A5002860937- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Circular RNAs in diseases
- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- Cancer-related molecular mechanisms research
- Nanoplatforms for cancer theranostics
- Mesenchymal stem cell research
- Advanced biosensing and bioanalysis techniques
- Cell Adhesion Molecules Research
- Nanoparticle-Based Drug Delivery
Harvard University
2020-2023
Massachusetts General Hospital
2020-2023
Harvard NeuroDiscovery Center
2023
Vanderbilt University
2020
Ollscoil na Gaillimhe – University of Galway
2016-2020
Duke University
2020
Extracellular vesicles (EVs) are membrane-encapsulated particles that carry genetically active and protein/lipid cargo can affect the function of recipient cell. A number studies have described effect these on cells demonstrated their promise as therapeutic delivery vectors. Here we demonstrate functional EV-encapsulated RNA protein through use luminescence fluorescence reporters by combining organelle-targeted nanoluciferase with fluorescent proteins. We highlight a mechanism which retain...
Extracellular vesicles (EVs) shuttle microRNA (miRNA) throughout the circulation and are believed to represent a fingerprint of releasing cell. We isolated characterized serum EVs breast tumour-bearing animals, cancer (BC) patients, healthy controls. were using transmission electron microscopy (TEM), protein quantification, western blotting, nanoparticle tracking analysis (NTA). Absolute quantitative (AQ)-PCR was employed analyse EV-miR-451a expression. Isolated had appropriate morphology...
In the central nervous system (CNS), crosstalk between neural cells is mediated by extracellular mechanisms, including brain-derived vesicles (bdEVs). To study endogenous communication across brain and periphery, we explored Cre-mediated DNA recombination to permanently record functional uptake of bdEVs cargo over time. elucidate transfer within at physiological levels, promoted continuous secretion levels containing Cre mRNA from a localized region in situ lentiviral transduction striatum...
Tools to effectively demonstrate and quantify functional delivery in cellular communication have been lacking. This study reports the use of a fluorescently labeled split Nanoluc reporter system transfer between cells vitro subcutaneous tumor mouse model. Our construct allows monitoring direct, indirect, specifically extracellular vesicle-mediated communication.
In glioblastoma, a malignant primary brain tumor, liposomes have shown promise in pre-clinical and early phase clinical trials as delivery vehicles for therapeutics. However, external factors influencing cellular uptake of glioma cells are poorly understood. Heparin heparin analogues commonly used patients to decrease the risk thrombo-embolic events. Our results show that inhibits pegylated liposome by U87 GL261 dose dependent manner vitro, heparin-mediated inhibition required presence fetal...
The ability to edit the human genome treat previously incurable diseases or modify undruggable targets has potential transform lives of patients. However, delivery editing machineries in vivo, and a transient manner, continues be major challenge. We engineered cell-derived ARRDC1-mediated microvesicles (ARMMs) as non-viral vehicles that mediate intracellular proteins, including DNA-modifying enzymes such Cre recombinase Cas9/guide RNA ribonucleoproteins (RNPs). ARMMs packaged multiple...
In the central nervous system (CNS), crosstalk between neural cells is mediated by extracellular mechanisms, including brain-derived vesicles (bdEVs). To study endogenous communication across brain and periphery, we explored Cre-mediated DNA recombination to permanently record functional uptake of bdEVs cargo overtime. elucidate transfer within at physiological levels, promoted continuous secretion levels containing Cre mRNA from a localized region in
Abstract Mesenchymal Stem Cells (MSCs) are multipotent stromal cells known to migrate specifically the sites of tumors and metastases, raising potential as tumor-targeted delivery vehicles for therapeutic agents. MSCs secrete exosomes containing genetic material including microRNAs (miRs), that effectively taken up by cells. Previous work has shown miR-379 be significantly reduced in breast tumor tissue, highlighting a suppressor role. This study aimed establish mechanism action miR-379,...
Extracellular Vesicles and Circulating Nucleic Acids is an open access journal, focusing on extracellular vesicles circulating nucleic acids including DNA, RNA, miRNA their therapeutic use.