candidates LC-multiple reaction monitoring-MS qualitatively quantitatively measure target proteins in saliva samples from individuals at-risk population Taiwan. Statistical analysis results establishes four-protein panel sufficient detect 88.6% early-stage patients 91.6% all with 80.4%...
Deuterostomes are a monophyletic group of animals that includes Hemichordata, Echinodermata (together called Ambulacraria), and Chordata. The diversity deuterostome body plans has made it challenging to reconstruct their ancestral condition decipher the genetic changes drove diversification lineages. Here, we generate chromosome-level genome assemblies 2 hemichordate species, Ptychodera flava Schizocardium californicum , use comparative genomic approaches infer chromosomal architecture...
Human and fly FoxP homologs are well-known for their roles in the development of cognitive abilities. These findings have led to hypothesis that ancestral function was neural circuits. However, complex brains human evolved independently, similar may thus be interpreted as a result convergent evolution. In addition, four gnathostome paralogs also possess diverse developmental functions unrelated neurodevelopment, which might been overlooked comparative studies invertebrate homologs. To...
Emerging research findings suggest that long non-coding RNAs (lncRNAs) are key regulators to fibrosis formation. Nevertheless, the role of lncRNA GAS5-AS1 in progression precancerous oral submucous (OSF) remains be elucidated. Quantitative real-time PCR were used examine expression OSF tissues. The activities myofibroblasts, including collagen contractility and cell migration, as well marker α-smooth muscle actin (SMA) assessed following overexpression GAS5-AS1. Also, we analyzed Smad...
Cancer recurrence and chemoresistance are two major obstacles to the treatment of oral squamous cell carcinomas (OSCC). And cancer stem cells (CSCs) have been found possess tumor initiating, self-renewal metastasis abilities, resulting in relapse OSCC. In present study, we investigated anti-CSCs effect berberine, a phenanthrene alkaloid isolated from Berberis genus. Our results demonstrated that berberine dose dependently downregulated oncogenicity vitro, including ALDH1 activity, property,...
Abstract A series of “molecular domestication” events are thought to have converted an invertebrate RAG-like (RAGL) transposase into the RAG1–RAG2 (RAG) recombinase, a critical enzyme for adaptive immunity in jawed vertebrates. The timing and order these not well understood, part because dearth information regarding RAGL-A transposon family. In contrast abundant divergent RAGL-B family, most closely resembles RAG is represented by single orphan RAG1-like (RAG1L) gene genome hemichordate...
Background Mouse krüppel-like factor 4 (Klf4) is a zinc finger-containing transcription required for terminal differentiation of goblet cells in the colon. However, studies using either Klf4−/− mice or with conditionally deleted Klf4 their gastric epithelia showed different results role epithelial cell proliferation. We used zebrafish as model organism to gain further understanding intestinal proliferation and differentiation. Methodology/Principal Findings characterized function klf4a,...
SignificanceIn this manuscript, we address an essential question in developmental and evolutionary biology: How have changes gene regulatory networks contributed to the invertebrate-to-vertebrate transition? To issue, perturbed four signaling pathways critical for body plan formation cephalochordate amphioxus zebrafish compared effects of such perturbations on expression regulation both species. Our data reveal that many genes gained response these vertebrate lineage. Moreover, show...
Areca nut (AN) is a popular carcinogen used by about 0.6-1.2 billion people worldwide. Although AN contains apoptosis-inducing ingredients, we previously demonstrated that both extract (ANE) and its 30-100 kDa fraction (ANE 30-100K) predominantly induce autophagic cell death in normal malignant cells. In this study, further explored the action mechanism of ANE 30-100K-induced autophagy (AIA) Jurkat T lymphocytes carcinoma lines including OECM-1 (mouth), CE81T/VGH (esophagus), SCC25 (tongue),...
Synopsis Metameric somites are a novel character of chordates with unclear evolutionary origins. In the early branching chordate amphioxus, anterior derived from paraxial mesodermal cells that bud off archenteron (i.e., enterocoely) at end gastrulation. Development requires fibroblast growth factor (FGF) signaling, and distinct somite compartments express orthologs vertebrate nonaxial markers. Thus, it has been proposed amphioxus homologous to head mesoderm, lateral plate mesoderm. To trace...
