Osteosarcoma is the most common primary malignant bone tumour, predominantly affecting children and adolescents. Cancer cell line models are required to understand underlying mechanisms of tumour progression for preclinical investigations. To identify lines that well suited studies critical cancer-related phenotypes, such as initiation, growth metastasis, we have evaluated 22 osteosarcoma in vivo tumorigenicity, vitro colony-forming ability, invasive/migratory potential proliferation...

Abstract We have characterized several stages of normal human B cell development in adult bone marrow by gene expression profiling hemopoietic stem cells, early (E-B), pro-B, pre-B, and immature using RNA amplification Lymphochip cDNA microarrays (n = 6). Hierarchical clustering 758 differentially expressed genes clearly separated the five populations. used sets to investigate functional assignment genes. Genes involved VDJ recombination as well lineage-associated transcription factors (TCF3...

Lung cancer (LC) with its different subtypes is generally known as a therapy resistant the highest morbidity rate worldwide. Therapy resistance of tumor thought to be related stem cells (CSCs) within tumors. There have been indications that lung propagated and maintained by small population CSCs. To study this question we established panel 15 primary cell lines (PLCCLs) from 20 fresh tumors using robust serum-free culture system. We subsequently focused on identification CSCs studying these...

// Anastassia Serguienko 1,2 , Iwona Grad Anna B. Wennerstrøm Leonardo A. Meza-Zepeda 1,3 Bernd Thiede 4,5 Eva W. Stratford Ola Myklebost 1,2,5 and Else Munthe 1 Department of Tumor Biology, Institute Cancer Research, The Norwegian Radium Hospital, Oslo University Oslo, Norway 2 Stem Cell Innovation Centre, 3 Genomics Core Facility, 4 Biotechnology Centre 5 Biosciences, Correspondence: Munthe, email: Keywords : ROS, OXPHOS, glycolysis, mitochondria, HMOX1 Received September 17, 2014 Accepted...

Abstract Molecular analysis of circulating tumor DNA (ctDNA) has a large potential for clinical application by capturing tumor-specific aberrations through noninvasive sampling. In gastrointestinal stromal (GIST), KIT and PDGFRA mutations is important therapeutic decisions, but the invasiveness traditional biopsies limits possibilities repeated Using targeted next-generation sequencing, we have analyzed cell-free from 50 GIST patients. Tumor-specific were detected in 16 44 plasma samples...

Wnt/β-catenin is a major regulator of stem cell self-renewal and differentiation this signaling pathway aberrantly activated in several cancers, including osteosarcoma (OS). Attenuation activity by tankyrase inhibitors an appealing strategy treatment OS. The efficacy the inhibitor JW74 was evaluated three OS lines (KPD, U2OS, SaOS-2) both at molecular functional level. At level, induces stabilization AXIN2, key component β-catenin destruction complex, resulting reduced levels nuclear...

The microRNAs in the miR-34 family, consisting of miR-34a, miR-34b and miR-34c, are tumour suppressors. annotated human miR-34b-5p has one additional base at 5' end common family seed sequence, compared to miR-34a-5p miR-34c-5p. This extra results a shift which would affect target gene repertoire have functional consequences. During our studies functions, we investigated precise sequence mature cells by deep sequencing. We found that without was predominant form both non-malignant malignant...

Abstract Despite advances in diagnostics, less than 5% of patients with periampullary tumors experience an overall survival five years or more. Periampullary are neoplasms that arise the vicinity ampulla Vater, enlargement liver and pancreas ducts where they join enter small intestine. In this study, we analyzed copy number aberrations using Affymetrix SNP 6.0 arrays 60 adenocarcinomas from Oslo University Hospital to identify genome-wide aberrations, putative driver genes, deregulated...

Adapter proteins link catalytic signaling to cell surface receptors or downstream effector proteins. In this paper, we present the cDNA sequence F2771, isolated from an activated CD8+ T library. The F2771 encodes a novel putative adapter protein. predicted amino acid includes SH2 domain as well SH3 and phosphotyrosine binding interaction motifs, but lacks any known domains. expression of gene is limited tissues immune system and, in particular, cells. protein expressed by transfected COS...

Liposarcoma cell lines represent in vitro models for studying disease mechanisms at the cellular level and preclinical evaluation of novel drugs. To date there are a limited number well-characterized available. In this study, nine immortal liposarcoma were evaluated tumor-forming ability, stem cell- differentiation potential, metastatic with aim to generate line panel. Detailed marker analyses also performed. Five tumorigenic, forming tumors mice. Interestingly, ability correlated high...

Abstract Background Osteosarcoma is the most common primary malignant tumour of bone occurring in children and young adolescents characterised by complex genetic epigenetic changes. The miRNA miR-486-5p has been shown to be downregulated osteosarcoma cancer general. Results To investigate if mir-486 locus epigenetically regulated, we integrated DNA methylation expression data using cohorts cell lines patient samples. A CpG island promoter ANK1 host gene was highly methylated as determined...

