A5026894870- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Circular RNAs in diseases
- Cell death mechanisms and regulation
- Venomous Animal Envenomation and Studies
- Mycobacterium research and diagnosis
- Ion channel regulation and function
- RNA modifications and cancer
- Autophagy in Disease and Therapy
- Colorectal Cancer Treatments and Studies
- PI3K/AKT/mTOR signaling in cancer
- Ginseng Biological Effects and Applications
- Quinazolinone synthesis and applications
- Pesticide Exposure and Toxicity
- Glioma Diagnosis and Treatment
- Cellular transport and secretion
- Cancer Immunotherapy and Biomarkers
- Retinoids in leukemia and cellular processes
- S100 Proteins and Annexins
- Atherosclerosis and Cardiovascular Diseases
- RNA Research and Splicing
- Corneal surgery and disorders
- Hops Chemistry and Applications
- Bioactive Compounds and Antitumor Agents
- Chronic Myeloid Leukemia Treatments
Taipei Medical University
2015-2024
Indiana University School of Medicine
2017
National Sun Yat-sen University
2003-2014
Kaohsiung Medical University
1999-2013
Kaohsiung Medical University Chung-Ho Memorial Hospital
2011-2013
MicroRNAs (miRNAs), small noncoding RNAs, can control gene expression by binding to their target genes for degradation and/or translational repression. Epigenetic mechanisms are defined as heritable changes in that do not involve coding sequence modifications. Both play an important role maintaining physiological functions and also related disease development. However, few studies report miRNA-mediated epigenetic regulations involved atherosclerosis. In the present study, oxidized...
Proliferation and migration of vascular smooth muscle cells (VSMCs) can cause atherosclerosis neointimal formation. MicroRNAs have been shown to regulate cell proliferation phenotype transformation. We discovered abundant expression microRNA-195 in VSMCs conducted a series studies identify its function the cardiovascular system. MicroRNA-195 was initially found be altered when were treated with oxidized low-density lipoprotein (oxLDL) non-replicated microRNA array experiment. Using cellular...
Lectin-like oxidized LDL receptor-1 (LOX-1) is a surface scavenger receptor for low-density lipoprotein (oxLDL). Several transcription factors have been reported to regulate LOX-1 expression. MicroRNAs are small noncoding RNAs that control gene expression, but there no reports of expression being regulated by microRNAs. Because the microRNA let-7g has predicted bind mRNA, we investigated whether can Our experiments first demonstrated oxLDL reduce We later confirmed binding site on...
Colorectal cancer (CRC) is associated with high recurrence and mortality. Because deregulation of microRNAs CRC development recurrence, the expression levels can be a simple reliable biomarker to detect postoperative early relapse, thereby helping physicians treat high-risk patients more efficiently. We used microRNA arrays observed that microRNA-93 had substantially different in (recurrence within 12 months after surgery) non-early relapse patients. The replication study, which included 35...
High UBE2S in malignant cancers contributes to cell motility through EMT signaling and is reversed by luteolin quercetin.
Background Estrogen receptor α (ERα) has been shown to protect against atherosclerosis. Methylation of the ERα gene can reduce expression leading a higher risk for cardiovascular disease. Recently, microRNAs have found regulate DNA methyltransferases (DNMTs) and thus control methylation status in several genes. We first searched involved DNMT-associated gene. also tested whether statin traditional Chinese medicine (San-Huang-Xie-Xin-Tang, SHXXT) could exert therapeutic effect on microRNA,...
Flavonoids are well-known antioxidants and have shown the ability to prevent tumor formation recurrence. Especially in dietary flavonoids, they provided convenience consistence of intake for long-term prevention formation. Previous reports suggested that S100 calcium-binding protein A7 (S100A7) might activate epithelial-mesenchymal transition (EMT) signaling promote metastasis cells; however, regulatory was unclear. In this study, we found S100A7 highly expressed cancer cells could be...
