Aim The aim of this study was to investigate the influence platelet-rich fibrin (PRF) on early wound healing and preservation alveolar ridge shape following tooth extraction. Methods In clinical trial, 20 symmetrical, premolar extraction sockets using split-mouth design were randomly selected with PRF or blood clot. evaluations healing, contour changes, crestal bone resorption performed in dental casts periapical radiographs (T0, initial; T1, 1 week; T2, 2 weeks; T4, 4 T6, 6 T8, 8 weeks)....

Abstract Bone tissue is a highly vascularized tissue. When constructing tissue‐engineered bone models, both the osteogenic and angiogenic capabilities of construct should be carefully considered. However, fabricating to promote vascular formation generation, while simultaneously establishing nutrition channels facilitate nutrient exchange within constructs, remains significant challenge. Triaxial bioprinting, which not only allows independent encapsulation different cell types forming...

Abstract The biphasic calcium phosphate (BCP) concept was introduced to overcome disadvantages of single phase biomaterials. Different composition ratios BCP bioceramics have been studied, yet controversies regarding the effects ratio on biomaterial behavior still exist. In this study, scaffolds were prepared from nano hydroxyapatite (HA) and β‐tricalcium (β‐TCP) that synthesized via a solid state reaction. Three different pure collagen‐based (%HA/%β‐TCP; 30/70, 40/60, 50/50) produced using...

Congenital abnormalities, trauma, and disease result in significant demands for bone replacement the craniofacial region across body. Tetra-compositions of organic inorganic scaffolds could provide advantages regeneration. This research aimed to fabricate characterize amorphous calcium phosphate (ACP)/calcium sulfate hemihydrate (CSH) with alginate/cellulose composite using 3D printing. Alginate/cellulose gels were incorporated 0%, 13%, 15%, 18%, 20%, 23% ACP/CSH one-pot process improve...

Implant placement accuracy in the distal free-end posterior region is often compromised, increasing risk of damage to adjacent anatomical structures and negatively affecting restoration function, occlusal loading, aesthetics. This study aimed assess implant using static computer-assisted surgery (CAIS) partially edentulous area with varying cantilever lengths evaluate correlation between length deviations. A prospective observational involved 40 patients 72 sites, divided into three groups:...

Abstract The aim of this study was to evaluate the accuracy cone-beam computed tomography (CBCT) for determining cortical thickness and gray value, investigating its correlation with micro-computed (CT) histology analysis. Sixty-two bone samples from 4 anatomic regions jaw were analyzed. A radiographic surgical stent used during CBCT sample harvesting. value planned implant evaluated by CBCT. Bone volumetric fractions, mineral density, % porosity assessed micro-CT mineralized material...

Fabrication of polycaprolactone (PCL)-chitosan (CS) three-dimensional (3D) scaffolds using the novel technique melt stretching and multilayer deposition was introduced. In brief, firstly, PCL-CS monofilaments containing 0% (pure PCL), 10%, 20% 30% CS by weight were fabricated melting processes. Secondly, desired arranging depositing filaments. Physical properties filaments evaluated. MC3T3-E1 cell lines seeded on to assess their proliferation. A typical micro-groove pattern found surfaces...

Abstract This study aimed to evaluate in vitro biocompatibility of a composite nanoscale biphasic calcium phosphate (BCP) and collagen (C) compared pure BCP (P) different composition ratios nanohydroxyapatite nano‐β‐tricalcium (HA/β‐TCP). Each group comprised three (30/70, 40/60, 50/50). For evaluation cellular response toward each ratio, mouse osteoblast (MC3T3‐E1) cell line was cultivated on the scaffolds for 19 days. Analysis proliferation, viability, attachment morphology, alkaline...

This study aimed to evaluate the effect of biphasic calcium phosphate (BCP) with high hydroxyapatite/tricalcium (HA/TCP) ratios on bone formation in rabbit calvarial defects.Sixteen New Zealand white rabbits were randomly divided into two groups, a control group and an experimental group. In each animal, bilateral circular defects (10-mm diameter) created calvarium. (three per time frame), grafted autogenous chips one side left empty other side. (five BCP1 (HA:TCP, 8:2) BCP2 9:1)...

