A5089842089- Extracellular vesicles in disease
- Mitochondrial Function and Pathology
- Child and Adolescent Psychosocial and Emotional Development
- Cancer Immunotherapy and Biomarkers
- Genetics, Aging, and Longevity in Model Organisms
- Microtubule and mitosis dynamics
- Redox biology and oxidative stress
- Listeria monocytogenes in Food Safety
- Microbial Inactivation Methods
- Cancer Cells and Metastasis
- MicroRNA in disease regulation
- Hippo pathway signaling and YAP/TAZ
- Youth Substance Use and School Attendance
- Prion Diseases and Protein Misfolding
- Wnt/β-catenin signaling in development and cancer
- Diagnosis and Treatment of Venous Diseases
- Mental Health Treatment and Access
- Machine Learning in Healthcare
- Patient Satisfaction in Healthcare
- Identification and Quantification in Food
- Immune cells in cancer
- Adolescent and Pediatric Healthcare
- Maternal Mental Health During Pregnancy and Postpartum
- Electronic Health Records Systems
- Characterization and Applications of Magnetic Nanoparticles
UNSW Sydney
2023-2025
Black Dog Institute
2023-2025
Ollscoil na Gaillimhe – University of Galway
2015-2025
Southern Health and Social Care Trust
2022
Monash University
2015
University of Leeds
2005
Ageing is associated with mitochondrial dysfunction and increased oxidative stress. Exercise generates endogenous reactive oxygen species (ROS) promotes rapid remodelling. We investigated the role of Peroxiredoxin 2 (PRDX-2) in adaptations to exercise ageing using Caenorhabditis elegans as a model system. PRDX-2 was required for remodelling response mediated by DAF-16 transcription factor activation regulation fusion gene eat-3. Employing an acute recovery cycle, we demonstrated...
The transfer of information and metabolites between mitochondria the ER is mediated by mitochondria-ER contact sites (MERCS), facilitating adaptations following changes in cellular homeostasis. MERCS are dynamic structures, essential for maintaining homeostasis through modulation calcium transfer, redox signalling, lipid autophagy mitochondrial dynamics. Acute stress myoblasts promoted myogenesis that required PERK arm UPRER increased assembly, turnover function. Similarly, induction acute...
Abstract Background Primary school students struggling with mental health are less likely than high to access care, due barriers such as stigma and low literacy among children parents. The near universal reach of schools offers a potential avenue increase care through early identification. risks this approach also need be understood. This study monitored the impact screening for symptoms on outcomes primary students. Methods Across 6 schools, cluster randomised controlled trial allocated one...
Abstract Extracellular vesicles (EVs) are versatile transporters of genetic cargo with enormous potential in the therapeutic setting. Scalable production EVs, and routes to overcome rapid clearance required. Biocompatible hydrogels may support precise, localized delivery EVs target sites. This study aimed establish sustained a scalable 3D dynamic bioreactor fabricate using tyramine-modified hyaluronic acid (HA-TA) EV integration release patterns. MDA-MB-231 cells transduced lentiviral GFP...
Mesenchymal Stromal Cell derived extracellular vesicles (MSC-EVs) may retain the cancer targeting and immune privilege of MSCs. The immense potential MSC-EVs hold as tumour-targeted therapeutics warrants an understanding adverse events to support clinical translation. This study aimed determine whether would elicit response following administration in tumour-bearing immunocompetent animals. Secreted EVs were isolated from both human murine bone marrow MSCs characterized. hMSC-EVs or mMSC-EVs...
Fasciola hepatica, or liver fluke, causes fasciolosis in humans and livestock. Following ingestion of vegetation contaminated with encysted parasites, metacercariae, newly excysted juveniles (NEJ) excyst the small intestine cross intestinal wall. After penetrating liver, parasite begins an intra-parenchymal migratory feeding phase that not only drives their rapid growth development but also extensive haemorrhaging immune pathology. Studies on infection are hindered by difficulty accessing...
Extracellular vesicles (EVs) shuttle microRNA (miRNA) throughout the circulation and are believed to represent a fingerprint of releasing cell. We isolated characterized serum EVs breast tumour-bearing animals, cancer (BC) patients, healthy controls. were using transmission electron microscopy (TEM), protein quantification, western blotting, nanoparticle tracking analysis (NTA). Absolute quantitative (AQ)-PCR was employed analyse EV-miR-451a expression. Isolated had appropriate morphology...
