MicroRNAs (miRNA) are endogenously expressed noncoding RNAs with important biological and pathological functions that yet to be fully defined. This study investigated alterations in miRNA expression head neck squamous cell carcinoma (HNSCC), the incidence of which is rising throughout world. Initial screening subsequent analysis identified a panel aberrantly miRNAs HNSCC tissues, miR-31 among most markedly upregulated. Ectopic increased oncogenic potential cells under normoxic conditions...

Abstract Background Oral carcinoma is an important malignancy throughout the world. MicroRNAs (miRNAs) are endogenously expressed, non‐coding RNAs that regulate post‐transcriptional levels of targeted mRNAs. MiRNA‐31 ( miR‐31 ) significantly upregulated in oral tissues and plays oncogenic roles carcinogenesis. Methods We analyzed saliva patients with n = 45), verrucous leukoplakia 10), control healthy individuals 24) by quantitative reverse transcriptase‐polymerase chain reaction (RT‐PCR)....

Abstract MicroRNA‐200c (miR200c) is emerging as an important regulator of tumourigenicity and cancer metastasis with a strong capacity for inducing epithelial–mesenchymal transitions. However, the role miR200c in head neck squamous cell carcinoma (HNSCC) HNSCC‐associated stem cells (HNSCC‐CSCs) unknown. In this study, expression regional metastatic lymph node HNSCC tissues was significantly decreased, but BMI1 increased compared to parental tumours. Importantly, site‐directed mutagenesis...

Abstract Head and neck squamous cell carcinoma (HNSCC) is a prevalent cancer worldwide. Signal transducers activators of transcription 3 (STAT3) signaling reported to promote tumor malignancy recurrence in HNSCC. Cucurbitacins, triterpenoid derivatives, are strong STAT3 inhibitors with anticancer properties. Recent studies have shown aldehyde dehydrogenase 1 (ALDH1) be marker stem cells (CSC) The aim this study was investigate the therapeutic effect cucurbitacin I HNSCC-derived CSCs. Using...

MicroRNAs are short non-coding RNAs that regulate gene expression and crucial to tumorigenesis. Oral squamous cell carcinoma (OSCC) is a prevalent malignancy worldwide. Up-regulation of miR-146 has been identified in OSCC tissues. However, the roles carcinogenesis controversial as it suppressive many other malignancies. The present study investigated pathogenic implications miR-146a oral carcinogenesis. Microdissected exhibits higher levels than matched adjacent mucosal cells. plasma...

Head and neck squamous cell carcinoma (HNSCC) is a prevalent disease worldwide, the survival of HNSCC has not improved significantly over last few decades. MicroRNAs (miRNAs) have an important regulatory role during carcinogenesis. Our study investigated pathogenic implications miR-134 in head The clinicopathologic were using expression assays functional pathogenesis was determined ectopic expression, knockdown reporter assay experiments. Xenographic tumorigenesis orthotopic nodal metastasis...

Phase change materials are widely considered for application in nonvolatile memories because of their ability to achieve phase transformation the nanosecond time scale. However, knowledge fast crystallization dynamics these is limited lack and accurate temperature control methods. In this work, we have developed an experimental methodology that enables ultrafast characterization phase-change on a more technologically relevant melt-quenched amorphous using practical device structures. We...

miR-211 expression in human oral squamous cell carcinoma (OSCC) has been implicated poor patient survival. To investigate the oncogenic roles of miR-211, we generated K14-EGFP-miR-211 transgenic mice tagged with GFP. Induction carcinogenesis using 4-nitroquinoline 1-oxide (4NQO) resulted more extensive and severe tongue tumorigenesis compared control animals. We found that 4NQO arecoline upregulated OSCC cells. In silico experimental evidence further revealed directly targeted transcription...

