Abstract The aim of the present work is to biosynthesize Chitosan nanoparticles (CTNp) using tea ( Camellia sinensis) extract, with potent antimicrobial properties towards phytopathogens rice. Preliminary chemical analysis extract showed that they contain carbohydrate as major compound and uronic acid indicating nature acidic polysaccharide. structure isolated polysaccharide was analyzed through FTIR 1 H NMR. CTNp prepared by addition polysaccharides chitosan solution. size determined DLS...

Cervico-vaginal fluid (CVF) plays significant roles in coitus, sperm transport, and implantation. It is believed to be a good noninvasive biomarker for various diagnostic purposes. In this study, comprehensive proteomic analysis of buffalo CVF was performed during the estrous cycle order document protein expressions, utilizing SDS-PAGE, mass spectrometry, immunoblot. The main objective screen one or more estrus-specific proteins. A total 416 proteins were identified both estrus diestrus...

Saliva is considered as the best source of biological material for biomarker discovery studies since it noninvasive in comparison to other body sources. Usually buffalo cannot precisely express estrus signals. Hence, there a need concise methods detect time ensure success artificial insemination. Therefore, we have established reference proteome map on whole saliva during their estrous cycle with special estrus. Nearly 12 bands been observed using sodium dodecyl sulfate polyacrylamide gel...

Male urinary lipocalin family proteins, practically odorant-binding proteins but also could be pheromones by themselves, in rodents act as a shuttle for chemosignal communication and facilitate delivery of the signals access to congeners. However, presence this protein urine female has not yet been reported. Therefore, present investigation was carried out find if is house rat and, so, whether its expression differs between phases estrous cycle. The separated single dimensional gel...

<b>Context:</b> Predicting ovulation is the basis on which fertile period determined. Nowadays there are many methods available to detect ovulatory period. Unfortunately, these not always effective for accurate detection of ovulation. Hence, an attempt was made through single dimension sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) analysis protein with help saliva ferning.<br> <b>Aims:</b> The aim this study determine association level endogenous reproductive hormone...

Pheromones are odoriferous volatile chemical cues produced by animals for communication among conspecifics so as to regulate their social behaviors. In general, the odor compounds recognized receptors in nasal cavity. Odorant-binding protein (OBP), a lipocalin family protein, mediates air-borne through mucus. The presence of OBP several mammalian species is well documented but to-date there no report buffalo. Hence, present study was undertaken investigate if buffalo Uni- and two-dimensional...

Novel drug delivery carriers such as liposomes are very versatile to suit the of various molecules. Clindamycin is generally considered most effective antibiotic in treatment acne. Topical clindamycin used less often due lower efficacy or increased side effects. Thus, have been selected for present work assuming that incorporation into may reduce effects associated with it. To overcome potential risk adverse and resistance from prescription medications, traditional herbal medicines...

Assessment of salivary volatile compounds adopting gas chromatography-linked mass spectrometry (GC-MS) analysis revealed the presence a total 11 in buffalo saliva irrespective stages reproductive cycle. p-cresol was identified as an estrus-specific compound saliva. In addition, modeling odorant-binding protein (OBP) and β-lactoglobulin that OBP is highly stable has strong binding affinity with p-cresol. Hydrogen bond interactions indicated responsible for pheromone release through contrast,...

Abstract Transportation of pheromones bound with carrier proteins belonging to lipocalin superfamily is known prolong chemo-signal communication between individuals the same species. Members family (MLF) have three structurally conserved motifs for delivery hydrophobic molecules specific recognizer. However, computational analyses are critically required validate and emphasize sequence structural annotation MLF. This study focused elucidate evolution, documentation, stability binding...

In view of the silent nature estrus in buffalo, a noninvasive assay kit has long been felt necessary for easy and effective detection. This study was designed to detect buffalo using formulated our laboratory based on pheromone compound. Group I: Urine samples collected at phase group II: randomly urine were subjected test kit. No colour developed (i.e., positive reaction) after adding solution. By contrast, pale and/or dark pink negative from proestrus diestrus buffaloes, respectively....

Introduction: The architecture of dental plaque biofilms provides a resistant environment for bacteria. Chemical control agents containing antimicrobials (chlorhexidine, herbal mouthwash) are adjunctively used to halt the growth plaque. In order overcome adverse effects that result due extensive use synthetic formulation, several preparations have been tested their effectiveness. current study, chlorhexidine (CHX) and mouthwash in vitro on planktonic sessile phases selected strains putative...

Aim: The aim was to investigate the association between cortisol and dehydroepiandrosterone (DHEA) levels in patients with periodontitis healthy controls. Materials Methods: Cortisol DHEA saliva were determined 20 subjects, clinical examinations including oral hygiene index, sulcus bleeding index (Muhlemann Son) probing depth also performed. Statistical Analysis: Data analyzed help of SPSS software package (version 7.0), significance level set at 95% confidence interval. Mann-Whitney test...

<b>Background</b> : Community periodontal index of treatment needs (CPITN) is commonly used to measure disease. It's uniqueness, apart from assessing the status, also gives for underlying condition. Benzoyl-DL-arginine napthylamide (BANA) test a chair side diagnostic detect presence putative pathogens. We correlated CPITN scores patients with BANA results assess validity as an indicator anaerobic infection.<br><b> Objectives</b> The present study was aimed correlate activity subgingival...

Ovulation is such a critical physiological process that its noninvasive detection based on salivary constituents has several advantages in humans. Hence, the present study proposed to identify ovulatory-specific proteins saliva order detect ovulation phase.Samples were collected from women volunteers. The procedure adopted was approved by Institutional Human Ethical Committee (DM/2014/101/38), Bharathidasan University. samples thirty healthy female volunteers, with prior written consent....

Abstract Silent oestrus is an unsurmountable problem in the management of buffalo reproduction. In addressing this issue, we have earlier reported variation levels urinary luteinizing hormone (LH) through different phases oestrous cycle with extended window during mid‐oestrous phase. Based on report, present study designed to assess salivary LH cycle. Bovine ELISA kit was used determine level LH. We observed a notable The maximum level, 39.07 mIU/ml, oestrus, which significantly ( p &lt;...

Background: Since periodontal diseases are primarily inflammatory in nature, the ability to detect lesions gingival tissue is essential for diagnosis and monitoring of changes status. Gingival bleeding has been used as a reliable objective clinical parameter not only evaluation conditions periodontium, but also detection its activity progression. The index (Loe) based on presence or absence bleeding, thereby provides means converting observed data into statistical analysis. Periodontitis...

Male urinary lipocalin family proteins, practically odorant-binding proteins but also could be pheromones by themselves, in rodents act as a shuttle for chemosignal communication and facilitate delivery of the signals access to congeners.However, presence this protein urine female has not yet been reported.Therefore, present investigation was carried out find if is house rat and, so, whether its expression differs between phases estrous cycle.The separated single dimensional gel...

<b>Context:</b> Chronic periodontitis is an inflammatory condition of supporting tissues initiated by organisms in dental plaque. The reactive oxygen species and free radicals mediate connective tissue destruction periodontitis. In order to counteract the radical mediated damage, numerous antioxidant mechanisms exist within host. One such system heme oxygenase enzymes. Heme key enzyme involved catabolism heme. It cleaves molecule yield equimolar amounts biliverdin, carbon monoxide, iron....