A5060987354- Influenza Virus Research Studies
- Respiratory viral infections research
- Viral Infections and Immunology Research
- Viral gastroenteritis research and epidemiology
- SARS-CoV-2 and COVID-19 Research
- RNA and protein synthesis mechanisms
- SARS-CoV-2 detection and testing
- Pneumonia and Respiratory Infections
- Animal Virus Infections Studies
- Animal Disease Management and Epidemiology
- Bacteriophages and microbial interactions
- COVID-19 Clinical Research Studies
- Bacterial Infections and Vaccines
- Virus-based gene therapy research
- Cytomegalovirus and herpesvirus research
- RNA Research and Splicing
- interferon and immune responses
- COVID-19 epidemiological studies
- Genomics and Phylogenetic Studies
- Viral Infections and Vectors
- Cervical Cancer and HPV Research
- Antibiotic Resistance in Bacteria
- Infection Control and Ventilation
- Streptococcal Infections and Treatments
- RNA modifications and cancer
Chang Gung University
2015-2025
Linkou Chang Gung Memorial Hospital
2016-2025
National Health Research Institutes
2023-2025
University of Hong Kong
2023
Zhengzhou City Hospital
2014
Peking University
2009
New York State Office for People With Developmental Disabilities
2009
Real-time reverse transcription-PCR (RT-PCR) is currently the most sensitive method to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that causes disease 2019 (COVID-19). However, correlation between detectable viral RNA and culturable virus in clinical specimens remains unclear. Here, we performed culture for 60 were confirmed be positive SARS-CoV-2 by real-time RT-PCR. The could successfully isolated from 12 throat nine nasopharyngeal swabs two sputum specimens.
Taiwan experienced two waves of imported infections with Coronavirus Disease 2019 (COVID-19). This study aimed at investigating the genomic variation severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in and compared their evolutionary trajectories global strains. We performed culture full-genome sequencing SARS-CoV-2 strains followed by phylogenetic analysis. A 382-nucleotides deletion open reading frame 8 (ORF8) was found a Taiwanese strain isolated from patient on February 4,...
We provide the first evidence for role of ACSL4 in enterovirus replication organelle formation. Moreover, both enteroviruses and coronaviruses induce ferroptosis via ACSL4. These findings establish a novel regulatory mechanism viral replication. The inhibition by inhibitors can reduce yields coronaviruses, including SARS-CoV-2, implying that ACSL4-mediated is promising therapeutic target diseases.
Background & AimsGut dysbiosis plays a role in hepatic encephalopathy (HE), while its relationship at the acute episode of overt HE (AHE), disease progression and clinical outcomes remains unclear. We aimed to identify AHE-specific microbiome association patients' outcomes.MethodsWe profiled fecal changes for cohort 62 patients with cirrhosis AHE i) before treatment, ii) 2-3 days after medication iii) months recovery, three control cohorts healthy individuals, compensated or decompensated...
Abstract Background Emergence of antibiotic resistance in bacteria is an important threat to global health. Antibiotic genes (ARGs) are some the key components define bacterial and their spread different environments. Identification ARGs, particularly from high-throughput sequencing data specimens, state-of-the-art method for comprehensively monitoring evolution. Current computational methods identify ARGs mainly rely on alignment-based sequence similarities with known ARGs. Such approaches...
Recent studies have detected mutations in the EDA gene, previously identified as causing X-linked hypohidrotic ectodermal dysplasia (XLHED), two families with non-syndromic hypodontia. Notably, all affected males both exhibited isolated oligodontia, while almost female carriers showed a milder or normal phenotype. We hypothesized that gene could be responsible for sporadic oligodontia males. In this study, we examined 15 unrelated oligodontia. Three novel (p.Ala259Glu, p. Arg289Cys, and...
Human enterovirus D68 (EV-D68) was first reported in the United States 1962; thereafter, a few cases were from 1970 to 2005, but 2 outbreaks occurred Philippines (2008) and (2014). However, little is known regarding molecular evolution of this globally reemerging virus due lack whole-genome sequences analyses. Here, all publically available including 147 full 1248 partial genomes GenBank collected compared at clade subclade level; 11 whole isolated Taiwan (TW) 2014 also added database....
Abstract Since April 2022, waves of SARS-CoV-2 Omicron variant cases have surfaced in Taiwan and spread throughout the island. Using high-throughput sequencing genome, we analyzed 2,405 PCR-positive swab samples from 2,339 persons identified BA.2.3.7 as a major lineage within recent community outbreaks Taiwan.
Enteroviruses are well known for their ability to cause neurological damage and paralysis. The model enterovirus is poliovirus (PV), the causative agent of poliomyelitis, a condition characterized by acute flaccid A related virus, 71 (EV-A71), causes similar clinical outcomes in recurrent outbreaks throughout Asia. Retrospective phylogenetic analysis has shown that recombination between circulating strains EV-A71 produces outbreak-associated which exhibit increased virulence and/or...
