A5058724362- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- HIV/AIDS Research and Interventions
- Viral Infections and Immunology Research
- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- RNA Research and Splicing
- Semantic Web and Ontologies
- HIV, Drug Use, Sexual Risk
- Biomedical Text Mining and Ontologies
- Antiplatelet Therapy and Cardiovascular Diseases
- SARS-CoV-2 and COVID-19 Research
- Influenza Virus Research Studies
- Acute Myocardial Infarction Research
- Bacteriophages and microbial interactions
- COVID-19 Clinical Research Studies
- Galectins and Cancer Biology
- Glycosylation and Glycoproteins Research
- Stock Market Forecasting Methods
- Pancreatic and Hepatic Oncology Research
- Microbial infections and disease research
- Animal Disease Management and Epidemiology
- Complex Systems and Time Series Analysis
- Cytomegalovirus and herpesvirus research
- Pancreatitis Pathology and Treatment
University of Colorado Anschutz Medical Campus
2022-2025
National Chiayi University
2023-2025
Mackay Memorial Hospital
2009-2025
University of Pittsburgh Medical Center
2025
Kaohsiung Chang Gung Memorial Hospital
2023
Chang Gung University
2023
National Cancer Institute
2020-2023
China Medical University
2022-2023
China Medical University
2023
China Medical University Hospital
2023
Curcuminoids, the predominant polyphenolic compounds in rhizome of Curcuma longa Linn., consist curcumin, demethoxycurcumin (DMC), and bisdemethoxycurcumin (BDMC). They exhibit multiple desirable characteristics for a neuroprotective agent including antioxidant, anti-inflammatory, antiamyloid activities. In this work, we report first investigation neurotrophic action mechanism curcuminoids PC12 cells, which respond to nerve growth factor (NGF) therefore serve as model system primary neuronal...
Abstract Cellular proteins CPSF6, NUP153 and SEC24C play crucial roles in HIV-1 infection. While weak interactions of short phenylalanine-glycine (FG) containing peptides with isolated capsid hexamers have been characterized, how these cellular factors functionally engage biologically relevant mature lattices is unknown. Here we show that prion-like low complexity regions (LCRs) enable avid binding to lattices. Structural studies revealed multivalent CPSF6 assembly mediated by LCR-LCR...
Abstract The movement of viruses and other large macromolecular cargo through nuclear pore complexes (NPCs) is poorly understood. human immunodeficiency virus type 1 (HIV-1) provides an attractive model to interrogate this process. HIV-1 capsid (CA), the chief structural component viral core, a critical determinant in transport virus. interactions with NPCs are dependent on CA, which makes direct contact nucleoporins (Nups). Here we identify Nup35, Nup153, POM121 coordinately support entry....
Human myxovirus resistance 2 (MX2/MXB) is an interferon-induced GTPase that inhibits human immunodeficiency virus-1 (HIV-1) infection by preventing nuclear import of the viral preintegration complex. The HIV-1 capsid (CA) major determinant for sensitivity to MX2, and complex interactions between CA, nucleoporins (Nups), cyclophilin A (CypA), other cellular proteins influence outcome infection. To explore CypA, we utilized a CRISPR-Cas9/AAV approach generate CypA knock-out cell lines as well...
ABSTRACT Interlinked interactions between the viral capsid (CA), nucleoporins (Nups), and antiviral protein myxovirus resistance 2 (MX2/MXB) influence human immunodeficiency virus 1 (HIV-1) nuclear entry outcome of infection. Although RANBP2/NUP358 has been repeatedly identified as a critical player in HIV-1 import MX2 activity, mechanism by which RANBP2 facilitates infection is not well understood. To explore MX2, CA, RANBP2, we utilized CRISPR-Cas9 to generate cell lines expressing from...
Colorectal cancer (CRC) is associated with high recurrence and mortality. Because deregulation of microRNAs CRC development recurrence, the expression levels can be a simple reliable biomarker to detect postoperative early relapse, thereby helping physicians treat high-risk patients more efficiently. We used microRNA arrays observed that microRNA-93 had substantially different in (recurrence within 12 months after surgery) non-early relapse patients. The replication study, which included 35...
Abstract Pancreatic cancer is one of the most lethal and chemo-resistant cancers worldwide. Growing evidence supports theory that gut microbiota plays an essential role in modulating host response to anti-cancer therapy. The present study aimed explore effect probiotics as adjuvant during chemotherapy for pancreatic cancer. An LSL-Kras G12D/− -Pdx-1-Cre mouse model ductal adenocarcinoma (PDAC) was created effects using four-week multi-strain ( Lactobacillus paracasei GMNL-133 reuteri...
Accumulating evidence suggests that there is a link between the host microbiome and pancreatic carcinogenesis, Porphyromonas gingivalis (P. gingivalis) increases risk of developing cancer. The aim current study was to clarify role P. in pathogenesis cancer potential immune modulatory effects probiotics. six-week-old LSL-K-rasG12D; Pdx-1-cre (KC) mice smeared on gums, causing intraepithelial neoplasia (PanIN) after four weeks be similar extent lesions untreated KC at 24 weeks. oral...