CMS4 colorectal cancer (CRC), based on the consensus molecular subtype (CMS), stratifies patients with poorest disease-free survival rates. It is characterized by a strong mesenchymal stromal cell (MSC) signature, wound healing-like inflammation and therapy resistance. We utilized 2D 3D
Abstract Background Evidence suggests that cognitive bias modification of interpretations (CBM‐I) is effective in modifying interpretation biases and has a small effect on reducing anxiety children adolescents. However, most evidence to date based studies which report or general distress using ad‐hoc Likert‐type Visual Analogue Scales, are useful but do not reliably index symptoms clinical importance. This meta‐analysis aimed establish the effects CBM‐I for adolescents both depression...
Comparing health-related quality of life (HRQL) outcomes between studies is difficult due to the wide variety instruments used. study and facilitating pooled data analyses requires valid "crosswalks" HRQL instruments. Algorithms exist map 12-item Short Form Health Survey (SF-12) responses EQ-5D item preference weights, but none have been validated in populations where disability prevalent, such as injury.Data were extracted from Validating Improving injury Burden Estimates Study...
The alternative sigma factor B (σ
Abstract Plastid ribosomal proteins (PRPs) can play essential roles in plastid ribosome functioning that affect plant function and development. However, the of many PRPs remain unknown, including elucidation which are or display redundancy. Here, we report nuclear-encoded PLASTID RIBOSOMAL PROTEIN L5 (PRPL5) is for early embryo development A. thaliana , as homozygous loss-of-function mutations PRPL5 gene impairs chloroplast leads to failure develop past globular stage. We confirmed prpl5...
Colorectal cancer (CRC) is the third leading cause of cancer-related deaths worldwide. CRC develops in a complex tumour microenvironment (TME) with both mesenchymal stromal cells (MSCs) and immune infiltrate, shown to alter disease progression treatment response. We hypothesised that an accessible, affordable model combines multiple cell types will improve research translation clinic enable identification novel therapeutic targets. A viable gelatine-methacrloyl-based hydrogel culture system...
Centrosome amplification (CA) is a prominent feature of human cancers linked to tumorigenesis in vivo. Here, we report mechanistic contributions CA induction alone tumour architecture and extracellular matrix (ECM) remodelling. non-tumorigenic breast cells MCF10A causes cell migration invasion, with underlying disruption epithelial cell-cell junction integrity dysregulation expression subcellular localisation proteins. also elevates integrin β-3, its binding partner fibronectin-1...
Abstract Ageing is associated with mitochondrial dysfunction and increased oxidative stress. Exercise generates endogenous reactive oxygen species (ROS) promotes rapid remodelling. We investigated the role of Peroxiredoxin 2 (PRDX-2) in adaptations to exercise ageing using Caenorhabditis elegans as a model system. PRDX-2 was required for remodelling response mediated by DAF-16 nuclear localisation. Employing an acute recovery cycle, we demonstrated exercise-induced ER contact sites (MERCS)...
This study investigates the effect of a new short-stretch two-layer compression system in eight patients with common chronic lower-limb conditions three locations UK. Chronic leg ulcers are most type ulceration 70% caused by venous hypertension. An appropriate level is proven to heal ulcers. The was only small sample patients; however, underlying included eczema, diabetes, sarcoma, cellulitis and mixed-aetiology During study, UK experienced some hottest temperatures last 30 years. had an...
Abstract The alternative sigma factor B (σ ) contributes to the stress tolerance of foodborne pathogen Listeria monocytogenes by upregulating General Stress Response. We previously showed that σ loss-of-function mutations arise frequently in strains L. , and suggested mild stresses might favour selection such mutations. In this study, we performed vitro evolution experiments (IVEE) where was allowed evolve over 30 days at elevated (42°C) or lower (30°C) incubation temperatures. Isolates...
ABSTRACT Centrosome amplification (CA) is a prominent feature of human cancers linked to genomic instability and tumourigenesis in vivo . CA observed as early pre-malignant metaplasia, with incidence increasing the disease progresses from dysplasia neoplasia. However, mechanistic contributions (tumour architecture remodelling) are poorly understood. Using non-tumourigenic breast cells (MCF10A), we demonstrate that induction (by CDK1 inhibition or PLK4 overexpression) alone increased both...