A5066970526- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- RNA Research and Splicing
- RNA Interference and Gene Delivery
- DNA Repair Mechanisms
- Cancer-related gene regulation
- RNA and protein synthesis mechanisms
- Head and Neck Cancer Studies
- Oral Health Pathology and Treatment
- Genomics, phytochemicals, and oxidative stress
- Molecular Biology Techniques and Applications
- Viral-associated cancers and disorders
- MicroRNA in disease regulation
- Lymphoma Diagnosis and Treatment
- Lung Cancer Treatments and Mutations
- Histone Deacetylase Inhibitors Research
- Bioactive Natural Diterpenoids Research
- Liver Disease Diagnosis and Treatment
- Natural product bioactivities and synthesis
- Pharmacological Effects of Natural Compounds
- Sarcoma Diagnosis and Treatment
- Hepatitis B Virus Studies
- Radiomics and Machine Learning in Medical Imaging
- Epigenetics and DNA Methylation
- DNA and Nucleic Acid Chemistry
Chang Gung Memorial Hospital
2015-2024
Chang Gung University of Science and Technology
2016-2024
Chang Gung University
2021-2024
Taoyuan Chang Gung Memorial Hospital
2020-2024
Linkou Chang Gung Memorial Hospital
2024
ORCID
2021
Memorial Hospital of South Bend
2012-2020
TD Bank
2019
National Yang Ming Chiao Tung University
2007-2008
Taipei Veterans General Hospital
2007-2008
Abstract Particulate matter 2.5 (PM2.5) is a risk factor for lung cancer. In this study, we investigated the molecular mechanisms of PM2.5 exposure on cancer progression. We found that short‐term to 24 h activated EGFR pathway in cells (EGFR wild‐type and mutant), while long‐term 90 days persistently promoted activation, cell proliferation, anchorage‐independent growth, tumor growth xenograft mouse model EGFR‐driven H1975 cells. showed AhR translocate into nucleus activation. further...
// Tong-Hong Wang 1 , Yong-Shiang Lin 2 Ying Chen Chau-Ting Yeh 3 Yen-lin Huang Tsung-Han Hsieh Tzong-Ming Shieh 4 Chuen Hsueh 1, Tse-Ching Tissue Bank, Chang Gung Memorial Hospital, Tao-Yuan, Taiwan Department of Anatomic Pathology, University School Medicine, Hepato-Gastroenterology, Liver Research Center, Dental Hygiene, College Health Care, China Medical University, Taichung, Correspondence to: Chuen-Hsueh, e-mail: ch9211@adm.cgmh.org.tw Chen, ctc323@adm.cgmh.org.tw Keywords: long...
The aim of the present study was to determine antibacterial activities phenolic essential oil (EO) compounds hinokitiol, carvacrol, thymol, and menthol against oral pathogens. Aggregatibacter actinomycetemcomitans, Streptococcus mutans, Methicillin-resistant Staphylococcus aureus (MRSA), Escherichia. coli were used in this study. minimum inhibitory concentrations (MICs), bactericidal (MBCs), bacterial growth curves, temperature pH stabilities, synergistic effects liquid vapor EO tested....
EphrinA5, a member of the ephrinA subclass, is downregulated in hepatocellular carcinoma (HCC) and acts as tumor suppressor. However, upstream regulation mechanism ephrinA5 remains unclear. In this study, we tried to identify characterize roles miR-96 miR-182 expression HCC. The levels were examined 47 paired HCC para-tumoral liver tissues using quantitative real-time RT-PCR. luciferase reporter assay western blotting employed dissect association between miR-96/182 expression. Moreover,...
Recently, increasing numbers of long noncoding RNAs (lncRNAs), with both oncogenic and tumor-suppressive potential, have been found to be aberrantly expressed in various human cancers. However, the function lncRNAs hepatocellular carcinoma (HCC) progression remains largely unknown. In this study, we performed a comprehensive microarray analysis lncRNA expression using HCC specimens. After validation 119 tissues, identified novel tumor suppressor lncRNA, CPS1 intronic transcript 1 (CPS1-IT1)....
Detection of nodal micrometastasis (tumor size: 0.2-2.0 mm) is challenging for pathologists due to the small size metastatic foci. Since lymph nodes with are counted as positive nodes, detecting crucial accurate pathologic staging colorectal cancer. Previously, deep learning algorithms developed manually annotated images performed well in identifying breast cancer sentinel nodes. However, process manual annotation labor intensive and time consuming. Multiple instance was later used identify...
Abstract In the long history of traditional Chinese medicine, single herbs and complex formulas have been suggested to increase lifespan. However, identification molecules responsible for lifespan extension has challenging. Here, we collected a list medicines with potential longevity properties from pharmacopeias. By utilizing mother enrichment program, systematically screened these identified herb, Psoralea corylifolia , that increases in Saccharomyces cerevisiae. Next, twenty-two pure...
Corylin is a flavonoid extracted from the nuts of Psoralea corylifolia L. (Fabaceae), which widely used anti-inflammatory and anticancer herb in China. Recent studies revealed antioxidant, anti-inflammatory, bone differentiation-promoting effects corylin. However, there are no examining activity In this study, we cells animal models to examine antitumor corylin on hepatocellular carcinoma (HCC) then studied its downstream regulatory mechanisms. The results showed that significantly inhibited...
