A5076097159- Cardiac Ischemia and Reperfusion
- Immune Response and Inflammation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Gastrointestinal motility and disorders
- Renal function and acid-base balance
- Diet and metabolism studies
- Nitric Oxide and Endothelin Effects
- Helicobacter pylori-related gastroenterology studies
- Clinical Nutrition and Gastroenterology
- Cell Adhesion Molecules Research
- Respiratory Support and Mechanisms
- Gastroesophageal reflux and treatments
- Liver Disease and Transplantation
- Sepsis Diagnosis and Treatment
- Neuropeptides and Animal Physiology
- Dietary Effects on Health
- Hemodynamic Monitoring and Therapy
- Immune cells in cancer
- Organ Transplantation Techniques and Outcomes
- Inhalation and Respiratory Drug Delivery
- Electrolyte and hormonal disorders
- Anesthesia and Sedative Agents
- Blood Coagulation and Thrombosis Mechanisms
- Liver Disease Diagnosis and Treatment
- Hemoglobin structure and function
Alfaisal University
2014-2023
King Faisal Specialist Hospital & Research Centre
2015-2019
Lawson Health Research Institute
2001-2010
Western University
1998-2009
London Health Sciences Centre
1962-2008
Center for Vascular Biology Research
2001
Victoria Hospital
1995-1997
Kyoto Prefectural University of Medicine
1997
University Medical Center
1987-1995
University Hospital and Clinics
1987-1995
Helicobacter pylori-associated gastritis is mainly an inflammatory cell response. In earlier work we showed that activation of human neutrophils by a cell-free water extract H. pylori characterized increased expression neutrophil CD11b/CD18 and adhesiveness to endothelial cells. The reported here indicates the neutrophil-activating factor 150,000-molecular-weight protein (150K protein). Neutrophil proadhesive activity copurified with this protein, which polymer identical 15K subunits....
The aim of the present study was to characterize and compare expression intercellular adhesion molecule 1 (ICAM-1) on unstimulated endotoxin-challenged endothelial cells in different tissues rat. ICAM-1 measured using 125I-labeled anti-rat monoclonal antibody (MAb) an isotype-matched control MAb labeled with 131I (to correct for nonspecific accumulation binding MAb). Under baseline conditions, observed all organs. 125I-ICAM-1 varied widely among organs, largest (per g tissue) lung, followed...
The objective of this study was to determine whether superoxide mediates the leukocyte-endothelial cell interactions elicited by reperfusion (reoxygenation) ischemic (hypoxic) tissues. Mesenteric and intestinal blood flows were reduced 20% control for 1 h, followed h reperfusion. Sixty minutes after reperfusion, red velocity (Vr), leukocyte rolling (Vw), number adherent leukocytes measured in mesenteric venules. Then, either human dismutase (hSOD), hydrogen peroxide-inactivated hSOD, or MoAb...
Previous studies have shown enhanced neutrophil adhesion to endothelial cells exposed anoxia and then reoxygenated (A/R). To define the molecular basis for these observations, we evaluated relative roles of CD11/CD18 determinants (CD11a CD11b) neurtrophils proteins intercellular molecule 1 (ICAM-1) endothelial-leukocyte (ELAM-1). Human umbilical vein cell (HUVEC) monolayers were 30 min, reoxygenated, reacted with 51Cr-labeled neutrophils in assays. Neutrophil HUVEC A/R was significantly...
The role of neural, metabolic, physical, and humoral factors in the intestinal hyperemia associated with chronic portal hypertension was examined by use rat vein stenosis model. Intestinal blood flow splenic pulp pressure were increased, while systemic arterial total vascular resistance reduced vein-stenosed rats as compared controls. reduction entirely due to a fall precapillary accompanied an increase capillary pressure, which exceeded that produced acute elevation same level....
The objectives of this study were to (1) determine the time course neutrophil adhesion monolayers human umbilical vein endothelial cells (HUVECs) that exposed 60 minutes anoxia followed by 30 600 reoxygenation and (2) define mechanisms responsible for both early (minutes) late (hours) hyperadhesivity postanoxic HUVECs neutrophils. results clearly demonstrate anoxia/reoxygenation (A/R) leads a biphasic increase in HUVECs, with peak responses occurring at (phase 1) 240 2) after reoxygenation....
In autoperfused preparations of feline jejunum, blood flow was measured from the mucosal surface with laser-Doppler velocimetry (LDV) and hydrogen gas (H2) clearance techniques while altered by intra-arterial infusions isoproterenol. LDV H2 estimates were compared total-wall mucosal-submucosal flows radiolabeled microsphere technique. Over range (26.3–73.6 ml X min-1 100 g-1) attained, a series direct linear relationships obtained among LDV, clearance, jejunal flow. The slopes these...
Objective: Erythropoietin (EPO) prevents the myocardial dysfunction induced by ischemia/reperfusion (I/R). Since I/R-induced is associated with an acute inflammatory response, we assessed anti-inflammatory properties of EPO using in vitro and vivo models I/R.
ABSTRACT One of the cardinal signs acute inflammation is neutrophil (PMN) emigration across endothelium and into affected tissue. We have previously shown that human PMN migration umbilical vein endothelial cell (HUVEC) monolayers dependent on PMN-derived elastase. However, whether migrating release elastase extracellular milieu or retain it surface unclear. In present study, we show when are activated by platelet activating factor (PAF), was mobilized to retained in membrane; no activity...
Blood flows to the major organs of resting conscious dog were measured prior and 30 90 min after feeding using radioactive microsphere technique. Mean systemic arterial pressure, heart rate, PO2, PCO2, pH, as well blood flow brain, heart, adrenals, skeletal muscle, hepatic artery, gastric antrum not significantly changed following meal. Pancreatic duodenal jejunal increased at both min, whereas ileal only feeding. Flow body in half fed animals, but it all animals treated with histamine. In...
Abstract High mobility group box 1 (HMGB1) is an alarmin actively secreted by immune cells and passively released necrotic nonimmune cells. HMGB1 has been implicated in both cardiac contractile dysfunction the lethality associated with sepsis/endotoxemia. The aim of current study was to assess whether viable cardiomyocytes could produce can affect myocardial contractility. LPS used as a model sepsis/endotoxemia mice isolated myocytes. increased expression vivo (immunohistochemistry)...