A5067019820- Parasite Biology and Host Interactions
- Marine Ecology and Invasive Species
- Parasites and Host Interactions
- Identification and Quantification in Food
- Helminth infection and control
- Parasitic infections in humans and animals
- Bird parasitology and diseases
- Leech Biology and Applications
- Nematode management and characterization studies
- Ichthyology and Marine Biology
- Fish Biology and Ecology Studies
- Parasitic Infections and Diagnostics
- Turtle Biology and Conservation
- Myxozoan Parasites in Aquatic Species
- Genetic diversity and population structure
- Parasitic Diseases Research and Treatment
- Cephalopods and Marine Biology
- Marine Biology and Ecology Research
- Amoebic Infections and Treatments
- Marine animal studies overview
- Organ Donation and Transplantation
- Marine and fisheries research
- Aquatic Invertebrate Ecology and Behavior
- Isotope Analysis in Ecology
- Aquaculture disease management and microbiota
Sapienza University of Rome
2016-2025
Policlinico Umberto I
2017-2024
Istituto Pasteur
2018-2021
Università degli Studi della Tuscia
2017
Beltsville Agricultural Research Center
2014
Agricultural Research Service
2014
Numerous specimens of the 3 sibling species Anisakis simplex complex (A. pegreffii, A. (senso stricto)), and sp. C) recovered from cetacean stranded within known geographical ranges these nematodes were studied morphologically genetically. The genetic characterization was performed on diagnostic allozymes sequences analysis nuclear (internal transcribed spacer [ITS] ribosomal [r]DNA) mitochondrial (mitochondrial [mt]DNA cox2 rrnS) genes. These markers showed (1) occurrence sympatry 2...
Abstract Surveillance data published since 2010, although limited, showed that there is no evidence of zoonotic parasite infection in market quality Atlantic salmon, marine rainbow trout, gilthead seabream, turbot, meagre, halibut, common carp and European catfish. No studies were found for greater amberjack, brown African catfish, eel pikeperch. Anisakis pegreffii, A. simplex (s. s.) Cryptocotyle lingua seabass, bluefin tuna and/or cod, Pseudamphistomum truncatum Paracoenogonimus ovatus...
Isozyme analysis at 24 loci was carried out on anisakid nematodes of the Anisakis simplex complex, recovered from various intermediate/paratenic (squid, fish) and definitive (marine mammals) hosts parts world. A number samples were found to belong A. sensu stricto pegreffii, widely extending geographic ranges these 2 species. In addition, a new distinct gene pool detected, showing different alleles with respect s. str pegreffii 5 diagnostic (99% level). Samples this assigned species,...
Human cases of gastric anisakiasis caused by the zoonotic parasite Anisakis pegreffii are increasing in Italy. The disease is ingestion larval nematodes lightly cooked or raw seafood. Because symptoms vague and serodiagnosis difficult, often misdiagnosed understimated.
The genetic relationships among 9 taxa of Anisakis Dujardin, 1845 (A. simplex (sensu stricto), A. pegreffii, C., typica, ziphidarum, physeteris, brevispiculata, paggiae, and sp.) were inferred from sequence analysis (629 bp) the mitochondrial cox2 gene. Genetic divergence considered taxa, estimated by p-distance, ranged p = 0.055, between sibling species complex, to 0.12, morphologically differentiated species, i.e., ziphidarum typica. highest level was detected when comparing paggiae versus...
Abstract Anisakiasis is an emerging zoonosis caused by the fish parasitic nematode Anisakis . Spain appears to have highest reported incidence in Europe and marinated anchovies are recognised as main food vehicle. Using data on fishery landings, infection rates consumption habits of Spanish population from questionnaires, we developed a quantitative risk assessment (QRA) model for anchovy value chain. Spaniards were estimated consume average 0.66 per untreated (non-frozen) raw or meal. A...
Anisakiasis is an important fish-borne zoonosis provoked by larval stages of nematodes belonging to the genus Anisakis. The detection and identification human infections difficult. This due to: a) low specificity clinical features symptomatology related infections; b) paucity diagnostic larvae found in granulomatous lesions characteristic "invasive anisakiasis"; c) lack morphological characters at specific level when Anisakis are detected. Thus, molecular-based approaches warranted. We have...
Anisakid nematodes belonging to the species Anisakis pegreffii are distributed in a wide variety of fishes from Mediterranean Sea and they known cause human zoonosis anisakiasis. The present study investigated, for first time, response A. larvae (identified level by allozymes mtDNA cox2 sequence analysis) storage temperature European anchovies, Engraulis encrasicolus. larval motility was studied 1300 fish specimens, captured highly infested area (FAO 37.2.1, 43°8′N, 14°16′E), maintained...
The occurrence of seven species the larval parasitic nematode Anisakis , which can be used as a biological tag for hake Merluccius merluccius stocks throughout their geographical range, is reported. Hake were collected from 14 localities in Mediterranean Sea and Atlantic Ocean. larvae ( n = 1950), recovered, identified to by means genetic markers (allozymes). Within type I, A. pegreffii simplex s.s ., typica ziphidarum detected, while within II, physeteris brevispiculata sp. identified....
SUMMARY A multi-marker nuclear genotyping approach was performed on larval and adult specimens of Anisakis spp. ( N = 689) collected from fish cetaceans in allopatric sympatric areas the two species pegreffii simplex (s. s.), order to: (1) identify belonging to parental taxa by using markers (allozymes loci) sequence analysis a new diagnostic DNA locus (i.e. partial EF1 α −1 n region) (2) recognize hybrid categories. According Bayesian clustering algorithms, based those markers, most...
In order to describe the molecular epidemiology of Blastocystis infection in Italy, 189 isolates, which had been collected during years 2012-2014 from mildly symptomatic patients, or those affected by inflammatory bowel disease (IBD), irritable syndrome (IBS) chronic diarrhoea, otherwise immunosuppressed, were subtyped sequence analysis SSU rRNA gene (536 bp). Six subtypes (STs) detected: ST1 (15·3%), ST2 (13·8%), ST3 (46·0%), ST4 (21·7%), ST6 (3·2%) and ST8 (0·5%). They clustered distinct...
Abstract Parasite biodiversity in cetaceans represents a neglected component of the marine ecosystem. This study aimed to investigate distribution and genetic diversity anisakid nematodes genus Anisakis sampled from Northeast Atlantic Ocean Mediterranean Sea. A total 478 adults pre-adults spp. was identified by multilocus approach (mtDNA cox 2 , EF1 α − 1 nDNA nas 10 gene loci) 11 cetacean species. clear pattern host preference observed for at family level: A. simplex (s.s.) pegreffii...