A5087477950- Extracellular vesicles in disease
- Air Quality and Health Impacts
- MicroRNA in disease regulation
- Heavy Metal Exposure and Toxicity
- Energy and Environment Impacts
- Trace Elements in Health
- Adipose Tissue and Metabolism
- Nutrition and Health in Aging
- Asthma and respiratory diseases
- Muscle Physiology and Disorders
- Allergic Rhinitis and Sensitization
- Health and Medical Research Impacts
- Pesticide and Herbicide Environmental Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Epigenetics and DNA Methylation
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Noise Effects and Management
- Insects and Parasite Interactions
- Neurological Disease Mechanisms and Treatments
- EU Law and Policy Analysis
- Inhalation and Respiratory Drug Delivery
- Anesthesia and Neurotoxicity Research
- Environmental Toxicology and Ecotoxicology
- Circular RNAs in diseases
- Mercury impact and mitigation studies
Columbia University
2017-2024
New York Proton Center
2022
Center for Health and Gender Equity
2021
National Institute of Environmental Health Sciences
2021
Vanderbilt University
2020
Duke University
2020
Boston University
2017
Drexel University
2017
<h3>Importance</h3> School and classroom allergens particles are associated with asthma morbidity, but the benefit of environmental remediation is not known. <h3>Objective</h3> To determine whether use a school-wide integrated pest management (IPM) program or high-efficiency particulate air (HEPA) filter purifiers in classrooms improve symptoms students active asthma. <h3>Design, Setting, Participants</h3> Factorial randomized clinical trial IPM HEPA was conducted from 2015 to 2020 (School...
DNA methyltransferase 1 (DNMT1) is a multidomain protein believed to be involved only in the passive transmission of genomic methylation patterns via maintenance methylation. The mechanisms that regulate DNMT1 activity and targeting are complex poorly understood. We used embryonic stem (ES) cells investigate function uncharacterized bromo-adjacent homology (BAH) domains glycine–lysine (GK) repeats join regulatory catalytic DNMT1. removed BAH by means CRISPR/Cas9-mediated deletion within...
The physiological and pathophysiological roles of extracellular vesicles (EVs) have become increasingly recognized, making the EV field a quickly evolving area research. There are many different methods for isolation, each with distinct advantages disadvantages that affect downstream yield purity EVs. Thus, characterizing prep isolated from given source by chosen method is important interpretation results comparison across laboratories. Various exist determining size quantity EVs, which can...
The physiological and pathophysiological roles of extracellular vesicles (EVs) have become increasingly recognized, making the EV field a quickly evolving area research. There are many different methods for isolation, each with distinct advantages disadvantages that affect downstream yield purity EVs. Thus, characterizing prep isolated from given source by chosen method is important interpretation results comparison across laboratories. Various exist determining size quantity EVs, which can...
To confirm the presence of extracellular vesicles (EVs) in cell-free saliva (CFS) children with asthma and describe isolated EV population.A pooled sample CFS EVs from 180 participants using ExoQuick-TC was examined downstream analyses. Transmission electron microscopy (TEM) used to EVs. Nanoparticle tracking analysis (NTA) single particle interferometric reflectance imaging sensing (SP-IRIS) fluorescence were for sizing, counting, phenotyping Capillary immunoassays protein quantitation.TEM...
Sarcopenia, the age-associated decline in skeletal muscle mass and strength, has long been considered a disease of only, but accumulating evidence suggests that sarcopenia could originate from neural components controlling muscles. To identify early molecular changes nerves may drive initiation, we performed longitudinal transcriptomic analysis sciatic nerve, which governs lower limb muscles, aging mice.
Abstract Human endogenous retroviruses (HERVs) are transposable genomic elements generally repressed through DNA methylation. HERVs can be demethylated and expressed in response to environmental stimuli. Therefore, more research is needed understand the influence of exposures on HERV Air pollutants commonly linked with global hypomethylation, as comprise nearly 8% repetitive human genome, our objective was examine association between air pollutant exposure We investigated 180 students asthma...
While the health effects of air pollution and temperature are widely studied, molecular poorly understood. Extracellular microRNAs (ex-miRNAs) have potential to serve as diagnostic or prognostic biomarkers and/or act intercellular signaling molecules that mediate environmental exposures on outcomes. We examined relationship between short-term exposure ambient ex-miRNA profiles participants in Normative Aging Study (NAS) from 1999 2015. Our were defined same-day, two-day, three-day, one-week,...
Gulf War illness (GWI), which afflicts at least 25% of veterans who served in the 1990-1991 war Persian Gulf, is thought to be caused by deployment exposures various neurotoxicants, including pesticides, anti-nerve gas pills, and low-level nerve agents sarin/cyclosarin. GWI a multisymptom disorder characterized fatigue, joint pain, cognitive problems, gastrointestinal complaints. The most prominent symptoms (memory poor attention/concentration, chronic headaches, mood alterations, impaired...
