Fabrication of polycaprolactone (PCL)-chitosan (CS) three-dimensional (3D) scaffolds using the novel technique melt stretching and multilayer deposition was introduced. In brief, firstly, PCL-CS monofilaments containing 0% (pure PCL), 10%, 20% 30% CS by weight were fabricated melting processes. Secondly, desired arranging depositing filaments. Physical properties filaments evaluated. MC3T3-E1 cell lines seeded on to assess their proliferation. A typical micro-groove pattern found surfaces...

Abstract Aim To investigate the biomechanical properties of poly ε‐caprolactone ( PCL )–chitosan CS ) scaffolds fabricated by melt stretching and multilayer deposition technique. Methods The – containing at 0% (pure ), 10%, 20% weight were prepared. For monolayer scaffolds, shear blending tests simulating reconstruction orbital floor defects (situation A mandibular B conducted. 3‐ D compression their superior lateral aspects done. Results pure group had remarkably lower strength than other...

Background:Biocompatibility and degradation of poly ε-caprolactone (PCL)-Biphasic Calcium Phosphate (BCP) scaffolds fabricated by the "Melt Stretching Compression Molding (MSCM)" technique were evaluated in rat models. Objectives:Degradation behaviors histological biocompatibility PC L-20% BCP MSCM compare with those PCL-20% β-tricalcium phosphate (TCP) commercially Fused Deposition Modeling (FDM) evaluated. Methods:The study groups included Group A: B: TCP FDM scaffolds, which implanted...

Physical properties and biocompatibility of polycaprolactone (PCL)–biphasic calcium phosphate (BCP) scaffolds fabricated by the modified melt stretching multilayer deposition (mMSMD) technique were evaluated in vitro. The PCL–BCP scaffold specimens included group A; PCL: BCP (wt%) = 80:20 B; 70:30. Mechanical assessed using a universal testing machine. Degradation behaviors over 60 days. amount ions released from was detected 30 Attachment growth osteoblasts on indirect cytocompatibility to...

Scaffolds of polycaprolactone-30% hydroxyapatite (PCL-30% HA) were fabricated using melt stretching and multilayer deposition (MSMD), the in vitro response osteoblasts to scaffolds was assessed. In group A, immersed 10 µg/mL bone morphogenetic protein-2 (BMP-2) solution prior being seeded with osteoblasts, they cultured medium without BMP-2. B, cell-scaffold constructs BMP-2 containing The results showed greater cell proliferation A. upregulation runt-related transcription factor 2...

Background:Polycaprolactone (PCL)-biphasic calcium phosphate (BCP) scaffolds fabricated using Melt-Stretching and Compression Molding (MSCM) can release ions, which are essential for bone formation. Objective:Responses of the osteoblasts seeded on three groups inc luding group A; PCL-20% BCP (%wt), B; PCL-30% C (control); pure PCL (100% PCL) were evaluated. Methods:The cell-scaffold constructs made by seeding osteoblast cell lines at 1×105 cells/scaffold. The each divided culturing in...

Bioresorbable polymeric membranes for guided bone regeneration (GBR) were fabricated using the three-dimensional printing technique. Membranes made of polylactic-co-glycolic acid (PLGA), which consist lactic (LA) and glycolic in ratios 10:90 (group A) 70:30 B), compared. Their physical characteristics including architecture, surface wettability, mechanical properties, degradability compared vitro, their biocompatibilities vitro vivo. The results demonstrated that group B had strength could...

The ability to repair bone defects of polycaprolactone–chitosan scaffolds containing 20% chitosan (PCL-20%CS) fabricated using the melt stretching and multilayer deposition (MSMD) technique was assessed compared with commercial scaffolds. Two cal

The efficacy of a three-dimensional printed polycaprolactone-biphasic-calcium-phosphate scaffold (PCL-BCP TDP scaffold) seeded with adipose-derived stem cells (ADSCs), which were cultured in xenogeneic serum-free media (XSFM) to enhance bone formation, was assessed vitro and animal models. ADSCs isolated from the buccal fat tissue six patients using enzymatic digestion plastic adherence method. proliferation osteogenic differentiation XSFM when on scaffolds compared those medium containing...

Abstract Alveolar ridge loss presents difficulties for implant placement and stability. To address this, alveolar preservation (ARP) is required to maintain bone avoid the need augmentation using socket grafting. In this study, a scaffold ARP was created by fabricating 3D porous dense microfiber silk fibroin (mSF) embedded in poly(vinyl alcohol) (PVA), which mimics osteoid template. The research utilized freeze–thawing technique create mimicked incorporating different amounts of mSF into...

The polycaprolactone-30% hydroxyapatite (PCL-30%HA) scaffolds were fabricated using Melt Stretching and Multilayer Deposition (MSMD) technique. Different models of guided bone regeneration evaluated in rat models. Group A, the calvarial defects covered with collagen membranes. B, placed into 200 µL 400 µg/mL BMP solution was slowly dripped onto C, soaked before being placed. D, only Conclusively, C model could enhance new formation better than other

Objective: The characteristics of adipose-derived stem cells (ADSC), isolated from buccal fat pads when cultured in media supplemented, with either autologous human serum (AHS group) or fetal bovine (FBS were compared.Material and Methods: Buccal tissue was harvested six patients, who had undergone surgical removal their maxillary third molars underwent orthognathic surgeries. ADSC the tissue, via an enzymatic digestion method AHS FBS groups (n=6/group). Colony forming units-fibroblast...