A5003448615- Leptospirosis research and findings
- Immune Response and Inflammation
- Viral Infections and Vectors
- Veterinary medicine and infectious diseases
- Virus-based gene therapy research
- Inflammatory mediators and NSAID effects
- Genetic and Kidney Cyst Diseases
- Zoonotic diseases and public health
- Bioactive Natural Diterpenoids Research
- Cardiovascular Syncope and Autonomic Disorders
- Liver physiology and pathology
- Heart Rate Variability and Autonomic Control
- Biomarkers in Disease Mechanisms
- FOXO transcription factor regulation
- Pathogenesis and Treatment of Hiccups
- Monoclonal and Polyclonal Antibodies Research
- Renal and related cancers
- Opioid Use Disorder Treatment
- Xenotransplantation and immune response
- Phytochemical Studies and Bioactivities
- Biomedical Research and Pathophysiology
- Pharmacovigilance and Adverse Drug Reactions
- Atrial Fibrillation Management and Outcomes
- Vibrio bacteria research studies
- Cancer Mechanisms and Therapy
Wan Fang Hospital
2024
Taipei Medical University
2024
Chang Gung University
2012-2024
Chang Gung Memorial Hospital
2015-2024
Linkou Chang Gung Memorial Hospital
2022
Kaohsiung Medical University
2021-2022
Kaohsiung Medical University Chung-Ho Memorial Hospital
2021-2022
Rigel (United States)
2020
The University of Texas Medical Branch at Galveston
2018-2019
Montefiore Medical Center
2017
Background Leptospirosis is the most widespread zoonosis. Chronic human infection and asymptomatic colonization have been reported. However, renal involvement in those with leptospira chronic exposure remains undetermined. Methods Findings In 2007, a multistage sampling survey for kidney disease (CKD) was conducted southern county of Taiwan, an area high prevalence dialysis. Additionally, independent cohort 88 participants from leptospira-endemic town followed two years after flooding 2009....
Abstract Corylin, a biologically active agent extracted from Psoralea corylifolia L. (Fabaceae), promotes bone differentiation and inhibits inflammation. Currently, few reports have addressed the biological functions that are regulated by corylin, to date, no studies investigated its antitumor activity. In this study, we used cell functional assays analyze activity of corylin in hepatocellular carcinoma (HCC). Furthermore, whole-transcriptome were performed identify downstream genes...
Argininosuccinate synthetase 1 (ASS1) is a rate-limited enzyme in arginine biosynthesis. The oncogenic potential of ASS1 terms prognosis and cancer metastasis prototrophic gastric (GC) remains unclear at present. We identify differentially expressed proteins microdissected GC tumor cells relative to adjacent nontumor epithelia by isobaric mass tag for absolute quantitation proteomics analysis. with stable expression or depletion were further analyzed downstream molecules. investigated their...
Daphne genkwa, a Chinese medicinal herb, is used frequently in Southeast Asian countries to treat diseases; the flavonoid hydroxygenkwanin (HGK) extracted from its flower buds. The bioactivity of HGK, particularly as an anti-liver cancer agent, has not been explored. In this study, human hepatocellular carcinoma (HCC) cell lines and animal xenograft model were employed investigate both activity HGK against liver cellular signaling mechanisms. HCC cells treated with subjected function assays....
Prostate cancer (PCa) is one of the most common malignancies in men; recently, PCa-related mortality has increased worldwide. Although androgen deprivation therapy (ADT) standard treatment for PCa, patients often develop aggressive castration-resistant PCa (CRPC), indicating presence an alternative source androgen. Clostridium scindens a member gut microbiota and can convert cortisol to 11β-hydroxyandrostenedione (11β-OHA), which potent precursor. However, effect C. on progression not been...
Abstract Leptospirosis is an often overlooked cause of acute kidney injury that can lead to multiple organ failure and even death. The principle protein conserved in many pathogenic leptospires the outer membrane LipL32. However, role LipL32 pathogenesis renal leptospirosis not entirely clear. Here we studied effects on developing zebrafish larvae. Incubation larvae with Leptospira santarosai serovar Shermani induced tubular predominantly proximal pronephric ducts. Furthermore,...
Autophagy impairment has been demonstrated in the pathogenesis of autosomal dominant polycystic kidney disease (ADPKD) and could be a new target treatment. Trehalose is natural, nonreducing disaccharide that shown to enhance autophagy. Therefore, we investigated whether trehalose treatment reduces renal cyst formation Pkd1-hypomorphic mouse model. Pkd1 miRNA transgenic (Pkd1 miR Tg) mice wild-type littermates were given drinking water supplemented with 2% from postnatal day 35 91. The...