ABSTRACT The NS1 protein encoded by influenza A virus antagonizes the interferon response through various mechanisms, including blocking cellular mRNA maturation binding CPSF30 3′ end processing factor and/or suppressing activation of regulatory 3 (IRF3). In present study, we identified two truncated proteins that are translated from internal AUGs at positions 235 and 241 open reading frame. We analyzed localization function N-truncated strains, Udorn/72/H3N2 (Ud) Puerto Rico/8/34/H1N1...
The circulation and the genomic evolution of influenza A(H3N2) viruses during 2021/2022 2022/2023 seasons were studied associated with infection outcomes.
An avian influenza A H7N9 virus emerged in March 2013 and caused a remarkable number of human fatalities. Genome variability these viruses may provide insights into host adaptability. We scanned over 140 genomes the isolated from humans identified 104 positions that exhibited seven or more amino acid substitutions. Approximately half substitutions were ribonucleoprotein (RNP) complex. Although PB2 627K promotes replication humans, 45 147 investigated sequences retained E signature at this...
It has been observed that variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are more stable and infectious after storage at −20°C than 4°C. A SARS-CoV-2 S-D614G variant is currently the most dominant in circulation associated with enhanced infectivity.
Herein, we present a new approach for serological testing SARS-CoV-2 antibodies using innovative laboratory methods that demonstrate combination of biology and mathematics. The traditional virus neutralization test is the gold standard method; however, it time-consuming poses risk to medical personnel.
Abstract Enteroviruses (EVs), single‐stranded, positive‐sense RNA viruses, can be classified into four species (A−D), which have previously been linked to a diverse range of disease manifestations and infections affecting the central nervous system. In Enterovirus B (EV‐B), Echovirus type 11 (E11) has observed occasionally circulate in Taiwan, was responsible for an epidemic enterovirus 2018. Here, 48 clinical specimens isolated 2003, 2004, 2009, 2018 were collected high‐throughput...
Forty-two cytopathic effect (CPE)-positive isolates were collected from 2008 to 2012. All could not be identified for known viral pathogens by routine diagnostic assays. They pooled into 8 groups of 5–6 reduce the sequencing cost. Next-generation (NGS) was conducted each group mixed samples, and proposed data analysis pipeline used identify in these samples. Polymerase chain reaction (PCR) or enzyme-linked immunosorbent assay (ELISA) individually 42 depending on predicted types group. Two...
Abstract Autoantibodies against interferon-gamma (AutoAbs-IFN-γ) can cause the immunodeficiency condition following various opportunistic infections. Gut microbiota affect human immune system in many ways. Many studies have shown that gut dysbiosis was associated with some diseases, such as autoimmune diseases and virus (HIV) infection, while its relationship at anti-IFN-γ AAbs remains unknown. We aimed to identify specific microbiome possible association immunodeficiency. profiled fecal for...
Healthcare-associated COVID-19 infections caused by SARS-CoV-2 have increased morbidity and mortality. Hospitals skilled nursing facilities (SNFs) been challenged infection control management.This case study presents an outbreak investigation in a COVID-19-designated hospital hospital-based SNF. Real-time polymerase chain reaction (PCR) other studies were performed on samples obtained from SNF residents, patients, healthcare workers (HCWs). The results of the laboratory tests field...
The influenza A virus contains 8 segmented genomic RNAs and was considered to encode 10 viral proteins until investigators identified the 11th protein, PB1-F2, which uses an alternative reading frame of PB1 gene. recently PB1-N40, PA-N155 PA-N182 have shown potential for using a leaking ribosomal scanning mechanism generate novel open frames (ORFs). These ORFs provide examples manner in expands its coding capacity by overlapping frames. In this study, we performed computational search, based...
Abstract Incidence of invasive pneumococcal disease caused by antimicrobial-resistant Streptococcus pneumoniae types not included in conjugate vaccines has increased, including a penicillin- and meropenem-resistant serotype 15A-ST63 clone Japan. During 2013–2017, we collected 206 isolates Taiwan for penicillin meropenem susceptibility testing. We found serotypes 15B/C-ST83 were the most prevalent clones. A transformation study confirmed that penicillin-binding protein (PBP) 2b was primary...
Enterovirus A71 (EV-A71) is an important nonpolio enterovirus that causes severe neurological complications. In 1998, Taiwan experienced EV-A71 outbreak caused 78 deaths. Since then, periodic epidemics of associated with newly emerging strains have occurred. Several these are known to be recombinant; however, how arose within such a short period time remains unknown. Here, we sequenced 64 full-length genomes from clinical isolates collected 2005 2016 and incorporated all 91 Taiwanese...