Hinokitiol displays potent antimicrobial activity. It has been used in toothpaste and oral-care gel to improve the oral lichen planus reduce halitosis. The aim of this study was evaluate activity 3 different dental root canal sealers with hinokitiol (sealers+H) their physical biological effects. AH Plus (epoxy amine resin-based, AH), Apexit (calcium-hydroxide-based, AP), Canals (zinc-oxide-eugenol-based, CA), were study. original CA exhibited strong anti-methicillin-resistant Staphylococcus...
Isoliquiritigenin (ISL), a natural compound extracted from licorice, has chemopreventive and antitumor activities. The purpose of this study was to investigate the anticancer effect ISL on human oral squamous cell carcinoma (OSCC).The anti-OSCC effects were evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide test, flow cytometry, reverse transcription-polymerase chain reaction, Western blotting, promoter activity, terminal deoxynucleotidyl transferase dUTP nick-end...
Patients with triple-negative breast cancer have few therapeutic strategy options. In this study, we investigated the effect of isoliquiritigenin (ISL) on proliferation cells. We found that treatment ISL inhibited cell line (MDA-MB-231) growth and increased cytotoxicity. reduced cycle progression through reduction cyclin D1 protein expression sub-G1 phase population. The ISL-induced apoptotic population was observed by flow cytometry analysis. Bcl-2 treatment, whereas Bax level increased;...
Honokiol and magnolol are natural components isolated from Magnolia bark that is used in traditional Chinese Japanese herbal medicine. These two isomers as a component of dietary supplements cosmetic products. In this study, we investigated the antimicrobial effect honokiol on pathogens causing oral diseases, their mechanism action biofilm formation drug resistance pathogens, inflammatory regulation mammalian cells. We determined minimum inhibitory concentration bactericidal magnolol,...
Abstract Corylin, a biologically active agent extracted from Psoralea corylifolia L. (Fabaceae), promotes bone differentiation and inhibits inflammation. Currently, few reports have addressed the biological functions that are regulated by corylin, to date, no studies investigated its antitumor activity. In this study, we used cell functional assays analyze activity of corylin in hepatocellular carcinoma (HCC). Furthermore, whole-transcriptome were performed identify downstream genes...
Pathologic diagnosis of nasopharyngeal carcinoma (NPC) can be challenging since most cases are nonkeratinizing with little differentiation and many admixed lymphocytes. Our aim was to evaluate the possibility identify NPC in biopsies using deep learning. A total 726 were included. Among them, 100 randomly selected as testing set, 20 validation all other 606 training set. All three datasets had equal numbers benign cases. Manual annotation performed. Cropped square image patches 256 × pixels...
Background The immune checkpoint blockade (ICB) targeting programmed cell death-1 (PD-1) and its ligand (PD-L1) has been proved beneficial for numerous types of cancers, including non-small-cell lung cancer (NSCLC). However, a significant number patients with NSCLC still fail to respond ICB due unfavorable tumor microenvironment. To improve the efficacy, immune-chemotherapy combination pemetrexed, cis/carboplatin pembrolizumab (anti-PD-1) recently approved as first-line treatment in advanced...
Epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors (TKIs) are approved treatments for non-small-cell lung cancer (NSCLC) patients harboring activating EGFR mutations. The C797S mutation is one of the known acquired-resistance mutations to latest third-generation TKIs. At present, there no clear options treating who acquire resistance acquisition was shown upregulate expression AXL, a TAM (TYRO3-AXL-MER) family, and suppression AXL effective in reducing NSCLC cells C797S. As...
Abstract Magnolol, a hydroxylated biphenol compound isolated from the bark of Magnolia officinalis , has been shown to exhibit anti-proliferative effect in various cancer cells, including skin cells. Methoxylation magnolol appears improve its anti-inflammatory activity, yet this modification on agent’s antitumor activity remains unknown. In work, we report that 2-O-methylmagnolol (MM1) displays improved against cells compared both vitro and vivo . The increased MM1 correlate with ability...
DNA repair systems are abnormally active in most hepatocellular carcinoma (HCC) cells due to accumulated mutations, resulting elevated capacity and resistance chemotherapy radiotherapy. Thus, targeting mechanisms is a common treatment approach HCC sensitize cancer damage. In this study, we examined the anti-HCC effects of melatonin elucidated regulatory mechanisms. The results functional assays showed that addition inhibiting proliferation, migration, invasion abilities cells, suppressed...
Abstract Liver fibrosis assessment by biopsy and conventional staining scores is based on histopathological criteria. Variations in sample preparation the use of semi-quantitative methods commonly result discrepancies between medical centers. Thus, minor changes liver might be overlooked multi-center clinical trials, leading to statistically non-significant data. Here, we developed a computer-assisted, fully automated, staining-free method for hepatitis B-related assessment. In total, 175...
Daphne genkwa, a Chinese medicinal herb, is used frequently in Southeast Asian countries to treat diseases; the flavonoid hydroxygenkwanin (HGK) extracted from its flower buds. The bioactivity of HGK, particularly as an anti-liver cancer agent, has not been explored. In this study, human hepatocellular carcinoma (HCC) cell lines and animal xenograft model were employed investigate both activity HGK against liver cellular signaling mechanisms. HCC cells treated with subjected function assays....