A5009020376- Microbial Metabolic Engineering and Bioproduction
- Protein Structure and Dynamics
- Gene Regulatory Network Analysis
- Advanced NMR Techniques and Applications
- Salmonella and Campylobacter epidemiology
- Escherichia coli research studies
- Mass Spectrometry Techniques and Applications
- Bacterial Genetics and Biotechnology
- Heat shock proteins research
- Metabolomics and Mass Spectrometry Studies
- NMR spectroscopy and applications
- Enzyme Structure and Function
Biotechnology Institute
2023
Institut National des Sciences Appliquées de Toulouse
2018-2021
Centre National de la Recherche Scientifique
2018-2021
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2021
Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés
2017-2020
Université de Toulouse
2018
Escherichia coli excretes acetate upon growth on fermentable sugars, but the regulation of this production remains elusive. Acetate excretion excess glucose is thought to be an irreversible process. However, dynamic 13C-metabolic flux analysis revealed a strong bidirectional exchange between E. and its environment. The Pta-AckA pathway was found central for both directions, while alternative routes (Acs or PoxB) play virtually no role in consumption. Kinetic modelling predicted that...
Abstract Networks of molecular chaperones maintain cellular protein homeostasis by acting at nearly every step in the biogenesis proteins and complexes. Herein, we demonstrate that major chaperone DnaK/HSP70 model bacterium Escherichia coli is critical for proper functioning central metabolism response to carbon nutrition changes, either directly or indirectly via control heat-shock response. We identified sources whose utilization was positively negatively affected DnaK isolated several...
Quantitative information on the carbon isotope content of metabolites is essential for flux analysis. Whereas this in principle present proton NMR spectra through both direct and long-range heteronuclear coupling constants, spectral overlap homonuclear constants hinder its extraction. We demonstrate here how pure shift 2D J-resolved spectroscopy can simultaneously remove couplings separate chemical from patterns. power method cell lysates different bacterial cultures investigate detail...
We have developed a robust workflow to measure high-resolution fluxotypes (metabolic flux phenotypes) for large strain libraries under fully controlled growth conditions. This was achieved by optimizing and automating the whole high-throughput fluxomics process integrating all relevant software tools. allowed us obtain highly detailed maps of carbon fluxes in central metabolism automated manner. It applied investigate glucose 180 Escherichia coli strains deleted y-genes. Since products these...
Nuclear magnetic resonance (NMR)-based fluxomics seeks to measure the incorporation of isotope labels in selected metabolites follow kinetically synthesis latter. It can however equally be used understand biosynthetic origin same metabolites. We investigate here different NMR approaches optimize such experiments terms resolution and time requirement. Using isoleucine biosynthesis as an example, we explore use field strengths ranging from 500 MHz 1.1 GHz. Because dependence chemical shift...