A5004560801- Cancer therapeutics and mechanisms
- Retinoids in leukemia and cellular processes
- Arsenic contamination and mitigation
- RNA modifications and cancer
- Synthesis and Biological Evaluation
- DNA Repair Mechanisms
- Synthesis and Reactivity of Heterocycles
- Cancer-related molecular mechanisms research
- Click Chemistry and Applications
- Heme Oxygenase-1 and Carbon Monoxide
- Genomics, phytochemicals, and oxidative stress
- Epigenetics and DNA Methylation
- Cancer, Hypoxia, and Metabolism
- Ferroptosis and cancer prognosis
- Cancer-related Molecular Pathways
- Ubiquitin and proteasome pathways
- interferon and immune responses
- DNA and Nucleic Acid Chemistry
- Carcinogens and Genotoxicity Assessment
- Molecular Biology Techniques and Applications
- Alcohol Consumption and Health Effects
- Gene expression and cancer classification
- Histone Deacetylase Inhibitors Research
- Cytokine Signaling Pathways and Interactions
- Drug Transport and Resistance Mechanisms
Academia Sinica
2010-2024
Institute of Biomedical Sciences, Academia Sinica
2014-2023
National Yang Ming Chiao Tung University
2008-2019
National Taipei University of Technology
2017-2018
Institute of Molecular Biology, Academia Sinica
2011-2017
China Medical University
2017
National Yang Ming University Hospital
2014
In-Q-Tel
2011
Institute of Cellular and Organismic Biology, Academia Sinica
2007-2009
National Research Institute of Chinese Medicine
2008-2009
Abstract Personalized medical care focuses on prediction of disease risk and response to medications. To build the models, access both large-scale genomic resources human genetic studies is required. The Taiwan Biobank (TWB) has generated high-coverage, whole-genome sequencing data from 1492 individuals genome-wide SNP 103,106 Han Chinese ancestry using custom arrays. Principal components analysis genotyping showed that full range variation was found in cohort. arrays also include thousands...
Long-term arsenic exposure is associated with an increased risk of vascular diseases including ischemic heart disease, cerebrovascular and carotid atherosclerosis. The pathogenic mechanisms atherogenicity are not completely clear. A fundamental role for inflammation in atherosclerosis its complications has become appreciated recently. To investigate molecular targets inflammatory pathway possibly involved arsenic-associated atherosclerosis, we conducted exploratory study using cDNA...
Arsenic is a well-established carcinogen in humans, but there little evidence for its carcinogenicity animals and it inactive as an initiator or tumor promoter two-stage models of mice. Two arsenic salts (sodium arsenite sodium arsenate) induced high frequency methotrexate-resistant 3T6 cells, which were shown to have amplified copies the dihydrofolate reductase gene. The ability induce gene amplification may relate carcinogenic effects humans since oncogenes observed many human tumors....
Arsenic is a notorious environmental toxicant known as both carcinogen and an atherogen in human beings, but the pathogenic mechanisms are not completely understood. In cell culture studies, trivalent arsenic enhanced oxidative stress variety of mammalian cells, this association may be closely associated with development arsenic-related diseases. To investigate effect exposure on humans, we conducted population study to determine relationships blood reactive oxidants antioxidant capacity at...
Sodium arsenite and sodium arsenate were observed to induce morphological transformation of Syrian hamster embryo cells in a dose-dependent manner. A linear dose-dependence with slope ∼1 was both compounds when the data plotted on log-log graph. The trivalent > 10-fold more potent than pentavalent arsenate. also exhibited toxicity; however, at non-toxic as well toxic doses. At low doses, enhanced colony-forming efficiency observed. To understand mechanism arsenic-induced transformation,...
Cancer-initiating cells (CICs) are responsible for tumor initiation, progression, and therapeutic resistance; moreover, redox homeostasis is important in regulating cancer stemness. Previously, we have identified that containing low intracellular reactive oxygen species levels (ROSLow cells) display enhanced features of CICs. However, the specific metabolic signatures CICs remain unclear required further characterization by systemic screenings. Herein, first showed mainly relying on...