Abstract Proteins belonging to the toll-like receptor (TLR) family, particularly TLR2, are major components of innate immunity against Leptospira infection. The ligands for TLR2 harbor several conserved patterns such as lipidation molecules, leucine-rich repeat (LRR) domains, binding motifs, and structure. In , LipL32 interacts with on human kidney cells concomitantly stimulating inflammatory responses. However, mechanism is unknown. computational prediction suggests that β1β2, loop-α3-loop,...
Leptospira santarosai serovar Shermani is the most frequently encountered serovar, and it causes leptospirosis tubulointerstitial nephritis in Taiwan. This study aims to complete genome sequence of L. analyze transcriptional responses renal tubular cells. To assemble this highly repetitive genome, we combined reads that were generated from four next-generation sequencing platforms by using hybrid assembly approaches finish two-chromosome contiguous sequences without gaps validating data with...
Leptospirosis caused by pathogenic Leptospira spp leads to kidney damage that may progress chronic disease. However, how leptospiral infections induced renal is unclear.We apply microarray and next-generation sequencing technologies investigate the first murine transcriptome-wide, leptospires-mediated changes in gene expression identify biological pathways associated with damage.Leptospiral genes were detected transcriptomes of mice infected interrogans at day 28 postinfection, suggesting...
Metabolic reprogramming is a potential treatment strategy for autosomal dominant polycystic kidney disease (ADPKD). Metformin has been shown to inhibit the early stages of cyst formation in animal models. However, metformin can lead lactic acidosis diabetic patients with advanced chronic disease, and its efficacy ADPKD still not fully understood. Here, we investigated effect an established hypomorphic mouse model PKD that presents stable heritable knockdown Pkd1. The Pkd1 miRNA transgenic...
Approximately 1 million cases of leptospirosis, an emerging infectious zoonotic disease, are reported each year. Pathogenic Leptospira species express leucine-rich repeat (LRR) proteins that rarely expressed in non-pathogenic species. The LRR domain-containing protein family is vital for the virulence pathogenic In this study, biological mechanisms essential domain from were examined. effects and recombinant LRR20 (rLRR20) on expression levels factors involved signal transduction examined...
High-incidence regions of leptospirosis caused by Leptospira spp. coincide with chronic kidney disease. This study investigated whether asymptomatic is an emerging culprit that predisposes to progressive disease when superimposed on secondary nephrotoxic injury. Kidney histology/function and whole transcriptomic profiles were evaluated for Leptospira-infected C57/BL6 mice adenine-induced The extent tubulointerstitial lesions expression inflammation/fibrosis genes in infected low-dose (0.1%)...
Chronic kidney disease of uncertain etiology (CKDu) is an environmental nephropathy in which the etiological factors are yet uncertain. Leptospirosis, a spirochetal infection that common among agricultural communities, has been identified as potential for CKDu beyond nephropathy. Although chronic disease, endemic regions, increasing number cases reported with features suggestive acute interstitial nephritis without any known reason (AINu), or background CKD. The study hypothesizes exposure...
Liver fibrosis is reversible when treated in its early stages and liver inflammatory factors are inhibited. Limited studies have investigated the therapeutic effects of corylin, a flavonoid extracted from Psoralea corylifolia L. (Fabaceae), on fibrosis. Therefore, we evaluated anti-inflammatory activity corylin efficacy mechanism action ameliorating Corylin significantly inhibited responses by inhibiting activation mitogen-activated protein kinase signaling pathways expression interleukin...
Background: Interleukin receptor associated kinases (IRAK) 1 and 4 are involved in Toll-Like Receptor (TLR) Interleukin-1 (IL-1R) signaling pathways, which regulate innate immunity inflammation. Dysregulation of IRAK1/4 can lead to a variety inflammatory conditions including rheumatoid gouty arthritis. As result, promising therapeutic targets for rheumatic diseases (1). We have identified potent selective inhibitor, R835, that substantially suppressed the elevation LPS (TLR4 agonist)-induced...
(1) Background: The autonomic imbalance plays a role in vasovagal syncope (VVS) diagnosed by head-up tilting test (HUT). neuECG is new method of recording skin electrical signals to simultaneously analyze sympathetic nerve activity (SKNA) and electrocardiogram. We hypothesize that SKNA higher subjects with tilt-positive than tilt-negative the surges before syncope. (2) Methods: recorded 41 who received HUT (according "Italian protocol"), including rest, tilt-up, provocation recovery phases....
To estimate the direct medical costs of gastroenterological diseases within universal health insurance program among population local residents in Taiwan.The data sources were first 4 cohort datasets 200,000 people from National Health Insurance Research Database Taipei. The ambulatory, inpatient and pharmacy claims 2001 analyzed. Besides prevalence diseases, both amount utilization procedures drugs calculated.Of with 183,976 eligible people, 44.2% had ever a diagnosis during year. age group...