Flavonoids luteolin and quercetin can inhibit growth metastasis of cancer cells. In our previous report, was shown to block Akt/mTOR/c-Myc signaling. Here, we found reduced protein level transactivation activity RPS19 in A431-III cells, which is isolated from parental A431 (A431-P) cell line. Further investigation the inhibitory mechanism on RPS19, c-Myc binding sites promoter. The Akt inhibitor LY294002, mTOR rapamycin 10058-F4 significantly suppressed expression activities. Overexpression...
We showed previously that single nucleotide polymorphism (SNP) rs662702 in PAX6 may be located a microRNA-328 binding site causes susceptibility to high myopia. Our study was done elucidate the role of and its relationship with myopia.A luciferase assay used confirm locus. Clones containing each allele were created tested for their affinity microRNA-328. Because low level is risk factor myopia, we whether knockdown affects retinal pigment epithelial (RPE) cells scleral cells, as well...
// Wen-Ying Lee 1, 2 , Wai-Theng 3 Chia-Hsiung Cheng 3, 4 Ku-Chung Chen Chih-Ming Chou Chu-Hung Chung 5 Min-Siou Sun Hung-Wei Meng-Ni Ho Cheng-Wei Lin 1 Department of Pathology, Chi Mei Medical Center, Tainan, Taiwan School Medicine, College Taipei University, Taipei, Biochemistry and Molecular Cell Biology, Graduate Institute Sciences, Cellular Organismic Academia Sinica, Correspondence to: Lin, e-mail: cwlin@tmu.edu.tw Keywords: chlorpromazine, drug reposition, p53, apoptosis, SIRT1...
Temozolomide (TMZ), an alkylating agent of the imidazotetrazine series, is a first-line chemotherapeutic drug used in clinical therapy glioblastoma multiforme, most common and high-grade primary glioma adults. Micro (mi)RNAs, which are small noncoding RNAs, post-transcriptionally regulate gene expressions involved gliomagenesis. However, no studies have reported relationships between TMZ miRNA regulation. We investigated TMZ-mediated profiles its molecular mechanisms underlying induction...
Abstract Background Long noncoding (lnc)RNAs and glycolysis are both recognized as key regulators of cancers. Some lncRNAs also reportedly involved in regulating metabolism. However, glycolysis-associated lncRNA signatures their clinical relevance cancers remain unclear. We investigated the roles Methods Glycolysis scores were established using a single-sample gene set enrichment analysis (GSEA) The Cancer Genome Atlas pan-cancer data. Consensus clustering assays genomic classifiers used to...
Purpose.: The paired box 6 (PAX6) is involved in eye development and associated with several ocular diseases. Conflicting results have been reported regarding the association between PAX6 polymorphism myopia. This case–control study functional assay were conducted to identified a risk for Methods.: cohort included 1083 cases (≤ −6.0 D) 1096 controls (≥ −1.5 from Chinese population residing Taiwan. Four common tag single-nucleotide polymorphisms (SNPs) an SNP at 3′ untranslated region (UTR)...
Platelet-derived growth factor (PDGF) can promote vascular smooth muscle cells (VSMCs) to switch from the quiescent contractile phenotype synthetic phenotype, which contributes atherosclerosis. We aimed investigate role of microRNA let-7g in phenotypic switching. Bioinformatics prediction was used find target genes PDGF/mitogen-activated protein kinase 1 (MEKK1)/extracellular signal-regulated (ERK)/Krüppel-like factor-4 (KLF4) signalling pathway that affects VSMC The luciferase reporter...
Hyperlipidemia, including the oxidized low-density lipoprotein (oxLDL) accumulation, is a risk and highly associated with development of cancers cardiovascular diseases. microRNA-210 (miR-210), hypoxia-responsive microRNA regulated by HIF-1α, has been implicated in cancer disease formation. Furthermore, Bioinformatics analysis revealed that promoter miR-210 gene contains CpG-rich regions. It unclear whether expression could be epigenetically these progresses. The study aimed to explore...