Arsenic exposure is associated with an increased risk of vascular disorders, and results in oxidative stress endothelial cells smooth muscle (VSMCs). Since involved regulating the expression genes related to atherogenesis, we investigated its involvement enhanced three atherosclerosis-related coding for heme oxygenase-1 (HO-1), monocyte chemoattractant protein-1 (MCP-1), interleukin-6 (IL-6) VSMCs treated inorganic sodium arsenite (iAs). In human (hVSMCs) rat (rVSMCs), HO-1, MCP-1, IL-6 mRNA...
The purpose of these studies was to define culture conditions that support growth and differentiation normal epithelial cells obtained from hamster tracheas. Epithelial tracheas adult hamsters were collected using enzymatic procedures cultured under various conditions. medium used consisted a 1:1 mixture 199 Dulbecco's modified Eagle's with 2% fetal bovine serum, which conditioned by mouse 3T3 before use. Insulin, transferrin, hydrocortisone, epidermal factor, an extract hypothalamus as...
Silicosis is characterized by fibrosing nodular lesions that may eventually develop into progressive massive fibrosis (PMF). Cytokines (interleukin-1beta [IL-1beta], tumor necrosis factor-alpha [TNF-alpha] and growth factors insulin-like factor-1 [IGF-1] platelet-derived factor [PDGF]) have been implicated in the formation of these lesions. TGF-beta promotes extracellular matrix accumulation upregulating collagen fibronectin gene expression, inhibits degradation decreasing secretion...
Gene inactivation through DNA hypermethylation plays a pivotal role in carcinogenesis. This study aimed to profile aberrant methylation different stages of liver disease, namely noncirrhosis, cirrhosis and hepatocellular carcinoma (HCC), also clarify the influence hepatitis B virus (HBV) infection on HCCs. Promoter p14(ARF), p16(INK4a), O(6)-methylguanine-DNA methyltransferase (MGMT), glutathione S-transferase pi (GSTP1) E-cadherin (E-Cad) genes 58 HCCs paired with adjacent nontumorous...
In the present study, we demonstrate synthesis and applications of multifunctional gold nanorod-based probes for specific targeting noninvasive imaging based on localized heating generated by nanorods after NIR irradiation. The structural design probe consists MUA (11-mercaptoundecanoic acid)-capped covalently linked with low-molecular-weight chitosan oligosaccharide (Mw ∼5000) via carbodiimide (EDC) coupling agent. This surface modification is performed complete replacement toxic CTAB...
A series of bis(hydroxymethyl)indolizino[6,7-b]indoles and their bis(alkylcarbamates) were synthesized for antitumor studies. These agents designed as hybrid molecules β-carboline (topoisomerase inhibition moiety) bis(hydroxymethyl)pyrrole (DNA cross-linking moiety). The preliminary studies indicated that these exhibited significant cytotoxicity against a variety human tumor cells in vitro. Treatment breast carcinoma MX-1 xenograft-bearing nude mice with compounds 18b 28c achieved more than...
Abstract Patients with triple-negative breast cancer (TNBC) had an increased likelihood of distant recurrence and death, as compared those non-TNBC subtype. Regorafenib is a multi-receptor tyrosine kinase (RTK) inhibitor targeting oncogenesis has been approved for metastatic colorectal advanced gastrointestinal stromal tumor. Recent studies suggest regorafenib acts SHP-1 phosphatase agonist. Here, we investigated the potential to suppress metastasis TNBC cells through SHP-1/p-STAT3/VEGF-A...
CBLC (CBL proto-oncogene c) belongs to the CBL protein family, which has E3 ubiquitin ligase activity toward activated receptor tyrosine kinases. is frequently upregulated in non-small cell lung cancer (NSCLC), yet very little known about functions of tumorigenesis. Here we show that an epigenetically demethylated target and its expression can be NSCLC after treatment with DNA methylation inhibitor 5'-azacytidine. Depletion significantly inhibited viability clonogenicity vitro reduced tumor...
Arsenic compounds are widely distributed and arsenic ingestion is associated with many human diseases, including blackfoot disease, atherosclerosis, cancers. However, the underlying mechanism of toxicity not understood. In fibroblast cells (HFW), arsenite known to induce oxidative damage, chromosome aberrations, cell cycle arrest, aneuploidy, manifestation these cellular responses dependent on changes in gene expression which can be analyzed using cDNA microarray technique. this study,...