Background: Carpal tunnel syndrome (CTS), with unclear etiology, is the most common entrapment neuropathy. Its occurrence related to lots of medical and non‐medical conditions uncertain causality. With a large population, we characterized selected demographical clinical factors add more information on CTS‐correlated new insight into future CTS prevention. Methods: A national insurance claim dataset one million enrollees in Taiwan was used identify 15 802 patients 31 604 randomly controls,...

Abstract Regulation of fatty acid uptake, lipid production and storage, metabolism droplets (LDs), is closely related to homeostasis, adipocyte hypertrophy obesity. We report here that stomatin, a major constituent raft, participates in adipogenesis maturation by modulating signaling pathways. In adipocyte-like cells, increased stomatin promotes LD growth or enlargements facilitating LD-LD fusion. It also uptake from extracellular environment recruiting effector molecules, such as FAT/CD36...

The 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1) is a potential regulatory node in the mevalonate pathway that frequently dysregulated tumors. This study found HMGCS1 expression upregulated stomach adenocarcinoma samples of patients and tumorspheres gastric cancer cells. elevates levels pluripotency genes Oct4 SOX-2 contributes to tumorsphere formation ability also promotes vitro cell growth progression vivo tumor lung metastasis After blocking by statin dipyridamole, exerts...

// Chin-Hui Lin 1 , Yao-Wen Ying-Chun Chen Chen-Chung Liao 2 Yuh-Shan Jou 3 Ming-Ta Hsu Chian-Feng VYM Genome Research Center, National Yang-Ming University, Taipei, Taiwan Proteomics Institutes of Biomedical Sciences, Academia Sinica, Correspondence to: Chen, email: cfchen@ym.edu.tw Keywords: FNDC3B, hepatocellular carcinoma, metastasis Received: April 05, 2016 Accepted: June 13, Published: July 01, ABSTRACT Recurrence and are common in carcinoma (HCC) correlate with poor prognosis. We...

Protein O-GlcNAcylation is a dynamic post-translational modification involving the attachment of N-acetylglucosamine (GlcNAc) to hydroxyl groups Ser/Thr residues on numerous nucleocytoplasmic proteins. Two enzymes are responsible for O-GlcNAc cycling substrate proteins: transferase (OGT) catalyzes addition while O-GlcNAcase (OGA) helps removal GlcNAc. modifies protein functions; therefore, dysregulation affects cell physiology and contributes pathogenesis. To maintain homeostasis cellular...

Purpose: The alteration of the exosomal proteins in aqueous humor (AH) is linked to development eye diseases. goal this study was examine protein profile patients with age-related macular degeneration (AMD) better understand their role pathogenesis AMD. Methods: Exosomes were isolated from AH 28 AMD and 25 control eyes. quality, concentration, size distribution exosomes measured using a nanoparticle tracking analysis system (NTA). Total each sample purified digested trypsin for liquid...

O-GlcNAcylation is a reversible and dynamic post-translational protein modification catalyzed by O-GlcNAc transferase (OGT). Despite the reported association of with cancer metastasis, proteome profile for aggressiveness remains largely uncharacterized. Here, we report our comparative profiling two differentially invasive lung adenocarcinoma cell lines, which identified 158 down-regulated 106 up-regulated candidates in highly cells. Among these differential proteins, nuclear RNA-binding...

Type-2 diabetes mellitus (T2DM)-induced sarcopenia is intertwined with diminished insulin sensitivity and extracellular matrix (ECM) remodeling in skeletal muscle other organs. Physical activities such as aerobic exercise play a crucial role regulating blood glucose levels, sensitivity, metabolic pathways, oxidative stress, fibrosis, ECM remodeling, regeneration by modulating differentially expressed protein (DEP) levels. The objectives of our research were to investigate the effect six...

Summary Skeletal muscle has emerged as one of the most important tissues involved in regulating systemic metabolism. The gastrocnemius is a powerful skeletal composed predominantly glycolytic fast‐twitch fibers that are preferentially lost among old age. This decrease mass remarkable during aging; however, underlying molecular mechanism not fully understood. Strikingly, there ~70% Cisd2 protein, key regulator lifespan mice and disease gene for Wolfram syndrome 2 humans, within after middle...

Globally, the proportion of older adults is increasing. Older people face chronic conditions such as sarcopenia and functional decline, which are often associated with disability frailty. Proteomics assay potential serum biomarkers frailty in adults. were divided into non-frail frail groups (n = 6 each; 3 males each group) accordance Chinese-Canadian Study Health Aging Clinical Frailty Scale. Adults measured for grip power 6-min walk test physical activity, venous blood was sampled after...

The dynamic behavior of a holographic grating induced in homeotropically aligned dye-doped liquid-crystal film is investigated. In the presence an applied dc voltage, photoexcited azo dyes induce photorefractive and then diffuse are adsorbed onto cell substrates. reorientation liquid crystals as result leads to phase that shifted 90° from grating. Competition these two gratings induces two-beam coupling writing beams, initially transferring energy beam 1 2 then, after pause, 1.