Gene regulatory networks underlying cellular pluripotency are controlled by a core circuitry of transcription factors in mammals, including POU5F1. However, the evolutionary origin and transformation pluripotency-related transcriptional have not been elucidated deuterostomes. PR domain-containing protein 14 (PRDM14) is specifically expressed pluripotent cells germ cells, required for establishing embryonic stem (ESCs) primordial mice. Here, we compared functions expression patterns PRDM14...
Deuterostomes are one major group of bilaterians composed by hemichordates and echinoderms (collectively called Ambulacraria) chordates. Comparative studies between these groups can provide valuable insights into the nature last common ancestor deuterostomes that bilaterians. Indirect development hemichordates, with larval phases similar to an adult body plan anteroposterior polarity like chordates other bilaterians, makes them a suitable model for studying molecular basis among...
ABSTRACT Waterflooding operations in the Prudhoe Bay field result temperature reductions of up to 130 °F within flooded region. As a result, fracture gradient is significantly reduced, and injection pressures may have be reduced avoid formation parting. The phenomena fracturing resulting from thermally induced stress reduction was investigated provide guidance for decisions on waterflood operating strategy. Thermal reservoir simulation performed determine in-si tu pressure distributions...
Although vertebrates are bilaterally symmetric organisms, their internal organs distributed asymmetrically along a left-right axis. Disruption of axis asymmetric patterning often occurs in human genetic disorders. In zebrafish embryos, Kupffer's vesicle, like the mouse node, breaks symmetry by inducing expression Nodal-related gene, spaw, left lateral plate mesoderm (LPM). Spaw then stimulates transcription itself and downstream genes, including lft1, lft2, pitx2, specifically side...
The decrease in the number of satellite cells (SCs), contributing to myofibre formation and reconstitution, their proliferative capacity, leads muscle loss, a condition known as sarcopenia. Resistance training can prevent loss; however, underlying mechanisms resistance effects on SCs are not well understood. We therefore conducted comprehensive transcriptome analysis mouse model.We compared differentially expressed genes young mice (eight weeks old), middle-aged (48-week-old) with...
Oral cancer is a prevalent malignancy affecting men globally. This study aimed to investigate the regulatory role of miR-34a in oral cells through Axl/Akt/glycogen synthase kinase-3β (GSK-3β) pathway and its impact on cellular malignancy.
The coordinated migration of bilateral cardiomyocytes and the formation cardiac cone are essential for heart tube formation. We investigated gene regulatory mechanisms involved in myocardial migration, regulation timing zebrafish embryos. Through screening treated with ethylnitrosourea, we isolated a mutant hypomorphic allele mil (s1pr2)/edg5, called s1pr2as10 (as10). Mutant embryos this expressed less mil/edg5 mRNA exhibited cardia bifida prior to 28 hours post-fertilization. Although...
Studies in various animals have shown that asymmetrically localized maternal transcripts play important roles axial patterning and cell fate specification early embryos. However, comprehensive analyses of the transcriptomes with spatial information are scarce limited to a handful model organisms. In cephalochordates (amphioxus), an branching chordate group, germline determinants form compact granule is inherited by single blastomere during cleavage stages. Further separation experiments...
Teleosts live in aquatic habitats, where they encounter ionic and acid-base fluctuations as well infectious pathogens. To protect from these external challenges, the teleost epidermis is composed of living cells, including keratinocytes ionocytes that maintain body fluid homeostasis, mucous cells secret mucus. While ionocyte progenitors are known to be specified by Delta-Notch-mediated lateral inhibition during late gastrulation early segmentation, it remains unclear how epidermal (EMCs)...
Oral cancer is one of the most prevalent malignant tumors in Taiwan. Due to heterogeneity oral cells, five-year survival rate patients only 50%. Post-translational modifications contribute protein diversity and directly influence cell functions. The inhibitor activated signal transducer activator transcription 2 (PIAS2) known undergo post-translational modifications, yet its impact on remains unclear. PIAS2 expression was modulated by transfecting cells with a vector or knocking down using...
Oral cancer has been recognized as one of the most common malignancies worldwide and ranks fifth leading cause death in Taiwan. A variety studies have demonstrated that microRNAs are involved regulation hallmarks oral carcinogenesis. Nevertheless, effect miR-1266-5p on tumorigenesis not investigated, to mention, its functional role cancer.The upregulation SASVO3 SASM5 cells was identified by RNA-Seq examined qRT-PCR analysis. The phenotypic assays including proliferation activity, migration...