A5106482293- Hepatitis B Virus Studies
- Heme Oxygenase-1 and Carbon Monoxide
- Cancer, Hypoxia, and Metabolism
- Helicobacter pylori-related gastroenterology studies
- MicroRNA in disease regulation
- Autophagy in Disease and Therapy
- SARS-CoV-2 detection and testing
- Neonatal Health and Biochemistry
- Metabolism, Diabetes, and Cancer
- SARS-CoV-2 and COVID-19 Research
- RNA Interference and Gene Delivery
- Inflammatory mediators and NSAID effects
- Systemic Lupus Erythematosus Research
- Stroke Rehabilitation and Recovery
- Amino Acid Enzymes and Metabolism
- Cerebral Palsy and Movement Disorders
- COVID-19 Clinical Research Studies
- NF-κB Signaling Pathways
- Neonatal Respiratory Health Research
- Genomics, phytochemicals, and oxidative stress
- Cancer-related molecular mechanisms research
- Fuel Cells and Related Materials
- Ginseng Biological Effects and Applications
- Mitochondrial Function and Pathology
- Sulfur Compounds in Biology
National Tsing Hua University
2018-2024
National Taiwan University Hospital
2023-2024
Asia University
2024
Chang Gung University of Science and Technology
2017-2024
Institute of Biomedical Sciences, Academia Sinica
2021
Biomedical Translation Research Center
2021
Institute of Biological Chemistry, Academia Sinica
2021
University of California, San Francisco
2017
Chang Gung University
2011-2017
National Taiwan University
2017
Glucose-6-phosphate dehydrogenase (G6PD) is the rate-limiting enzyme of pentose phosphate pathway that modulates cellular redox homeostasis via regeneration NADPH. G6PD-deficient cells have a reduced ability to induce innate immune response, thus increasing host susceptibility pathogen infections. An important part response activation inflammasome. peripheral blood mononuclear (PBMCs) from patients and human monocytic (THP-1) were used as models investigate whether G6PD inflammasome...
Entry inhibitors against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are urgently needed to control the outbreak of disease 2019 (COVID-19). This study developed a robust and straightforward assay that detected molecular interaction between receptor-binding domain (RBD) viral spike protein angiotensin-converting enzyme (ACE2) receptor in just 10 min. A drug library 1068 approved compounds was used screen for SARS-CoV2 entry inhibition, 9 active drugs were identified as...
Most cancer cells reprogram their glucose metabolic pathway from oxidative phosphorylation to aerobic glycolysis for energy production. By reducing enzyme activity of pyruvate kinase M2 (PKM2), attain a greater fraction glycolytic metabolites macromolecule synthesis needed rapid proliferation. Here we demonstrate that hydrogen sulfide (H
Abstract Glucose-6-phosphate dehydrogenase (G6PD) is a housekeeping enzyme involved in the pentose phosphate shunt for producing nicotinamide adenine dinucleotide (NADPH). Severe G6PD deficiency leads to embryonic lethality, but underlying mechanism unclear. In current study, effects of on epithelial–mesenchymal transition (EMT), especially during development, were investigated. The knockdown induced morphological changes, accompanied by suppression epithelial markers, E-cadherin and...
Abstract Background: There exists compelling evidence that some genetic variants are associated with the risk of multiple cancer sites (i.e., pleiotropy). However, biological mechanisms through which pleiotropic operate unclear. Methods: We obtained all associations from National Human Genome Research Institute-European Bioinformatics Institute GWAS Catalog, and correlated were clustered into groups. Pleiotropic variant groups genes functionally annotated. Associations noncancer traits also...
Abstract Hepatitis B virus (HBV) is an aetiological factor for liver cirrhosis and hepatocellular carcinoma (HCC). Despite current antiviral therapies that successfully reduce the viral load in patients with chronic hepatitis B, persistent surface antigen (HBsAg) remains a risk HCC. To explore whether intrahepatic antigens contribute directly to hepatocarcinogenesis, we monitored mitotic progression of HBV‐positive cells. Cytokinesis failure was increased HepG2.2.15 1.3ES2 cells, as well...
Development of effective therapeutics for mitigating the COVID-19 pandemic is a pressing global need. Neutralizing antibodies are known to be antivirals, as they can rapidly deployed prevent disease progression and accelerate patient recovery without need fully developed host immunity. Here, we report generation characterization series chimeric against receptor-binding domain (RBD) severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein. Some these exhibit exceptionally...
