A5045692779- DNA Repair Mechanisms
- DNA and Nucleic Acid Chemistry
- CRISPR and Genetic Engineering
- RNA Interference and Gene Delivery
- Carcinogens and Genotoxicity Assessment
- Advanced biosensing and bioanalysis techniques
- Virus-based gene therapy research
- RNA and protein synthesis mechanisms
- Cardiac and Coronary Surgery Techniques
- RNA modifications and cancer
- Bacteriophages and microbial interactions
- Epigenetics and DNA Methylation
- Biochemical and Molecular Research
- Cardiac Valve Diseases and Treatments
- Bacterial Genetics and Biotechnology
- Aortic Disease and Treatment Approaches
- RNA regulation and disease
- Molecular Biology Techniques and Applications
- Electrospun Nanofibers in Biomedical Applications
- Mechanical Circulatory Support Devices
- Tissue Engineering and Regenerative Medicine
- Sperm and Testicular Function
- Genomics and Chromatin Dynamics
- Animal Genetics and Reproduction
- Cancer-related Molecular Pathways
Hiroshima University
2016-2025
Daido University
2020-2024
Saitama Medical University
2024
Social Insurance Saitama Chuo Hospital
2024
Asahikawa Medical University
2020-2024
Western Digital (Japan)
2024
Hiroshima Minami High School
2020
Ehime University
2011-2018
Matsuyama University
2012-2018
Nagoya City University
2003-2017
Liposomes are one of the most promising systems for selective cellular targeting via introduction specific ligands cell-surface receptors. After being taken up by cells, these liposomes usually follow intracellular pathways receptor-mediated endocytosis. Control intracelluar trafficking is required optimized drug delivery. In this study, we elucidated fate transferrin-modified and succeeded in altering it introducing pH-sensitive fusogenic peptide, GALA (WEAALAEALAEALAEHLAEALAEALEALAA)....
The possibility that Escherichia coliMutT and human MTH1 (hMTH1) hydrolyze oxidized DNA precursors other than 8-hydroxy-dGTP (8-OH-dGTP) was investigated. We report here hMTH1 hydrolyzed 2-hydroxy-dATP (2-OH-dATP) 8-hydroxy-dATP (8-OH-dATP), forms of dATP, but not (R)-8,5′-cyclo-dATP, 5-hydroxy-dCTP, 5-formyl-dUTP. kinetic parameters indicated 2-OH-dATP more efficiently with higher affinity 8-OH-dGTP. 8-OH-dATP as preferential hydrolysis over 8-OH-dGTP observed at all the pH values tested...
We found that hydroxylation occurs at the C-2 position of adenine by oxygen radical treatment (Fe2+-EDTA) dA, dATP, and single- double-stranded DNA. This oxidatively damaged base, 2-hydroxyadenine, was produced 3-6-fold 40-fold less than 8-hydroxyguanine when monomers polynucleotides, respectively, were treated. To determine whether nucleotide, 2-hydroxydeoxyadenosine triphosphate (2-OH-dATP), is incorporated into a growing DNA, to reveal kinds nucleotides opposite which 2-OH-dATP...
An oligodeoxyribonucleotide containing 8-hydroxyadenine (OH8Ade) was chemically synthesized and single- double-stranded c-Ha-ras gene fragments with OH8Ade at the second position of codon 61 were prepared. The single-stranded ras fragment used as a template for in vitro DNA synthesis Klenow Escherichla coli polymerase I, Taq polymerase, rat β mouse α. former two enzymes exclusively incorporated dTMP opposite 0H8Ade. polymerases p misinserted dGMP, dAMP respectively. constructed using...
Journal Article 8-Hydroxyguanine (7,8-dihydro-8-oxoguanine) in hot spots of the c-Ha-ras gene: effects sequence contexts on mutation spectra Get access Hiroyuki Kamiya, Kamiya 1 1Present address: Department Environmental Oncology, Institute Industrial Ecological Science, University Occupational and Health, Iseigaoka 1-1, Yahatanishi-ku, kitakyushu 807, Japan Search for other works by this author on: Oxford Academic PubMed Google Scholar Naoko Murata-Kamiya, Murata-Kamiya Shiro Koizume,...
Journal Article Design of RNA enzymes distinguishing a single base mutation in Get access Makoto Koizumi, Koizumi Faculty Pharmaceutical Sciences, Hokkaido UniversitySapporo 060, Japan Search for other works by this author on: Oxford Academic PubMed Google Scholar Yoji Hayase, Hayase Shigenori Iwai, Iwai Hiroyuki Kamiya, Kamiya Hideo Inoue, Inoue Eiko Ohtsuka Nucleic Acids Research, Volume 17, Issue 11 September 1989, Pages 7059–7069, https://doi.org/10.1093/nar/17.17.7059 Published: 1989...
We have developed a new strategy for the evaluation of mutagenicity damaged DNA precursor (deoxyribonucleoside 5′-triphosphate) in <i>Escherichia coli</i>. 8-Hydroxydeoxyguanosine triphosphate (8-OH-dGTP) and 2-hydroxydeoxyadenosine (2-OH-dATP) were chosen this study because they appear to be formed abundantly by reactive oxygen species cells. introduced oxidatively nucleotides into competent <i>E. coli</i> selected mutants chromosomal <i>lacI</i> gene. Both induced<i>lacI</i> gene mutations...
ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTSynthesis of a Phosphoramidite Coupling Unit the Pyrimidine (6−4) Pyrimidone Photoproduct and Its Incorporation into OligodeoxynucleotidesShigenori Iwai, Masato Shimizu, Hiroyuki Kamiya, Eiko OhtsukaView Author Information Biomolecular Engineering Research Institute 6-2-3 Furuedai, Suita, Osaka 565, Japan Faculty Pharmaceutical Sciences Hokkaido University, Kita-ku, Sapporo 060, JapanCite this: J. Am. Chem. Soc. 1996, 118, 32,...
Abstract The synthesis of the triphosphates 4′-thiouridine and 4′-thiocytidine, 4′-thioUTP (7; thioUTP) 4′-thioCTP (8; thioCTP), their utility for SELEX (systematic evolution ligands by exponential enrichment) is described. new nucleoside triphosphate (NTP) analogs 7 8 were prepared from appropriately protected -cytidine derivatives using one-pot method reported J. Ludwig F. Eckstein [(1989) Org. Chem ., 54, 631–635]. Because requires both in vitro transcription reverse transcription, we...
Abstract Cancer cells produce vast quantities of reactive oxygen species, leading to the accumulation toxic nucleotides as 8-oxo-7,8-dihydro-2′-deoxyguanosine 5′-triphosphate (8-oxo-dGTP). The human MTH1 protein catalyzes hydrolysis 8-oxo-dGTP, and cancer are dependent on for their survival. inhibitors possible candidates a class anticancer drugs; however, reliable screening system using live has not been developed. Here we report visualization method 8-oxo-dGTP its related in living cells....