Abstract Aim To investigate the biomechanical properties of poly ε‐caprolactone ( PCL )–chitosan CS ) scaffolds fabricated by melt stretching and multilayer deposition technique. Methods The – containing at 0% (pure ), 10%, 20% weight were prepared. For monolayer scaffolds, shear blending tests simulating reconstruction orbital floor defects (situation A mandibular B conducted. 3‐ D compression their superior lateral aspects done. Results pure group had remarkably lower strength than other...

Abstract Bioprinting has demonstrated great advantages in tissue and organ regeneration. However, constructing large-scale organs vitro is still a huge challenge due to the lack of some strategies for loading multiple types cells precisely while maintaining nutrient channels. Here, new 3D bioprinting strategy was proposed construct vascularized tissue. A mixture gelatin methacrylate (GelMA) sodium alginate (Alg) used as bioink, serving outer middle layers single filament triaxial printing...

Abstract The facial and long bones have distinct developmental origins, structures, cellular compositions. This study aimed to compare the in vitro responses of human mandible bone osteoblasts low‐magnitude, high‐frequency (LMHF) mechanical vibration terms expression mediators remodeling. Osteoblast‐like cell cultures were prepared from iliac crest mandibular specimens three individuals cultured osteogenic induction media. Induction mature osteoblastic phenotypes was confirmed by analysis...

Although human tooth was developed for using as a bone graft material due to their similarity in chemical composition compared bone, the clinical application limited autologous use. The aims of this study were develop deproteinized demineralized matrix be used substitution. physicochemical characteristic and osteoblastic cell biocompatibility these materials analyzed compared. Human prepared by 0.5 M HCl. Deproteinization performed via three protocols; (a) thermal treatment, (b) NaOH/thermal...

Tooth presents an intriguing option as a bone graft due to its compositional similarity bone. However, the deproteinized human demineralized tooth matrix (dpDTM), developed overcome limited availability of autologous grafts, has suboptimal pore size and surface roughness. This study aimed fabricate surface-modified dpDTM using acid etching collagen coating, followed by in vitro evaluation physicochemical biological properties. The was modified into two protocols: Acid-modified (A-dpDTM)...

Abstract Aim The aim of the present study was to investigate temporal and spatial gene expression bone morphogenetic proteins ( BMP )‐2, ‐4, ‐7, transforming growth factor‐β TGF ‐β), during distraction process rabbit mandible. Methods Twenty rabbits each had an osteotomy on left mandibular body, devices were fixed. After a delay 3 days, started at rate 0.5 mm/12 h for 10 followed by 3‐week consolidation phase. Four killed 5 days distraction, 1, 2, weeks after completion distraction....

Abstract Aim: To assess and compare the efficacy of single buccal palatal infiltration lidocaine, mepivacaine, or articaine with 1:100 000 epinephrine by maxillary anesthetic injection. Methods: A double‐blinded, randomized, clinical trial was conducted 33 patients undergoing upper premolar extraction. The were randomly allocated into one three groups, according to local agent used: 2% 4% articaine, all epinephrine, blinded used. extent anesthetization, pulpal anesthetization in adjacent...

Objective: To assess the potential of using autologous demineralized tooth matrix (aDTM) in combination with platelet-rich fibrin (PRF) membrane (aDTM/PRF) or PRF alone (control) to preserve ridge dimension and facilitate bone healing after extraction. Materials Methods: Forty premolar sockets were assigned either aDTM/PRF control group. Horizontal vertical changes evaluated at baseline 2, 4, 6, 8 weeks cast-based periapical radiographs. Results: aDTM was well tolerated all sites without...

The ability to repair bone defects of polycaprolactone–chitosan scaffolds containing 20% chitosan (PCL-20%CS) fabricated using the melt stretching and multilayer deposition (MSMD) technique was assessed compared with commercial scaffolds. Two cal