This study examined the relative and absolute reliability of Taiwanese version MoCA (MoCA-T) in people with stroke. The recruited 114 individuals who were at least 3 months after onset a first-ever unilateral MoCA-T was administered twice, 6-week interval, to all participants. assessed using intraclass correlation coefficient (ICC), standard error measurement (SEM), smallest real difference (SRD), SRD percentage, Bland-Altman method. ICC analysis showed highly reliable (ICC = 0.85). between...
Gastric inflammation-related disorders are commonly observed digestive system illnesses char-acterized by the activation of proinflammatory cytokines, particularly tumor necrosis factor-α (TNF-α). This results in induction cyclooxygenase-2 (COX-2)/prostaglandin E2 (PEG2) and matrix metallopeptidase-9 (MMP-9). These factors contribute to pathogenesis gastric in-flammation disorders. We examined preventive effects Lonicera japonica Thunb. ethanol ex-tract (Lj-EtOH) on...
The pathogenesis of acute respiratory distress syndrome (ARDS) is very complex. Patients with ARDS still suffer high mortality rates. Infiltration and activation neutrophils in lungs are critical pathogenic factors ARDS. In this study, we demonstrate that meso-dihydroguaiaretic acid (MDGA), a natural lignan, inhibits inflammatory responses human ameliorates mice. MDGA inhibited superoxide anion generation elastase release various G-protein coupled receptor agonists-induced neutrophils....
The regulation of matrix metalloproteinases (MMPs), especially MMP-9, has a critical role in both physiological and pathological events the central nervous system (CNS). MMP-9 is an indicator inflammation that triggers several CNS disorders, including neurodegeneration. Tumor necrosis factor-α (TNF-α) ability to stimulate production different inflammatory factors, conditions. Numerous phytochemicals are hypothesized mitigate inflammation, CNS. Among them, flavonoid compound, sophoraflavanone...
Oxidative stress induces miR-200c, the predominant microRNA (miRNA) in lung tissues; however, antioxidant role and biochemistry of such induction have not been clearly defined. Therefore, a adenocarcinoma cell line (A549) normal fibroblast (MRC-5) were used as models to determine effects miR-200c expression on response. Hydrogen peroxide (H2O2) upregulated whose overexpression exacerbated decrease proliferation, retarded progression cells G2/M-phase, increased oxidative upon H2O2...
Naturally evolved immune-escape PreS2 mutant is an oncogenic caveat of liver cirrhosis and hepatocellular carcinoma (HCC) during chronic hepatitis B virus (HBV) infection. prevalent in above 50% patients with HCC. In addition, intrahepatic expression large surface antigen (PreS2-LHBS) induces endoplasmic reticulum stress, mitochondria dysfunction, cytokinesis failure, subsequent chromosome hyperploidy. As PreS2-LHBS has no enzymatic activity, the development PreS2-specific inhibitors can be...
Glucose‐6‐phosphate dehydrogenase (G6PD) is a house keeping enzyme and rate limiting in the pentose phosphate shunt to regenerate NADPH. Tumorigenesis often accompanied by increased glycolysis G6PD activity. However, treatment with TGF‐β, commonly used induce epithelial‐mesenchymal transition (EMT), inhibits expression tumor cells (Accession No. GDS3710). Therefore, roles of on tumorigenesis, particularly EMT need be clearly defined. In present study, whether modulate process cancer through...
Abstract Cancer cells reprogram their glucose metabolism from oxidative phosphorylation to aerobic glycolysis. This metabolic transformation is partly based on the activity alterations of a rate limiting enzyme known as pyruvate kinase M2 (PKM2), which responsible for conversion phosphoenolpyruvate (PEP) into pyruvate. Attributed its critical regulatory role. PKM2 recognized pivotal in cancer By reducing PKM2, attain greater fraction glycolytic metabolites macromolecule synthesis needed...
: Frailty in older adults is associated with adverse health outcomes. This study aimed to analyze the frailty status of community-dwelling Taiwan using Kihon Checklist (KCL) and explore associations demographic, physiological, functional factors.
Purpose Strategy training is a rehabilitation intervention that aims to enhance problem-solving skills with respect daily activity–related challenges and has achieved favorable results in Western countries. This study explored the perspectives of individuals acquired brain injury (ABI) Taiwan who received strategy training.Materials methods Semi-structured interviews community-dwelling adults ABI were conducted, reflective memos made by research team members recorded. Interviews analyzed...
Deficiency of dead cell clearance plays a significant contributing factor in the progression Systemic Lupus Erythematosus (SLE). While several studies support macrophage‐mediated innate immune response for SLE development, mechanism about “silent” by polarized monocyte/macrophage populations still needs to be elucidated. By analyzing miRNA level, expression miR‐210‐5p from peripheral blood mononuclear cells (PBMCs) patients with active (n=17) was higher than that healthy controls (n=16). We...