Sarcomas are rare cancers with limited treatment options. Patients generally treated by chemotherapy and/or radiotherapy in combination surgery, and would benefit from new personalized approaches. In this study we demonstrate the potential of combining personal genomic characterization patient tumors to identify targetable mutations vitro testing specific drugs patient-derived cell lines. We have analyzed three metastases a high-grade metastatic dedifferentiated liposarcoma (DDLPS) exome...

No ligand has hitherto been designated for the Eph receptor tyrosine kinase family member, EphB6. Here, expression of an EphB6 in pro‐B leukemic cell line, Reh, is demonstrated by binding soluble EphB6‐Fc fusion protein to Reh cells. The belongs subgroup membrane spanning ligands, as suggested fact that phosphatidylinositol‐specific phospholipase C treatment did not abrogate EphB6‐Fc. Two transmembrane ephrin‐B1 and ephrin‐B2, were identified Analysis or ephrin‐B2 transfected COS cells...

Aldehyde dehydrogenase (ALDH) has recently been shown to be a marker of cancer stem-like cells (CSCs) across tumour types. The primary goals this study were investigate whether ALDH is expressed in liposarcomas, and CSCs can identified the ALDHhigh subpopulation. We have demonstrated that indeed 10 out liposarcoma patient samples. Using xenograft model, we small population with an inducible stem cell potential, expressing both CD133 following culturing medium. This potential CSC population,...

Pancreatic adenocarcinoma is one of the most lethal cancer types, currently lacking efficient treatment. The heterogeneous nature these tumours are poorly represented by classical pancreatic cell lines, which have been through strong clonal selection in vitro, and often derived from metastases. Here, we describe establishment novel models, xenografts corresponding vitro primary tumours. morphology, differentiation grade gene expression pattern resemble original well. lines were analysed for...

The BRAFV600E mutation, which in melanoma is targetable with vemurafenib, also found sarcomas and we here evaluate the therapeutic potential sarcoma cell lines. Methods: Four lines harboring representing liposarcomas (SA-4 SW872), Ewing (A673) atypical synovial (SW982), were treated vemurafenib effects on growth, apoptosis, cycle progression signaling determined. Results: Vemurafenib induced a strong cytostatic effect SA-4 cells, mainly due to arrest, whereas only moderate levels of...

Background: FGFR inhibition has been proposed as treatment for dedifferentiated liposarcoma (DDLPS) with amplified FRS2, but we previously only demonstrated transient cytostatic effects when treating FRS2-amplified DDLPS cells NVP-BGJ398. Methods: Effects of the more potent inhibitor LY2874455 were investigated in three cell lines by measuring on growth and apoptosis vitro also testing efficacy vivo. Genome, transcriptome protein analyses performed to characterize signaling components...

Most tumour-associated antigens (TAA) are non-mutated self-antigens. The peripheral T cell repertoire is devoid of high-avidity TAA-specific cytotoxic lymphocytes (CTL) due to self-tolerance. As tolerance major histocompatibility complex-restricted, cells may be immunized against TAA presented by a non-self human leucocyte antigen (HLA) molecule and transferred cancer patients expressing that HLA molecule. Obtaining allo-restricted CTL low cross-reactivity has, however, proven difficult....

The bone marrow microenvironment regulates early B lymphopoiesis and protects leukemia cells against chemotherapy treatment, thus the may serve as a sanctuary site for these cells. Yet, few factors that contribute to this process are known. We have explored role of transforming growth factor beta (TGFbeta) morphogenetic protein-6 (BMP-6) one target gene, TGFbeta inducible gene 1 (TIEG1), in communication between stroma acute lymphoblastic (ALL) cell lines their escape from chemotherapy....

The Eph receptors are the largest receptor tyrosine kinase family. Several family members expressed in hematopoietic cells. Previously, expression of a member this family, EphA2, was identified on dendritic like cells tonsils. We therefore specifically examined EphA2 vitro generated In study, Langerhans compared to show that ligand induced engagement leads autophosphorylation indicating functional signaling pathway these also observe phosphorylation and dephosphorylation distinct proteins...

Ephrins are ligands for the Eph receptor tyrosine kinases, which play important roles in patterning nervous and vascular systems. Ephrin-A1 is a glycosylphosphatidylinositol-anchored ligand that binds to EphA kinases. In present study, we have identified new ephrin-A1 isoform, denoted ephrin-A1b (ephrin-A1 isoform b). Compared with originally described sequence, ephrin-A1a [Holzman, Marks Dixit (1990) Mol. Cell. Biol. 10, 5830–5838], lacks segment of 22 amino acids (residues 131–152). At...