A5047181867- DNA Repair Mechanisms
- Telomeres, Telomerase, and Senescence
- Carcinogens and Genotoxicity Assessment
- Bacterial Genetics and Biotechnology
- DNA and Nucleic Acid Chemistry
- Cancer therapeutics and mechanisms
- RNA Research and Splicing
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- Cell death mechanisms and regulation
- Mycotoxins in Agriculture and Food
- RNA and protein synthesis mechanisms
- Digestive system and related health
- Molecular Biology Techniques and Applications
- Synthesis and Biological Evaluation
- RNA modifications and cancer
- Plant Genetic and Mutation Studies
- Cancer-related molecular mechanisms research
- Heat shock proteins research
- Cancer Research and Treatments
- RNA regulation and disease
- Photosynthetic Processes and Mechanisms
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Chromosomal and Genetic Variations
Chang Gung University
2009-2019
Chang Gung Memorial Hospital
1998-2017
Memorial Hospital of South Bend
1998
Chang Gung University of Science and Technology
1996-1997
Stanford University
1978-1989
Swiss Cancer Center Léman
1979-1981
The University of Texas at Dallas
1977
The molecular mechanisms for the recF-dependent and recB-dependent pathways of postreplication repair were studied by sedimentation analysis DNA from UV-irradiated Escherichia coli cells. When ability to daughter strand gaps was compared, uvrB recF cells showed a gross deficiency, whereas recB only small deficiency. Nevertheless, able perform some limited compared with "repairless" recA strain. introduction mutation into strain greatly increased its UV radiation sensitivity, yet decreased...
The molecular basis for the inviability of dam-3 recA200(Ts) and recB270(Ts) cells was studied. were inviable in yeast extract-nutrient broth or minimal medium at 42 degrees C. Although C, they viable C medium, which high salt content suppresses mutant phenotype caused by mutation Under growth conditions rendering dam rec inviable, accumulated double-strand breaks their DNA. Introduction a mutL mutS restored viability grown eliminated formation DNA these cells. We conclude that inability to...
Abstract Magnolol, a hydroxylated biphenol compound isolated from the bark of Magnolia officinalis , has been shown to exhibit anti-proliferative effect in various cancer cells, including skin cells. Methoxylation magnolol appears improve its anti-inflammatory activity, yet this modification on agent’s antitumor activity remains unknown. In work, we report that 2-O-methylmagnolol (MM1) displays improved against cells compared both vitro and vivo . The increased MM1 correlate with ability...
Telomerase activity is suppressed in normal human somatic tissues but activated cancer cells and immortal cell lines. The reverse transcriptase (RT) subunit telomerase (hTERT) the key regulator of activity. hTERT promoter contains E-box elements may allow upstream stimulatory factor (USF), a basic helix-loop-helix (bHLH) leucine zipper family proteins, to bind regulate expression. In this study, we investigated whether how USF effect on hTERT. Through luciferase reporter assays, found that...
The molecular defect in DNA repair caused by ssb mutations (single-strand binding protein) was studied analyzing synthesis and double-strand break production UV-irradiated Escherichia coli delta uvrB strains. presence of the ssb-113 mutation produced a large inhibition led to formation breaks, whereas ssb-1 much less fewer breaks. We suggest that single-strand protein plays an important role replication damaged DNA, it functions protecting single-stranded parental DNa opposite...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSpontaneous reactions of aflatoxin B1 modified deoxyribonucleic acid in vitroTzu-Chien V. Wang and Peter CeruttiCite this: Biochemistry 1980, 19, 8, 1692–1698Publication Date (Print):April 15, 1980Publication History Published online1 May 2002Published inissue 15 April 1980https://pubs.acs.org/doi/10.1021/bi00549a027https://doi.org/10.1021/bi00549a027research-articleACS PublicationsRequest reuse permissionsArticle Views69Altmetric-Citations31LEARN...
EGFR, a receptor tyrosine kinase (RTK), is frequently overexpressed and mutated in non-small cell lung cancer (NSCLC). Tyrosine inhibitors (TKIs) have been widely used the treatment of many cancers, including NSCLC. However, intrinsic acquired resistance to TKI remains common obstacle. One strategy that may help overcome EGFR-TKI target EGFR for degradation. As client protein heat-shock 90 (HSP90) sulforaphane known functionally regulate HSP90, we hypothesized could attenuate EGFR-related...
Telomerase, a specialized ribonucleoprotein reverse transcriptase that directs the synthesis of telomeric DNA, is repressed in normal human somatic cells, but activated most cancers. Little known concerning how telomerase activity and maintained cancer cells. We have shown previously inhibition protein kinase C (PKC) decreases nasopharyngeal carcinoma (NPC) Here, we provide evidence decrease by PKC not mediated transcriptional down-regulation hTERT, catalytic telomerase. In vitro...
A neutral filter elution assay was used to determine if the post-replicational formation and repair of DNA double-strand breaks (DSB) occurs in u.v.-irradiated human cells. Excision-deficient XP12 cells were pulse-labeled with [ 3 H]thymidine after u.v. irradiation (1.5–3 J/m 2 ), nascent followed during incubation. With increasing radiation fluences, an fraction eluted at a fast rate, indicating that DSB produced. The maximum yield observed about 24 h post-irradiation incubation 37°C....
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTFormation and excision of covalent deoxyribonucleic acid adducts benzo[a]pyrene 4,5-epoxide benzo[a]pyrenediol epoxide I in human lung cells A549George Feldman, Joyce Remsen, Tzu-Chien V. Wang, Peter CeruttiCite this: Biochemistry 1980, 19, 6, 1095–1101Publication Date (Print):March 18, 1980Publication History Published online1 May 2002Published inissue 18 March...
Natural products are the major sources of currently available anticancer drugs. We recently reported that phenanthrene-based tylophorine derivative-1 (PBT-1) may be a potential antitumor agent for lung adenocarcinoma. therefore examined direct targets PBT-1 and their effects in inhibiting found reduced level Slug inhibits migration, invasion, filopodia formation adenocarcinoma CL1-5 cells vitro. In addition, displayed vivo antimetastasis activities against subcutaneous orthotopic xenografts...
The mechanism by which recA (Srf) mutations (recA2020 and recA801) suppress the deficiency in postreplication repair shown recF mutants of Escherichia coli was studied UV-irradiated uvrB uvrA recB recC sbcB cells. partially suppressed UV radiation sensitivity recF, recB, cells, they restored ability cells to DNA daughter-strand gaps. In addition, double-strand breaks recA2020 recA801 do not appear affect synthesis radiation-induced proteins, nor produce an altered RecA protein, as detected...