A5027955731- Mesenchymal stem cell research
- Corneal Surgery and Treatments
- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- Tissue Engineering and Regenerative Medicine
- Cancer Cells and Metastasis
- Glycosylation and Glycoproteins Research
- Immune Cell Function and Interaction
- Extracellular vesicles in disease
- Cancer Research and Treatments
- Immune cells in cancer
- Ocular Surface and Contact Lens
- Galectins and Cancer Biology
- Corneal surgery and disorders
- T-cell and B-cell Immunology
- Osteoarthritis Treatment and Mechanisms
- Immune Response and Inflammation
- Colorectal Cancer Treatments and Studies
- Wound Healing and Treatments
- Virus-based gene therapy research
- Neonatal Respiratory Health Research
- RNA Interference and Gene Delivery
- Pluripotent Stem Cells Research
- Xenotransplantation and immune response
- Traumatic Brain Injury and Neurovascular Disturbances
Ollscoil na Gaillimhe – University of Galway
2016-2025
Regenerative Medicine Institute
2021
Queensland Health
2017-2018
University Hospital Galway
2016
Mesenchymal stem cells (MSCs) inhibit proliferation of allogeneic T and express low levels major histocompatibility complex class I (MHCI), MHCII vascular adhesion molecule-1 (VCAM-1). We investigated whether their immunosuppressive properties immunophenotype protect rat MSCs against cytotoxic lysis in vitro result a reduced immune response vivo. Rat were partially protected alloantigen-specific vitro. However, after treatment with IFN-γ IL-1β, upregulated MHCI, VCAM-1, was significantly...
Immunosuppressive tumor microenvironments (TMEs) reduce the effectiveness of immune responses in cancer. Mesenchymal stromal cells (MSCs), precursors to cancer-associated fibroblasts (CAFs), promote progression by enhancing cell suppression colorectal cancer (CRC). Hyper-sialylation glycans promotes evasion through binding sialic acids their receptors, Siglecs, expressed on cells, which results inhibition effector functions. The role sialylation shaping MSC/CAF immunosuppression TME is not...
Allogeneic mesenchymal stem cells (allo-MSCs) have potent regenerative and immunosuppressive potential are being investigated as a therapy for osteoarthritis; however, little is known about the immunological changes that occur in allo-MSCs after ex vivo induced or differentiation. Three-dimensional chondrogenic differentiation was an alginate matrix, which served to immobilize potentially protect MSCs at site of implantation. We show allogeneic differentiated lost ability inhibit T-cell...
Abstract Stromal cells of mesenchymal origin reside below the epithelial compartment and provide structural support in intestine. These intestinal stromal interact with both cell compartments, as well infiltrating hematopoietic immune cells. The importance these regulating homeostasis during inflammation is recognized. However, little known about their function phenotype inflammatory tumor microenvironment. Using a syngeneic, immunogenic model colorectal cancer, we showed that TNFα-initiated...
Mesenchymal stromal cells (MSCs) are a promising therapeutic option for multiple immune diseases/disorders; however, efficacy of MSC treatments can vary significantly. We present novel licensing strategy to improve the immunosuppressive capacity MSCs. Licensing murine MSCs with transforming growth factor-β1 (TGF-β MSCs) significantly improved their ability modulate both phenotype and secretome inflammatory bone marrow-derived macrophages increased numbers regulatory T lymphocytes following...
High-risk cornea transplant recipients represent a patient population with significant un-met medical need for more effective therapies to prevent immunological graft rejection due heightened anti-donor immune response. In this study, rat model of pre-existing immunity was developed in which corneal allografts were rejected earlier than non-pre-sensitized recipients. model, third-party (non-donor, non-recipient strain) allogeneic mesenchymal stromal cells (allo-MSC) administered...
