A5068469818- Microtubule and mitosis dynamics
- Endoplasmic Reticulum Stress and Disease
- DNA Repair Mechanisms
- Genomics and Rare Diseases
- RNA modifications and cancer
- Bioactive Compounds and Antitumor Agents
- Congenital heart defects research
- Cardiac Valve Diseases and Treatments
- Genomics, phytochemicals, and oxidative stress
- Genetic factors in colorectal cancer
- Cardiomyopathy and Myosin Studies
- Nanopore and Nanochannel Transport Studies
- BRCA gene mutations in cancer
- Erythrocyte Function and Pathophysiology
- Advanced Proteomics Techniques and Applications
- Pharmacological Effects and Assays
- Hormonal and reproductive studies
- Genetics and Neurodevelopmental Disorders
- Mitochondrial Function and Pathology
- RNA Research and Splicing
- Extracellular vesicles in disease
- RNA and protein synthesis mechanisms
- Estrogen and related hormone effects
- Mass Spectrometry Techniques and Applications
- Ubiquitin and proteasome pathways
Centenary Institute
2022-2024
The University of Sydney
2023-2024
Chang Gung University
2009-2015
Chang Gung Memorial Hospital
2009-2015
National Cheng Kung University
2009
National Yang Ming Chiao Tung University
2009
University of California, Los Angeles
2004-2009
University of California, Berkeley
1999-2000
Abstract Tetrahydrogestrinone (18a‐homo‐pregna‐4,9,11‐trien‐17 β ‐ol‐3‐one or THG) was identified in the residue of a spent syringe that had allegedly contained an anabolic steroid undetectable by sport doping control urine tests. THG synthesized hydrogenation gestrinone and characterized mass spectrometry NMR spectroscopy. We developed evaluated sensitive specific methods for rapid screening samples liquid chromatography/tandem (LC/MS/MS) underivatized (using transitions m/z 313 to 241 159)...
Nasopharyngeal carcinoma (NPC) is one of the most common malignant tumors in Southeast Asia. Unfortunately, NPC victims have had metastasis when first diagnosed due to its deep location and vague symptoms. To date, discovery sensitive specific biomarkers for improving detection remains a challenge. Our previous study established strategy cell secretome analysis using hollow fiber culture (HFC) system combined with liquid chromatography mass spectrometry. Herein, above platform was used...
Variants which disrupt splicing are a frequent cause of rare disease that have been under-ascertained clinically. Accurate and efficient methods to predict variant's impact on needed interpret the growing number variants unknown significance (VUS) identified by exome genome sequencing. Here, we present results CAGI6 Splicing VUS challenge, invited predictions 56 ascertained clinically functionally validated determine impact. The performance 12 prediction methods, along with SpliceAI CADD,...
Cuproptosis, an emerging cell death pathway, offers alternative approach for antimicrobial therapy, but it suffers from deficiencies and health risks. Here, we design hollow Cu-enriched Prussian blue-based nanostructures (Cu-HMPBs) find that the infected microenvironment facilitates release of Cu ions Cu-HMPBs, leading to overload in bacterial cells. Meanwhile, Fe cells are highly selectively chelated, triggering iron starvation. As a result, proteotoxic stress redox imbalance induced by...
Indole-3-carbinol (I3C), a component of Brassica vegetables, is under study as preventive agent cancers the breast and other organs. Following ingestion, I3C converted to series oligomeric products that presumably are responsible for in vivo effects I3C. We report major trimeric product, 5,6,11,12,17,18-hexahydrocyclonona[1,2-b:4,5-b':7,8-b' ']triindole (CTr), on estrogen receptor (ER) signaling pathways. Tumor-promoting high doses may be due activation aryl hydrocarbon (AhR)-mediated...
The Australian Genomics Cardiovascular Disorders Flagship was a national multidisciplinary collaboration. It aimed to investigate the feasibility of genome sequencing (GS) and functional genomics resolve variants uncertain significance (VUS) in clinical management patients families with cardiomyopathies, primary arrhythmias, congenital heart disease (CHD).
Protein phosphorylation is a vital post-translational modification that involved in variety of biological processes. Several mass spectrometry-based methods have been developed for phosphoprotein characterization. In our previous work, we demonstrated the capability computational algorithm mining phosphopeptide signals large LC−MS data sets by measuring shifts due to phosphatase treatment (Wu, H. Y.; Tseng, V. S.; Liao, P. C. J. Proteome Res. 2007, 6, 1812−1821). Mass accuracy seems play an...
Centrosomal protein 55 (Cep55), located in the centrosome interphase cells and recruited to midbody during cytokinesis, is essential for completion of cell abscission. Northern blot previously showed that a high level Cep55 predominantly expressed testis. In present study, we examined spatial temporal expression patterns mouse testis maturation. We found Cep55, together with pericentrin, another centrosomal protein, were localized intercellular bridges (IBs) interconnecting spermatogenic...
TBX20 encodes a cardiac transcription factor that is associated with atrial septal defects. Recent studies implicate loss-of-function variants left ventricular non-compaction cardiomyopathy (LVNC), although clinical and genetic data in families are limited. We report four segregate LVNC. Genetic testing using genome or exome sequencing was performed index cases, were validated Sanger sequencing, cascade family members. A multi-exon deletion, small essential splice site variant nonsense found...
Formulae display:?Mathematical formulae have been encoded as MathML and are displayed in this HTML version using MathJax order to improve their display. Uncheck the box turn off. This feature requires Javascript. Click on a formula zoom.
Abstract Background Variants which disrupt splicing are a frequent cause of rare disease that have been under-ascertained clinically. Accurate and efficient methods to predict variant’s impact on needed interpret the growing number variants unknown significance (VUS) identified by exome genome sequencing. Here we present results CAGI6 Splicing VUS challenge, invited predictions 56 ascertained clinically functionally validated determine impact. Results The performance 12 prediction methods,...