Aging | doi:10.18632/aging.204268. Nicole Comfort, Haotian Wu, Peter De Hoff, Aishwarya Vuppala, Pantel S. Vokonas, Avron Spiro, Marc Weisskopf, Brent A. Coull, Louise C. Laurent, Andrea Baccarelli, Joel Schwartz
TPS 651: Air pollution exposure modeling 1, Exhibition Hall, Ground floor, August 27, 2019, 3:00 PM - 4:30 Background: Saliva collection is fast, easy, inexpensive, and non-invasive saliva easy to transport, making it an ideal biofluid for epidemiological research. However, there a relatively low concentration of biomarkers in when compared other biofluids. Thus, successful measurement salivary analytes such as extracellular vesicle (EV) microRNA requires optimal collection, processing,...
The United States (US) must strategically invest in diversifying its biomedical workforce to retain global leadership research and reduce racial ethnic disparities the US. under-representation of minority groups sciences is influenced by National Institutes Health (NIH) grant funding process which relies heavily on peer review subject bias. Despite recent initiatives combat structural racism within NIH, NIH has done little rectify allocation that have been known for over a decade. In this...
Abstract Background Sarcopenia, the age-associated decline in skeletal muscle mass and strength, has long been considered a disease of only, but accumulating evidence suggests that sarcopenia could originate from neural components controlling muscles. To identify early molecular changes efferent nerves may drive initiation, we performed longitudinal transcriptomic analysis sciatic nerve aging mice. Methods Sciatic gastrocnemius were obtained young adult, middleaged, old, sarcopenic (5,18, 21...
Extracellular Vesicles and Circulating Nucleic Acids is an open access journal, focusing on extracellular vesicles circulating nucleic acids including DNA, RNA, miRNA their therapeutic use.
OBJECTIVES/GOALS: This study aims to develop a method examine whether blood-borne CNS-EV metal cargoes can serve as reliable biomarkers of CNS load and reveal link between ALS development (i.e., neurodegenerative disease development). METHODS/STUDY POPULATION: CNS-EVs were isolated from human blood plasma samples via direct immunoprecipitation using biotinylated antibodies for proteins known be expressed in neurons (Contactin-2, i.e.) astrocytes (glial-glutamate-aspartate-transporter, i.e.,...
Background and aims: Many environmental exposures (e.g., metals, pesticides) accumulate in central nervous system (CNS) tissues are associated with adverse neurological outcomes. There is a great need for peripheral biomarkers that could provide insight into the CNS burden of these to confirm etiologic role neurotoxic exposures. Our research group has observed cells extruding toxicants via extracellular vesicles (EVs) characterizing viability CNS-derived EVs isolated from blood draws as...
Background and Aim Metals have previously been associated with adverse effects in multiple organ systems. However, the molecular mechanisms by which they cause these are not fully elucidated. MicroRNAs short non-coding RNA sequences that play an important role regulation of gene expression may be a mechanism metals influence health. Methods The study population consisted of496 persons (with 694 samples) enrolled Normative Aging Study (NAS). This cohort began recruiting male veteran who lived...
Background and Aims: Human endogenous retroviruses (HERVs) are transposable genomic elements. Usually repressed through DNA methylation, HERVs can be demethylated in response to environmental stimuli, their expression may lead dysregulation of the immune system. More research is needed understand influence exposures on HERV as air pollutants commonly linked with system dysregulation, our objective was examine association pollutant exposure demethylation. Methods: The sample included 180...
Background and Aims: Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative condition, though environmental exposure to metals has been associated with ALS, there are no existing biomarkers for these potentially causal exposures – biomarker toxicant load in the central nervous system greatly needed. Upon toxic vitro, (CNS) astrocytes, critical metallohomeostasis, were observed produce extracellular vesicles loaded metals, as cellular elimination pathway. Metals known bioaccumulate...
BACKGROUND AND AIM: Previous research have suggested the link between air pollution and epigenetic changes such as microRNA expression. In this study, we examined whether short- long-term exposures to PM2.5, NO2, O3 are associated with in profile of patients Normative Aging Study (NAS). METHODS: The study population consisted persons enrolled NAS which has followed male veterans every three five years since 1963. Our period interest spanned from 1999 2015. Blood samples collected...
BACKGROUND AND AIM: Metal exposure is linked to amyotrophic lateral sclerosis (ALS) but biomarkers of central nervous system (CNS) metal burden are lacking. Here, we tested the use blood-isolated CNS-derived extracellular vesicles (EVs) as and disease progression in ALS. Specifically, examined whether: 1) cultured brain cells can eliminate metals via EV release, 2) levels ALS patient CNS-EVs predict load their spinal cord, 3) differences CNS-EV associated with (ongoing). METHODS: were...