A5001133114- Metabolomics and Mass Spectrometry Studies
- Microbial Metabolic Engineering and Bioproduction
- Epigenetics and DNA Methylation
- Cancer, Hypoxia, and Metabolism
- Metabolism and Genetic Disorders
- Histone Deacetylase Inhibitors Research
- Sarcoma Diagnosis and Treatment
- Acute Myeloid Leukemia Research
- Isotope Analysis in Ecology
- Clinical Laboratory Practices and Quality Control
- Cancer-related Molecular Pathways
- Pancreatic function and diabetes
- Hormonal and reproductive studies
- 3D Printing in Biomedical Research
- Hemodynamic Monitoring and Therapy
- Probiotics and Fermented Foods
- Mitochondrial Function and Pathology
- Nitrogen and Sulfur Effects on Brassica
- Gut microbiota and health
- Mass Spectrometry Techniques and Applications
- Scientific Measurement and Uncertainty Evaluation
- Viral-associated cancers and disorders
- Salmonella and Campylobacter epidemiology
- Bacterial Genetics and Biotechnology
- Gene Regulatory Network Analysis
Université de Toulouse
2016-2024
Institut National des Sciences Appliquées de Toulouse
2011-2024
Centre National de la Recherche Scientifique
2011-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2024
Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés
2016-2019
Université Toulouse III - Paul Sabatier
2016
Institut National Polytechnique de Toulouse
2016
Centre de recherche Translationnelle en Médecine moléculaire
2013
Laboratoire National de Métrologie et d'Essais
2012
Mass spectrometry (MS) is widely used for isotopic studies of metabolism and other (bio)chemical processes. Quantitative applications in systems synthetic biology require to correct the raw MS data contribution naturally occurring isotopes. Several tools are available low-resolution data, recent developments made substantial improvements by introducing resolution-dependent correction methods, hence opening way high-resolution (HRMS) data. Nevertheless, current HRMS methods partly fail...
Administration of the probiotic Escherichia coli strain Nissle 1917 (EcN) decreases visceral pain associated with irritable bowel syndrome. Mutation clbA, a gene involved in biosynthesis secondary metabolites, including colibactin, was previously shown to abrogate EcN activity. Here, we show that EcN, but not an isogenic clbA mutant, produces analgesic lipopeptide. We characterize lipoamino acids and lipopeptides produced by mutant online liquid chromatography mass spectrometry. One these...
Stable-isotope labeling experiments (ILEs) are widely used to investigate the topology and operation of metabolic networks. The quality isotopic data collected in ILEs is utmost importance ensure reliable biological interpretations, but current evaluation approaches limited due a lack suitable reference material relevant criteria. In this work, we present complete methodology evaluate mass spectrometry (MS) methods for quantitative studies systems. This methodology, based on sample...
Chromatin-bound MDM2 regulates serine metabolism and nucleotide synthesis to sustain tumor growth in liposarcoma.
Significance Pyruvate dehydrogenase (PDH) deficiency is the cause of several human metabolic diseases. In mammals, transcriptional control PDH complex components and its impact on pathophysiology remain poorly understood. We show that E4 transcription factor 1 (E4F1) controls a program essential for activity involves genes linked to syndromes. Genetic inactivation murine E4f1 results in strong decrease severe perturbations pyruvate metabolism. concordance with work Legati et al., we striated...
NMR analysis of the isotope incorporation in amino acids can be used to derive information about topology and operation cellular metabolism. Although traditionally performed by 1H and/or 13C NMR, we present here novel experiments that exploit 15N nucleus same with increased efficiency. Combined a Hα-13CO experiment, increase coverage isotopic space probed obtaining complete distribution species for first two carbons biomass hydrolysates. Our approach was evaluated using as reference material...
Nitrogen remobilization processes from source to sink tissues in plants are determinant for seed yield and their implementation results a complete reorganization of the primary metabolism during sink/source transition. Here, we decided characterize impact balance on amino acid leaves winter oilseed rape grown at vegetative stage. We combined quantitative metabolomics approach with an instationary 15N-labeling experiment by using [15N]L-glycine as metabolic probe leaf ranks gradual increase...
Dedifferentiated and Well-differentiated liposarcoma are characterized by a systematic amplification of the Murine Double Minute 2 (MDM2) oncogene. We demonstrate that p53-independent metabolic functions chromatin-bound MDM2 exacerbated in mediate an addiction to serine metabolism sustain tumor growth. However, origin exogenous remains unclear. Here, we show elevated levels mice harboring liposarcoma-patient derived xenograft, released distant muscle is essential for cell survival....
We have developed a robust workflow to measure high-resolution fluxotypes (metabolic flux phenotypes) for large strain libraries under fully controlled growth conditions. This was achieved by optimizing and automating the whole high-throughput fluxomics process integrating all relevant software tools. allowed us obtain highly detailed maps of carbon fluxes in central metabolism automated manner. It applied investigate glucose 180 Escherichia coli strains deleted y-genes. Since products these...
Studies of the topology, functioning, and regulation metabolic systems are based on two main types information that can be measured by mass spectrometry: (absolute or relative) concentration metabolites their isotope incorporation in 13C-labeling experiments. These data currently obtained from independent experiments because 13C-labeled internal standard (IS) used to determine a given metabolite overlaps 13C-mass fractions which its 13C-isotopologue distribution (CID) is quantified. Here, we...
Cholesterol is one of the most frequently measured substances in human blood/serum to assist assessing health status individuals. Because its clinical significance, CCQM-K6 determination cholesterol serum was completed 2000 as first key comparison (KC) studies performed within Organic Analysis Working Group (OAWG). The subsequent KC for cholesterol, CCQM-K6.1, 2001. Measurements this second subsequent, CCQM-K6.2, were 2012. These comparisons conducted enable CCQM members that had not...
Isocitrate dehydrogenases (IDH) are involved in redox control and central metabolism. We hypothesized that key metabolic fluxes selectively reprogrammed to maintain biosynthetic homeostasis lower drug responses IDH mutant acute myeloid leukemia cells. Here we show reprogramming initiated by IDH1 mutation leads marked increases glucose, glutamine fatty acid catabolism along with enhancement of wild-type enzyme activity contribute provision α-KG required for 2-HG synthesis replenish Krebs...
Abstract Background Metabolic adaptation is a hallmark of cancer including acute myeloid leukemia (AML). Tumor microenvironment also described as an essential support leukemic metabolism. We explored how systemic and tissue metabolism was rewired in leukemia-bearing mice upon chemotherapy. Methods Using AML cell line- primary patient-derived xenograft models, we developed vivo metabolomics to uncover the metabolic pattern 10 tissues plasma, bone marrow, spleen, liver, adipose tissue, lung,...