Bladder cancer ranks as the 9th most common type of worldwide. Approximately 70 % bladder cancers are diagnosed non-muscle invasive, and they treated with transurethral resection followed by intravesical therapy. Doxorubicin is one effective cytotoxic drugs used in systemic therapy, but its cardiotoxicity nephrotoxicity limit therapeutic dosages. Vorinostat an anticancer drug to treat cutaneous T-cell lymphoma, it demonstrates potent combination effects various treatments. We aim investigate...
Oral administration is the most common mode of medical treatment for pediatric patients. Although over 98% patients in targeted unit require oral medication, adherence rate 2022 was 43.9%. The reasons this low were identified as: (1) no relevant educational materials, (2) continued nursing education provided, and (3) lack assistive tools feeding aids to help administer medication young children. This project developed increase preschool inpatients our hospital. improvement strategies...
Lenacapavir (LEN) is a highly potent, long-acting antiretroviral medication for treating people infected with muti-drug-resistant HIV-1 phenotypes. The inhibitor targets multifaceted functions of the viral capsid protein (CA) during replication. Previous studies have mainly focused on elucidating LEN’s mode action ingress. Additionally, has been shown to interfere mature assembly egress. However, mechanism how LEN affects maturation unknown. Here, we show that pharmacologically relevant...
Pasteurella multocida causes progressive atrophic rhinitis and suppurative bronchopneumonia in pigs, which results severe economic losses swine industry. This study aimed to determine the serovar, genotype prevalence of toxA virulence gene isolates collected Taiwan. A total 164 from 161 diseased pigs were characterized by serotyping, multilocus sequence typing (MLST), antimicrobial susceptibility testing, presence (toxA) antibiotic resistance (floR). The majority strains serovar D:L6 (48.2%;...
Enterovirus 71 (EV71), a causative agent of hand, foot, and mouth disease can be classified into three genotypes many subtypes. The objectives this study were to conduct molecular epidemiological EV71 in the central region Taiwan from 2002-2012 test hypothesis that whether alternative appearance different subtypes is due transmission neighboring countries or re-emergence pre-existing local strains. We selected 174 isolates used reverse transcription-polymerase chain reaction amplify their...
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S) glycoprotein D614G mutation became the predominant globally circulating variant after its emergence in early disease 2019 (COVID-19) pandemic. Studies showed that this results an open conformation of S receptor-binding domain (RBD), and increased angiotensin 1-converting enzyme (ACE2) binding fusion, which result increase SARS-CoV-2 transmissibility infectivity. Dynamic tracking Europe North America, but not China....
Abstract Streptococcus suis ( S. ) infection can cause clinically severe meningitis, arthritis, pneumonia and septicemia in pigs. To date, studies on the serotypes, genotypes antimicrobial susceptibility of affected pigs Taiwan are rare. In this study, we comprehensively characterized 388 isolates from 355 diseased Taiwan. The most prevalent serotypes were 3, 7 8. Multilocus sequence typing (MLST) revealed 22 novel types (STs) including ST1831-1852 one new clonal complex (CC), CC1832....
Glaesserella parasuis (G. parasuis) belongs to the normal microbiota of upper respiratory tract in swine, but virulent strains can cause systemic infections commonly known as Glässer's disease that leads significant economic loss swine industry. Fifteen serotypes G. have been classified by gel immunodiffusion test while molecular serotyping based on variation within capsule loci further improved serotype determination unidentified field strains. Serovar has used an indicator virulence;...
The circulating recombinant form (CRF) 07_BC is the most prevalent HIV-1 strain among injection drug users (IDUs) in Taiwan. It contains a 7 amino-acid deletion its p6gag. We conducted cohort study to compare viral loads and CD4 cell count changes between patients infected with subtype B CRF07_BC elucidate mechanism. Twenty-one 59 were selected from of 667 HIV-1/AIDS whom have been followed up for 3 years. Generalized estimated equation was used analyze their clinical data results showed...
Type I interferons (IFNs) represent an essential innate defense mechanism for controlling enterovirus 71 (EV 71) infection. Mice inoculated with EV produced a significantly lower amount of type IFNs than those poly (I:C), adenovirus V, or coxsackievirus B3 (CB3). infection, however, mounted proinflammatory response significant increase in the levels serum and brain interleukin (IL)-6, monocyte chemoattractant protein-1, tumor necrosis factor, IFN-γ. infection abolished both (I:C)-...
The number of men who have sex with (MSM) infected HIV-1 in Taiwan has increased rapidly the past few years. goal this study was to conduct a molecular epidemiological infection among MSM identify risk factors for intervention. Voluntary counseling program and anonymous testing were provided patrons at 1 gay bar, 7 night clubs 3 saunas Taipei New Cities 2012. subtypes determined using gag subtype-specific PCR phylogenetic analysis by env sequences. Recent LAg-Avidity EIA. In-depth interviews...