Abstract Background: The prognostic factors of intracerebral haemorrhage (ICH) in haemodialysis (HD) patients are not fully clear and there is no standard clinical grading scale to predict 30‐day mortality. Our aim was develop such a scale. Methods: Records all HD with spontaneous ICH presenting Chang Gung Memorial Hospital Taiwan during 1994–2004 were reviewed. study design retrospective analysis data collected from one hospital. Prognostic identified by Student’s t ‐test χ 2 ‐test....

Saliva is considered as the best source of biological material for biomarker discovery studies since it noninvasive in comparison to other body sources. Usually buffalo cannot precisely express estrus signals. Hence, there a need concise methods detect time ensure success artificial insemination. Therefore, we have established reference proteome map on whole saliva during their estrous cycle with special estrus. Nearly 12 bands been observed using sodium dodecyl sulfate polyacrylamide gel...

Although non‑alcoholic fatty liver disease (NAFLD) is considered a benign disorder, hepatic steatosis has been proposed to be involved in the tumorigenesis of cancer. However, underlying mechanism for carcinogenesis diseases remains unclear. Cancer stem cells (CSCs) have hypothesized serve key role tumorigenesis. Tumor formation begins with subset heterogeneous that share properties cells, such as self‑renewal and undifferentiated properties. Our previous study reported saturated acid...

Abstract A large number of mammalian odorant‐binding proteins, which are lipocalins, have been studied. These proteins participate in peri‐receptor events by selecting and carrying odorant molecules. The present study aimed at identifying the buffalo salivary protein (sOBP), to determine its post‐translational modification using mass spectrometry. 21 kDa was initially separated adopting sodium dodecyl sulfate‐polyacrylamide gel electrophoresis it identified as sOBP with high statistical...

Protein phosphorylation is a vital post-translational modification that involved in variety of biological processes. Several mass spectrometry-based methods have been developed for phosphoprotein characterization. In our previous work, we demonstrated the capability computational algorithm mining phosphopeptide signals large LC−MS data sets by measuring shifts due to phosphatase treatment (Wu, H. Y.; Tseng, V. S.; Liao, P. C. J. Proteome Res. 2007, 6, 1812−1821). Mass accuracy seems play an...

The role of pheromones and pheromone-binding proteins in the laboratory rat has been extensively investigated. However, we have previously reported that preputial gland Indian commensal produces a variety pheromonal molecules glands would seem to be predominant source for communication. presence not yet identified rat; therefore, experiments were designed unravel α2u-globulin (α2u) its bound volatiles rat. Total glandular first fractionated by sodium dodecyl sulfate/polyacrylamide gel...

Postmenopausal women exhibit a higher prevalence of obesity due to decreased energy expenditure and increased food intake compared their premenopausal counterparts. Brown adipose tissue (BAT) plays key role in homeostasis, thus providing us with appealing therapeutic targets obesity. However, how BAT proteomes are altered response low levels estrogen remains unclear. To better understand the underlying mechanisms between postmenopausal state proteomic changes, our study aimed investigate...

Sarcopenic obesity is characterized by excess fat mass and diminished muscular mass/function. DNAJA3, a mitochondrial co-chaperone protein, plays crucial role in skeletal muscle development. GMI, an immunomodulatory promotes myogenic differentiation through DNAJA3 activation. This study aims to elucidate the physiological effects of Dnaja3 haploinsufficiency on dysfunction dysregulated lipid metabolism assess efficacy GMI rescuing sarcopenic both vitro vivo.

TWK10 (LP10) is a probiotic known to improve endurance exercise performance. Here, we analyze the proteomics and metagenomic changes in LP10 supplemented mouse model. Male ICR mice were divided into two groups (

The effects of light-induced reorientation on a homeotropical dye-doped liquid crystal (DDLC) cell are discussed. photoexcited azo dye Methyl Red (MR) is diffused and adsorbed onto the substrate, thus forming ripple structure. laser-induced structure then reorient liquid-crystal molecules induce holographic grating. Initially, directors reoriented primarily by dye. However, given sufficiently large groove amplitude, torque imposed grooves overcomes that which due to dyes, crystals realigned...

Cataracts are one of the most common eye diseases that can cause blindness. Discovering susceptibility factors in proteome contribute to cataract development would be helpful gaining new insights molecular mechanisms process. We used label-free nanoflow ultra-high-performance liquid chromatography–tandem mass spectrometry compare aqueous humor protein expressions patients with different risk such as diabetes mellitus (DM) and smoking controls (with cataract) without exposure. Eight who...

Atherosclerosis is the preliminary cause of coronary artery disease, one diseases that account for largest number fatal mortalities. Physical activity an effective strategy to restrain atherosclerosis from deterioration. Evidence indicated changes in proteomic profile are highly associated with development, but mechanism behind exercise amelioration has not yet been investigated a proteomics perspective. Hence, profiles could further elucidate systematic effects intervention on ApoE knockout...

We have developed two kinds of ultra-flexible film-like liquid crystal display, pixel-isolated twist nematic and polymer encapsulated displays. By the aids wall in thin variation cell gap bending condition is greatly reduced. They are good candidates for rollable flexible display due to excellent flexibility. In this work, first time that electro-optical properties reliability a after test investigated, application limit investigated.