A5047418888- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Virus-based gene therapy research
- Extracellular vesicles in disease
- Antimicrobial Peptides and Activities
- Lipid Membrane Structure and Behavior
- Monoclonal and Polyclonal Antibodies Research
- Chemical Synthesis and Analysis
- Immunotherapy and Immune Responses
- MicroRNA in disease regulation
- Click Chemistry and Applications
- Supramolecular Self-Assembly in Materials
- HIV Research and Treatment
- Biopolymer Synthesis and Applications
- Glycosylation and Glycoproteins Research
- Cancer Research and Treatments
- Dendrimers and Hyperbranched Polymers
- Radiopharmaceutical Chemistry and Applications
- Advanced Biosensing Techniques and Applications
- Cellular transport and secretion
- RNA and protein synthesis mechanisms
- Boron Compounds in Chemistry
- Peptidase Inhibition and Analysis
- Biotin and Related Studies
- Silk-based biomaterials and applications
Osaka Prefecture University
2015-2024
Osaka Metropolitan University
2022-2024
Tokyo Metropolitan University
2022-2023
Vanderbilt University
2020
Duke University
2020
Osaka Health Science University
2019
Kyoto University Institute for Chemical Research
2009-2018
Kyoto University
2009-2018
Kyoto Bunkyo University
2007-2018
Hyogo Medical University
2015
The use of membrane-permeable peptides as carrier vectors for the intracellular delivery various proteins and macromolecules modifying cellular function is well documented. Arginine-rich peptides, including those derived from human immunodeficiency virus 1 Tat protein, are among representative classes these vectors. internalization mechanism vector their protein conjugates was previously regarded separate endocytosis, but more recent reevaluations have concluded that endocytosis involved in...
Arginine-rich peptides, including octaarginine (R8), HIV-1 Tat, and branched-chain arginine-rich belong to one of the major classes cell-permeable peptides which deliver various proteins macromolecules cells. The importance endocytic pathways has recently been demonstrated in cellular uptake these peptides. We have previously shown that macropinocytosis is for organization F-actin accompanies this process. In study, using proteoglycan-deficient CHO cells, we membrane-associated proteoglycans...
The exact mechanisms by which arginine-rich cell-penetrating peptides enter cells are still the subject of debate. Here, we have analyzed in detail effects serum and extracellular concentration on internalization oligoarginines (R n; n = 4, 8, 12, 16). presence incubation medium had a major influence uptake R12 R16 but did not affect R4 R8 significantly. Incubation at 37 degrees C with serum-containing showed that majority labeling was confined to punctate endocytic structures. Performing...
Over the last 20 years, researchers have designed or discovered peptides that can permeate membranes and deliver exogenous molecules inside a cell. These peptides, known as cell-penetrating (CPPs), typically consist of 6–30 residues, including HIV TAT peptide, penetratin, oligoarginine, transportan, TP10. Through chemical conjugation noncovalent complex formation, these structures successfully bioactive membrane-impermeable into cells. CPPs also gained attention an attractive vehicle for...
Exosomes, which are approximately 100 nm vesicles secreted by cells, have been studied with respect to cell-to-cell communication, disease diagnosis, and intracellular delivery. The cellular uptake of exosomes occurs endocytosis; however, the cytosolic release efficiency encapsulated molecules inside cells is low. To address this issue, here we demonstrate a simple technique for enhancing combining pH-sensitive fusogenic peptide fusion endosomal exosomal membranes cells. This method...
Abstract Exosomes are approximately 100-nm vesicles that consist of a lipid bilayer cellular membranes secreted in large quantities from various types normal and disease-related cells. Endocytosis has been reported as major pathway for the uptake exosomes; however, detailed mechanisms their still unknown. Here, we demonstrate active induction macropinocytosis (accompanied by actin reorganisation, ruffling plasma membrane engulfment volumes extracellular fluid) stimulation cancer-related...
One of the recent hot topics in peptide-related chemical biology research is potential cell-penetrating peptides (CPPs). Owing to their ability deliver exogenous molecules into cells easily and effectively, flexible design that allows transporters comprise various structures functions, cell studies clinical applications, CPPs have been attracting enormous interest among researchers related fields. Consequently, publications on increased significantly. Although there are many types with...
Liposomes are one of the most promising systems for selective cellular targeting via introduction specific ligands cell-surface receptors. After being taken up by cells, these liposomes usually follow intracellular pathways receptor-mediated endocytosis. Control intracelluar trafficking is required optimized drug delivery. In this study, we elucidated fate transferrin-modified and succeeded in altering it introducing pH-sensitive fusogenic peptide, GALA (WEAALAEALAEALAEHLAEALAEALEALAA)....
Delineating the mechanisms by which cell-penetrating peptides, such as HIV-Tat peptide, oligoarginines and penetratin, gain access to cells has recently received intense scrutiny. Heightened interest in these entities stems from their ability enhance cellular delivery of associated macromolecules, genes proteins, suggesting that they may have widespread applications drug-delivery vectors. Proposed uptake include energy-independent plasma membrane translocation energy-dependent vesicular...
Endocytosis has been implicated in the cellular uptake of arginine-rich, cell-penetrating peptides (CPPs). However, accumulating evidence suggests that certain conditions allow direct, non-endocytic penetration arginine-rich through plasma membrane. We previously showed Alexa Fluor 488-labeled dodeca-arginine (R12-Alexa488) directly enters cells at specific sites on membrane and subsequently diffuses throughout cells. In this study, we found peptide influx was accompanied by formation...
Extracellular vesicles (EVs) including exosomes have been shown to play crucial roles in cell-to-cell communication because of their ability carry biofunctional molecules (e.g., microRNAs and enzymes). EVs also pharmaceutical advantages are highly anticipated be a next-generation intracellular delivery tool. Here, we demonstrate an experimental technique that uses arginine-rich cell-penetrating peptide (CPP)-modified induce active macropinocytosis for effective cellular EV uptake....
Arginine-rich cell-penetrating peptides (CPPs) such as Tat and oligoarginine have been widely used carriers for intracellular delivery of bioactive molecules. Despite accumulating evidence involvement endocytosis in the cellular uptake arginine-rich CPPs, primary cell-surface receptors these peptide that would initiate endocytic processes leading to cargoes remained poorly understood. Our previous attempt identify membrane octa-arginine (R8) peptide, one representative using...
Abstract Background As the application of silica nanomaterials continues to expand, increasing chances its exposure human body and potential harm are anticipated. Although toxicity is assumed be affected by their physio-chemical properties, including size surface functionalization, molecular mechanisms remain unclear. We hypothesized that analysis intracellular localization particles subsequent signaling could reveal a novel determinant inflammatory response against with different...
A basic peptide derived from HIV-1 Tat has been reported to have the ability translocate through cell membranes and bring exogenous proteins into cells. We demonstrated that these features could be observed among many arginine-rich peptides, presence of a ubiquitous internalization mechanism for oligopeptides suggested. In this report, we report are also applicable peptides having branched-chain structures. Peptides arginine residues on four branched chains (Rn)4 [n (number residues) = 0−6]...
Arginine-rich cell-penetrating peptides (CPPs), including human immunodeficiency virus type 1 (HIV-1) Tat (48-60) and oligoarginines, have been applied as carriers for delivery of cargo molecules, because their capacity to internalize into cells penetrate biological membranes. Despite the fact that they extensively studied, factors required efficient internalization CPPs are still unclear. In this report, we evaluated efficiencies seven derived from DNA/RNA-binding peptides, discovered a...