Mesenchymal stromal cells (MSCs) have shown promise as a therapy for immune-mediated disorders, including transplant rejection. Our group previously demonstrated the efficacy of pretransplant, systemic administration allogeneic but not syngeneic MSCs in rat cornea model. The aim this study was to enhance immunomodulatory capacity MSCs. In vitro, licensed with TNF-α/IL-1β (MSCsTNF-α/IL-1β) suppress lymphocyte proliferation via NO production. vivo, when administered post-transplantation,...
CMS4 colorectal cancer (CRC), based on the consensus molecular subtype (CMS), stratifies patients with poorest disease-free survival rates. It is characterized by a strong mesenchymal stromal cell (MSC) signature, wound healing-like inflammation and therapy resistance. We utilized 2D 3D
Mesenchymal Stromal Cell derived extracellular vesicles (MSC-EVs) may retain the cancer targeting and immune privilege of MSCs. The immense potential MSC-EVs hold as tumour-targeted therapeutics warrants an understanding adverse events to support clinical translation. This study aimed determine whether would elicit response following administration in tumour-bearing immunocompetent animals. Secreted EVs were isolated from both human murine bone marrow MSCs characterized. hMSC-EVs or mMSC-EVs...
The immunosuppressive tumour microenvironment (TME) reduces immune response effectiveness in stromal-rich tumours, including CMS4 colorectal cancer (CRC). Mesenchymal stromal cells (MSCs), precursors to cancer-associated fibroblasts (CAFs), promote progression by suppressing anti-tumour responses. Hypersialylation of glycans on tumours engages Siglec receptors cells, driving dysfunction, but its role stromal-mediated immunosuppression remains unclear. Sialic acids and ligands were measured...
Adult mesenchymal stem cells (MSCs) are non-hematopoietic with multi-lineage potential which makes them attractive targets for regenerative medicine applications. However, to date, therapeutic success of MSC-therapy is limited and the genetic modification MSCs using viral vectors one option improve their potential. Ex-vivo recombinant adenovirus (Ad) could be promising reduce undesired immune responses as Ad will removed before cell/tissue transplantation. In this regard, we investigated...
Mesenchymal stem/stromal cells (MSC) are an immunomodulatory cell population which under preclinical and clinical investigation for a number of inflammatory conditions including transplantation. In this study, well-established rat corneal transplantation model was used to test the ability human MSC prolong allograft rejection-free survival using pre-transplant intravenous infusion protocol previously shown be efficacious with allogeneic MSC. Surprisingly, administration had no effect on...
(2021). Cyclophosphamide alters the tumor cell secretome to potentiate anti-myeloma activity of daratumumab through augmentation macrophage-mediated antibody dependent cellular phagocytosis. OncoImmunology: Vol. 10, No. 1, 1859263.
Investigations into cell therapies for application in organ transplantation have grown. Here, we describe the ex vivo generation of donor bone marrow-derived dendritic cells (BMDCs) and glucocorticoid-treated BMDCs with potent immunomodulatory properties allogeneic transplantation. were treated dexamethasone (Dexa) to induce an immature, maturation-resistant phenotype. BMDC Dexa phenotype, antigen presenting function, fully characterized. Both populations display significant properties,...
Dendritic cellular therapies and dendritic cell vaccines show promise for the treatment of autoimmune diseases, prolongation graft survival in transplantation educating immune system to fight cancers. Cell surface glycosylation plays a crucial role cell-cell interaction, uptake antigens, migration, homing DCs. Glycosylation is known change with environment functional state Tolerogenic DCs (tDCs) are commonly generated using corticosteroids including dexamethasone, however, date little on how...
Abstract Background Systemic administration of mesenchymal stromal cells (MSCs) has been efficacious in many inflammatory disease settings; however, little data are available on the potential immunomodulatory effects following local MSC context corneal transplantation. The purpose this study was to assess subconjunctival injection MSCs promote allograft survival. Methods were isolated from female C57BL/6 (H-2 k ) or Balb/c d mice and extensively characterized. An allogeneic mouse transplant...