Oral squamous cell carcinoma (OSCC) is a globally prevalent malignancy. The molecular mechanisms of this cancer are not well understood and acquire elucidation. Peroxiredoxin like 2A (PRXL2A) has been reported to be an antioxidant protein that protects cells from oxidative stress. Our previous study identified association between PRXL2A upregulation in OSCC worse patient prognosis. MicroRNAs (miRNAs) small non-coding RNAs involved the modulation biological/pathological properties. miR-125...

Abstract Oral squamous cell carcinoma (OSCC) is a common malignancy, the incidence of which particularly high in some Asian countries due to geographically linked areca quid (AQ) chewing habit. In this study, array‐based comparative genomic hybridization was used screen microdissected OSCCs for genome‐wide alterations. The highest frequencies gene gain were detected TP63, Serpine1, FGF4/FGF3 , c‐ Myc and DMD. deletion Caspase8 MTAP . Gained genes, classified by hierarchical clustering,...

MicroRNAs (miRNAs) are non-coding RNAs that play roles in gene silencing and may be involved tumorigenesis. miR-211 was mapped to chromosome 15q13, a locus frequently altered cancers. The role of miR-211in carcinogenesis has not been clearly defined, however. This study investigated the pathogenetic implications oral carcinogenesis. An association found between higher expression most advanced nodal metastasis, vascular invasion, poor prognosis carcinoma. function enforced carcinoma cells...

Oral squamous cell carcinoma (OSCC) is a prevalent malignancy worldwide. MicroRNAs are short non-coding RNAs that regulate gene expression and crucial for tumorigenesis. Previously, we have identified miR-31 frequently upregulated in OSCC this increase, together with downstream effector modulation, enhances oral carcinogenesis. We higher levels of potential malignant disorder (OPMD) tissues compared normal mucosa. Exogenous human telomerase reverse transcriptase (hTERT) were introduced into...

Background: Oral squamous cell carcinoma (OSCC) is a disease that affects patients worldwide. DNA of dead cells released into the blood stream and may be isolated from plasma or serum samples. This termed cell-free (cfDNA). cfDNA increased in several types malignancies. We investigated if there was correlation between levels progression OSCC. Methods: Using quantitative spectrometry, we measured 121 with OSCC 50 matched controls. Mann Whitney Wilcoxon tests were used to compare differences...

In recent years, the incidence and mortality rates of head neck squamous cell carcinoma (HNSCC) have increased worldwide. Therefore, understanding genomic alterations in HNSCC carcinogenesis is crucial for appropriate diagnosis therapy. Protocadherin FAT1, which encodes 4588 amino acid residues, regulates complex mechanisms to promote oncogenesis or suppression malignancies. Multiplex PCR-based next-generation sequencing (NGS) revealed FAT1 somatic mutations. The clinicopathologic...

Oral squamous cell carcinoma (OSCC) is a common malignancy worldwide. This study clarified the oncogenic role of miR-134 in OSCC. Reporter assays, using both wild-type and mutant constructs, confirmed that Programmed Cell Death 7 (PDCD7) gene was potential target miR-134. The OSCC cells exogenously expressed exhibited reduced PDCD7 expression. As expected, exogenous miRZip-134 expression increased cells; additionally, suppressed oncogenicity cells. By contrast, knockout through editing vitro...

Betel quid (BQ) chewing has been associated with an increased risk of oral squamous cell carcinoma (OSCC) and submucous fibrosis (OSF). Piper betle inflorescence, which contains 15 mg/g safrole, is a unique ingredient BQ in Taiwan. Chewing such prepared may contribute to safrole exposure human beings (420 μM saliva). Safrole known rodent hepatocarcinogen, yet its carcinogenicity largely undetermined. In this study, using 32 P-post-labeling method, we have found high frequency safrole-like...

We present a flow chart for the determination of eleven intermediates chlorophyll biosynthesis and degradation pathways. This is organized from six literatures. just add step to link two unrelated procedures determine content pheophytin b. It convenient people who want chlorophylls their derivatives. makes it possible pigments more than 100 samples in one work day.